Lemon Verbena Extract Oxidative Stress and Muscle Damage
Evaluation of the Efficacy of Supplementation With Lemon Verbena Extract in Improving
1 other identifier
interventional
60
1 country
1
Brief Summary
In the present study, investigators investigated whether supplementation with lemon verbena extract (LVE) could improve muscle damage and biochemical indicators after exhaustive exercise challenge. Based on maximum jump heights, 60 subjects (30 males and 30 females) were equally divided into a placebo group (0 mg/human/day) and an LVE supplement group (400 mg/human/day), with gender-equal distribution. All subjects started supplementation 10 days before exhaustive exercise and continued it until all tests were completed. Before the intervention, after the exhaustive exercise, and on the following 3 days, the participants underwent 12-minute Cooper running/walking; blood collection; assessments of pain, muscle stiffness, maximum jump heights, and isometric maximum muscle strength; and anaerobic strength tests.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P25-P50 for not_applicable
Started May 2020
Shorter than P25 for not_applicable
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
May 28, 2020
CompletedPrimary Completion
Last participant's last visit for primary outcome
July 28, 2020
CompletedStudy Completion
Last participant's last visit for all outcomes
August 13, 2020
CompletedFirst Submitted
Initial submission to the registry
January 25, 2021
CompletedFirst Posted
Study publicly available on registry
February 8, 2021
CompletedMarch 10, 2021
March 1, 2021
2 months
January 25, 2021
March 8, 2021
Conditions
Keywords
Outcome Measures
Primary Outcomes (5)
inflammation of IL-6 level
To assess inflammation indicators, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Assess serum IL-6 levels (pg/ml) to monitor physiological fitness. Used commercial kit human IL-6 immunoassay (R\&D system, Minnesota, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis
10 days
Clinical Biochemistry of CK level
To assess muscle damage, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Serum CK (U/L), were assessed for monitoring physiological adaptation. All biochemical indices were assessed using an autoanalyzer (Hitachi 7060, Tokyo, Japan).
10 days
Stress hormones 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) in Urine
Subjects' urine was collected 24 hours after the exhaustive exercise for analysis. Used commercial kit human 8-OHdG (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis
10 days
Assessment of muscle stiffness
3 h, 24h, 48h after exhausting exercise, use Myoton machine to evaluate muscle stiffness (N/m) (Myoton, Tallinn, Estonia)
10 days
Visual Analogue Scale (VAS) evaluation
3 h, 24h, 48h after exhausting exercise, use VAS questionnaire to record muscle pain. the minimum is 0 and maximum is 10, and whether higher scores mean more pain
10 days
Study Arms (2)
Placebo
PLACEBO COMPARATOR0 mg LVE/capsule, 2capsule/day, 14days
Lemon Verbena extract
EXPERIMENTAL200 mg LVE/capsule, 2capsule/day, 14days
Interventions
Each capsule contained 200 mg of lemon verbena extract 2 capsules/day, 14 days
Each capsule contained 0 mg of lemon verbena extract 2 capsules/day, 14 days
Eligibility Criteria
You may qualify if:
- health
- Must be able to swallow tablets
You may not qualify if:
- non-smokers
- no musculoskeletal
- medical
- metabolic disease
- women not on the reproductive cycle
- high blood pressure, asthma
- skeletal neuromuscular injuries in the upper or lower extremities
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
National Taiwan Sport University
Taoyuan District, 333, Taiwan
Related Publications (1)
Lee MC, Hsu YJ, Ho CS, Chang CH, Liu CW, Huang CC, Chiang WD. Evaluation of the Efficacy of Supplementation with Planox(R) Lemon Verbena Extract in Improving Oxidative Stress and Muscle Damage: A Randomized Double-Blind Controlled Trial. Int J Med Sci. 2021 May 3;18(12):2641-2652. doi: 10.7150/ijms.60726. eCollection 2021.
PMID: 34104096DERIVED
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Chi-Chang Huang, Ph.D
PI
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- NON RANDOMIZED
- Masking
- TRIPLE
- Who Masked
- PARTICIPANT, CARE PROVIDER, INVESTIGATOR
- Purpose
- TREATMENT
- Intervention Model
- PARALLEL
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- PI
Study Record Dates
First Submitted
January 25, 2021
First Posted
February 8, 2021
Study Start
May 28, 2020
Primary Completion
July 28, 2020
Study Completion
August 13, 2020
Last Updated
March 10, 2021
Record last verified: 2021-03