Influence of Sex and Training on de Novo Muscle Protein Synthesis

NCT04887883 | Completed

• 2 other identifiers: TUT studyRGPIN-2015-04521
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Acute exercise increases the incorporation of dietary amino acids into de novo myofibrillar proteins after a single meal in controlled laboratory studies in males. It is unclear if this extends to free-living settings or is influenced by training or sex. Over 24 h in a free-living setting, the investigators determined the effect of training status and sex on dietary phenylalanine incorporation into contractile myofibrillar and noncontractile sarcoplasmic proteins after exercise.

Conditions

Amino Acids; Dietary Protein; Sex; Resistance Exercise

Keywords

intrinsically labeled proteins; muscle protein synthesis

Outcome Measures

Primary Outcomes (1)

• Dietary fate of amino acids into myofibrillar and sarcoplasmic proteins

  MPE

  The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training

Secondary Outcomes (2)

• Amino acid transporter protein content

  0 and 24 hours after acute resistance exercise before and after training

• mTOR localization with capillaries

  0 and 24 hours after acute resistance exercise before and after training

Study Arms (2)

Males

10 young healthy biological males aged 18 - 30 y

Behavioral: Resistance exercise

Females

10 young healthy biological females aged 18 - 30 y

Behavioral: Resistance exercise

Interventions

Resistance exercise BEHAVIORAL

Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.

Females; Males

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

: Ten recreationally active and healthy young males and females

You may qualify if:

• Participants were included in the study after reporting not engaging in whole body RE or plyometrics in the past 3 months and had a BMI between 18.5-27.5 kg/m2.
• Females were included if they had a regular menstrual cycle with the last 3 months and

You may not qualify if:

• Participants were excluded if they: i) consumed tobacco and/or illicit anabolic drug use (e.g. testosterone, growth hormones); ii) were a vegan or had a nut allergy and; iii) participated in a study within the past year involving stable isotopes.
• Females were excluded if they used oral contraceptives and/or discontinued their use within the last 3 months.

Sponsors & Collaborators

• University of Toronto: lead
• Natural Sciences and Engineering Research Council, Canada: collaborator

Study Sites (1)

Goldring Centre for High Performance Sport

Toronto, Ontario, M9L 1C5, Canada

Location

Related Publications (1)

• Abou Sawan S, Hodson N, Malowany JM, West DWD, Tinline-Goodfellow C, Brook MS, Smith K, Atherton PJ, Kumbhare D, Moore DR. Trained Integrated Postexercise Myofibrillar Protein Synthesis Rates Correlate with Hypertrophy in Young Males and Females. Med Sci Sports Exerc. 2022 Jun 1;54(6):953-964. doi: 10.1249/MSS.0000000000002878. Epub 2022 Jan 25.

  PMID: 35081094 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

Muscle biopsies, blood and saliva

MeSH Terms

Conditions

Coitus

Interventions

Resistance Training

Condition Hierarchy (Ancestors)

Sexual Behavior; Behavior

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Daniel Moore, PhD

  University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: May 5, 2021
• First Posted: May 14, 2021
• Study Start: January 1, 2019
• Primary Completion: September 1, 2019
• Study Completion: September 1, 2019
• Last Updated: May 14, 2021

Record last verified: 2021-05

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)