NCT04898088

Brief Summary

Our aim in this study is to determine the positive effect of stem cell therapy applied on critically ill patients with coronavirus infection on DNA repair genes. Patients diagnosed with COVID-19 infection are divided into two equal (n:30) groups. Group-1(n/15): Patients in critically ill condition receiving conventional therapy, Group-2 (n/15): Patients in critically ill condition receiving conventional therapy and systemically transplanted MSCs. The DNA repair pathway will be examined as 11 genes in 5 different parts. Investigated parameters:

  1. 1.Base excision repair
  2. 2.Nucleotide excision repair
  3. 3.Recombinational repair
  4. 4.Mismatch repair
  5. 5.Direct reversal Investigated parameters: broad biochemical analysis, apoptosis, clinical outcome, and mortality rates.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
30

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jan 2020

Shorter than P25 for not_applicable

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2020

Completed
8 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 30, 2020

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

September 30, 2020

Completed
8 months until next milestone

First Submitted

Initial submission to the registry

May 21, 2021

Completed
3 days until next milestone

First Posted

Study publicly available on registry

May 24, 2021

Completed
Last Updated

May 24, 2021

Status Verified

May 1, 2021

Enrollment Period

8 months

First QC Date

May 21, 2021

Last Update Submit

May 21, 2021

Conditions

Keywords

COVID-19Critical CaseDNA RepairDAN damage

Outcome Measures

Primary Outcomes (4)

  • Expression of PARP1 gene as indicator of base excision repair

    Expression of PARP1 gene as indicator of base excision repair

    6 months

  • Expression of genes ATM, RAD51, RAD52 and WRN as indicator of Recombinational repair

    Expression of genes ATM, RAD51, RAD52 and WRN as indicator of Recombinational repair

    6 months

  • Expression of genes RAD23B and ERCC1 as indicator of Nucleotide excision repair

    Expression of genes RAD23B and ERCC1as indicator of Nucleotide excision repair

    6 months

  • Expression of genes MLH1, MSH2 and MSH6as indicator of Mismatch repair

    Expression of genes MLH1, MSH2 and MSH6 as indicator of Mismatch repair

    6 months

Study Arms (2)

Conventional Therapy

EXPERIMENTAL
Biological: Mesenchymal Stem Cells Transplantation

Conventional Therapy with Add-On MSC therapy

EXPERIMENTAL
Biological: Mesenchymal Stem Cells Transplantation

Interventions

Mesenchymal Stem Cells Transplantation applied as three intravenous infusions with 30 days intervals

Conventional TherapyConventional Therapy with Add-On MSC therapy

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • years old male/female.
  • Obtaining informed consent from him or his legal relative.
  • Confirmed COVID-19 related severe ARDS cases.

You may not qualify if:

  • pregnant, malignant tumours, the ones who has confirmed co-infection; history of using long-term immunosuppressive agents

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Istinye University

Istanbul, 34010, Turkey (Türkiye)

Location

SBÜ Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi

Istanbul, 34147, Turkey (Türkiye)

Location

MeSH Terms

Conditions

COVID-19

Condition Hierarchy (Ancestors)

Pneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 21, 2021

First Posted

May 24, 2021

Study Start

January 1, 2020

Primary Completion

August 30, 2020

Study Completion

September 30, 2020

Last Updated

May 24, 2021

Record last verified: 2021-05

Locations