Genetic Diversity of Toxoplasma Gondii in Cancer Patients
1 other identifier
observational
100
1 country
1
Brief Summary
I. Evaluation of T. gondii infection in cancer patients using different serological markers. II. Studying genetic lineages infecting cancer patients in Sohag Governorate to predict clinical course and therapeutic needs using B1 and RE genes.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P50-P75 for all trials
Started Jun 2020
Typical duration for all trials
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
June 1, 2020
CompletedPrimary Completion
Last participant's last visit for primary outcome
January 30, 2021
CompletedFirst Submitted
Initial submission to the registry
April 4, 2021
CompletedFirst Posted
Study publicly available on registry
April 6, 2021
CompletedStudy Completion
Last participant's last visit for all outcomes
December 1, 2022
CompletedJanuary 13, 2022
January 1, 2022
8 months
April 4, 2021
January 12, 2022
Conditions
Outcome Measures
Primary Outcomes (2)
Isolation and genotyping of T. gondii from blood of Cancer patients in Sohag Governorate, Egypt using PCR and DNA sequencing of Toxoplasma gondii B1 & RE genes.
Using PCR and DNA sequencing
up to 1 year
Evaluation of T. gondii infection in cancer patients using ELISA.
IgG, IgM.
up to 1 year
Study Arms (2)
Cancer patients
50 cancer patients referred to the Oncology Department, Faculty of medicine, Sohag University.
Healthy controls
50 healthy controls will be recruited in the study.
Interventions
ELISA is is a test that detects and measures toxoplasma IgG and IgM antibodies in blood.
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.
DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information a cell needs to assemble protein and RNA molecules. DNA sequence information is important to scientists investigating the functions of genes.
Eligibility Criteria
50 cancer patients referred to the Oncology Department, Faculty of medicine, Sohag University and 20 healthy controls will be recruited in the study. Relevant demographic and clinical data will be obtained from all participants. Written consents will be obtained from each participant after clear full explanation about the procedures and their significance.
You may qualify if:
- Hematologic cancers on treatment
- Solid Cancers on treatment
You may not qualify if:
- Patients with other immune compromise states eg. DM, autoimmune diseases
- Patients who are HIV positive
Contact the study team to confirm eligibility.
Sponsors & Collaborators
- Sohag Universitylead
Study Sites (1)
Sohag University Hospitals
Sohag, Egypt
Biospecimen
Blood samples in EDTA tubes for molecular detection of Toxoplasma gondii gene then for sequencing.
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Eman FF Mohammed, Ass lecturer
Sohag University
Central Study Contacts
Study Design
- Study Type
- observational
- Observational Model
- CASE CONTROL
- Time Perspective
- RETROSPECTIVE
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Teaching assistant of medical parasitology
Study Record Dates
First Submitted
April 4, 2021
First Posted
April 6, 2021
Study Start
June 1, 2020
Primary Completion
January 30, 2021
Study Completion
December 1, 2022
Last Updated
January 13, 2022
Record last verified: 2022-01