NCT04701229

Brief Summary

Myelodysplastic syndromes (MDS) are malignant hematopathies of the elderly characterized by persistent cytopenias and the presence of deregulated clonal hematopoiesis. The risk of progression to acute myeloid leukemia (AML) is variable. Acquired cytogenetic abnormalities are found in less than 50% of de novo cases and up to 80% in secondary MDS. The deletion of the long arm of chromosome 5 (written del(5q)) is the most common abnormality in MDS (15%). Del(5q) MDS has a good prognosis, with a median survival of 6 years and a 15% risk of progression to AML. However, their life expectancy is shorter than the general population, and the quality of life of patients is diminished. These treatments are not that effective over a long period of time or not well tolerated, and the majority of patients die from causes related to their MDS, such as infections (38%), progression to AML (15%), or bleeding (13%). Two genes, RBM22 and SLU7, coding for proteins of the same complex involved in splicing pre-messenger RNA are carried on the long arm of chromosome 5. We investigate the pronostic impact and the predictive value of the double haploinsufficiency of the RBM22 and SLU7 genes in del(5q) myelodysplastic syndromes isolated or not compared to the single haploinsufficiency of RBM22 and normal karyotype myelodysplastic syndromes.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
100

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Sep 2020

Typical duration for all trials

Geographic Reach
1 country

3 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

September 30, 2020

Completed
3 months until next milestone

First Submitted

Initial submission to the registry

January 6, 2021

Completed
2 days until next milestone

First Posted

Study publicly available on registry

January 8, 2021

Completed
1.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 30, 2022

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

September 30, 2023

Completed
Last Updated

January 8, 2021

Status Verified

January 1, 2021

Enrollment Period

2 years

First QC Date

January 6, 2021

Last Update Submit

January 6, 2021

Conditions

Keywords

RBM22SLU7transformation to acute myeloid leukemiaanemia

Outcome Measures

Primary Outcomes (1)

  • prognostic impact on anemia

    To evaluate the prognostic impact of the dual loss of an RBM22 and a SLU7 allele compared to the loss of an RBM22 allele alone on anemia, as measured by the hemoglobin level in the blood, between diagnosis and one year in patients with del5q myelodysplastic syndrome.

    retrospective study on data collected; 2 years

Secondary Outcomes (3)

  • prognostic impact on blood count

    retrospective study on data collected; 2 years

  • prognostic impact on progression to leukemia

    retrospective study on data collected; 2 years

  • impact on gene expression and splicing profile

    retrospective study on RNA collected; 2 years

Study Arms (5)

normal karyotype

control group

Genetic: somatic cytogenetic and genetic characterization

del5q-RBM22neg-SLU7neg

this group is characterized by its karyotype. It presents a 5q deletion, a loss of RBM22, a loss of SLU7.

Genetic: somatic cytogenetic and genetic characterization

del5q-RBM22neg-SLU7pos

this group is characterized by its karyotype. It presents a 5q deletion, a loss of RBM22 but no loss of SLU7

Genetic: somatic cytogenetic and genetic characterization

del5q-RBM22pos-SLU7neg

this group is characterized by its karyotype. It presents a 5q deletion, and no loss of RBM22, but a loss in SLU7

Genetic: somatic cytogenetic and genetic characterization

del5q-RBM22pos-SLU7pos

this group is characterized by its karyotype. It presents a 5q deletion, and no loss of RBM22 nor SLU7

Genetic: somatic cytogenetic and genetic characterization

Interventions

investigating the presence of some genes allelles (RBM22, SLU7, RBM27, other on chromosome 5) by FISH, and sequencing of a classical panel of myeloid genes including RBM22, SLU7, for somatic identification of genetic alterationss of the blasts.

del5q-RBM22neg-SLU7negdel5q-RBM22neg-SLU7posdel5q-RBM22pos-SLU7negdel5q-RBM22pos-SLU7posnormal karyotype

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients diagnosed with myelodysplastic syndromes and presenting a deletion of chromosome 5 in their bone marrow cells

You may qualify if:

  • Patients diagnosed with del5q MDS isolated or not
  • The clinical and biological data are known at the time of diagnosis.
  • The clinical and biological data are known 1 year after the diagnosis
  • Consent for the collection of samples for research purposes
  • Non-opposition obtained

You may not qualify if:

  • Patients under judicial protection (guardianship, ...)
  • Refusal to participate

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

CHRU de Brest

Brest, 29609, France

RECRUITING

Groupe Français de cytogénétique Hématologique

Paris, 75000, France

RECRUITING

Groupe Français des Myélodysplasies

Paris, 75000, France

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

cytogenetic pellets; bone marrow patients cells

MeSH Terms

Conditions

Myelodysplastic SyndromesChromosome 5q Deletion SyndromeAnemia

Condition Hierarchy (Ancestors)

Bone Marrow DiseasesHematologic DiseasesHemic and Lymphatic Diseases

Study Officials

  • Marie-Bérengère TROADEC

    CHRU Brest

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
RETROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 6, 2021

First Posted

January 8, 2021

Study Start

September 30, 2020

Primary Completion

September 30, 2022

Study Completion

September 30, 2023

Last Updated

January 8, 2021

Record last verified: 2021-01

Data Sharing

IPD Sharing
Will share

All collected data that underlie results in a publication

Shared Documents
STUDY PROTOCOL
Time Frame
Data will be available beginning three years and ending fifteen years following the publication
Access Criteria
Data access requests will be reviewed by the internal committee of Brest UH. Requestors will be required to sign and complete a data access agreement.

Locations