Preference of Protein Intake and FTO rs1558902 Gene Polymorphism Among Women Obese

NCT04740528 | Completed DMC

• 1 other identifier: FTO
• Study Type: observational
• Target: 130
• Locations: 1 country
• Sites: 1

Brief Summary

The concept personalized gene-based nutrition combines genetic information with specific dietary intake that is crucial in managing obesity. Obesity experienced by adult women is generally caused by inapproriate diet and sedentary lifestyle. Variations in the fat mass and obesity-related gene (FTO) has been linked with susceptibility to obesity, but diet seems to change the relationship. A single nucleotide polymorphism (SNP) in the fat mass and obesity-associated gene (FTO) is a potent predictor of human obesity. Higher protein diets were more appropriate than standard protein diets, and frequently recommended as a weight loss plan as it prevents the loss of lean tissue mass. Nevertheless, high intakes of proteins may adversely affect metabolic functions. Multi studies have explored associated FTO polymorphisms with obesity in different populations. However, the contribution of the FTO common variants to obesity is controversial in Asian people, some studies showed rs1558902 was statistically associated with BMI, but other results reported FTO gene is not statistically associated with obesity. Given the diversity of Asian populations, the investigators generated a hypothesis whether relations between preference protein intake and FTO rs1558902 gene polymorphism exist in selected Indonesian obesity women

Conditions

Obesity Adult Onset

Keywords

woman Obese; fto-gen; rs1558902; protein intake

Outcome Measures

Primary Outcomes (2)

• fto gene rs 1558902

  Genomic DNA was extracted from 300 ml of buffy coat using spin colomn method. rs 1558902 were genotyped using TaqMan probes C\_\_30090620\_10

  day 1

• interleukin-6

  plasma IL-6 was determined by enzyme-linked immunosorbent method (EliKine™ Human IL-6 ELISA Kit)

  day 1

Interventions

fto gene testing DIAGNOSTIC_TEST

Blood samples were collected after a 12-h fasting by Prodia Laboratory for DNA isolation and biochemical analysis

Eligibility Criteria

• Age: 35 Years - 45 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

healthy woman obese and pre menopause

You may qualify if:

• Body Mass index \> 25 kg/m2
• waist circumference \> 80 cm

You may not qualify if:

• chronic disease
• have pregnancy
• consume diet supplement, drugs

Sponsors & Collaborators

• Universitas Diponegoro: lead

Study Sites (1)

Etika Ratna Noer

Semarang, Central Java, 50275, Indonesia

Location

Biospecimen

Retention: SAMPLES WITH DNA

whole blood specimen intravenous 5ml

Study Officials

• Etika Noer, PhD

  Diponegoro University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Department of Nutrition, Medical Faculty

Study Record Dates

• First Submitted: February 1, 2021
• First Posted: February 5, 2021
• Study Start: August 1, 2020
• Primary Completion: October 1, 2020
• Study Completion: November 30, 2020
• Last Updated: March 2, 2021

Record last verified: 2021-02

Locations

ID (1)