Acute Whey Protein And Casein Supplementation: Effect On Protein Metabolism After Resistance Exercise

NCT04648384 | Completed DMC

• 1 other identifier: Protein Supplementation
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The proteins of human breast milk are the most important endocrine signaling system that promotes neonatal growth by increasing the release of insulin, insulin-like growth factor-1 (IGF-1), and the leucine-mediated mammalian target of rapamycin complex 1- (mTORC1) signaling of pancreatic β-cells. Remarkably, the branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are involved in the growth-promoting effects of milk, protein biosynthesis, and metabolism because they physiologically stimulate insulin secretion The present study sought to investigate the effects of the combination of whey protein and casein in the ratio of 80:20 ("whey protein: casein" or "casein: whey protein") as breast milk proportion on the peak and the period of the permanence of branched-chain amino acids in the blood circulation, final metabolites of protein metabolism and delayed onset muscle soreness (DOMS) after a single bout of fasting or a resistance exercise session.

Conditions

Sports Nutrition; Protein Supplementation

Outcome Measures

Primary Outcomes (5)

• Change from baseline in plasma amino acids concentration

  Blood sample collected for analysis of BCAA concentration after blend protein or placebo supplementation expressed in (µmol/L)

  5 weeks

• Change from baseline in 1RM test

  Change of training load using RPE, time and load expressed in (a.u)

  5 weeks

• Change in protein metabolism

  Change in nitrogen excretion, and nitrogen balance after protein supplementation expressed in (g/24 hours)

  5 weeks

• Changes from baseline in macronutrient intake

  Change of macronutrient intake were calculated pre and post treatment expressed in (g/24 hours)

  5 weeks

• Change in delayed onset muscle soreness

  Change from baseline the delayed onset muscle soreness pre, post, 24 hours, 48 hours and 72 hours after treatment expressed in (a.u)

  5 weeks

Study Arms (3)

Protein Supplementation and Resistance exercise

EXPERIMENTAL

Protein supplementation composed of 20 gram of whey protein or casein in different proportions (80:20) and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition

Dietary Supplement: Blend Protein Supplementation; Other: Resistance Exercise

Placebo Supplementation

PLACEBO COMPARATOR

Placebo supplementation composed of 20 gram of Maltodextrin and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition

Dietary Supplement: Placebo Supplementation; Other: Resistance Exercise

Resistance Training

EXPERIMENTAL

Resistance Exercise will be performed before supplementation and is composed by 10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise.

Other: Resistance Exercise

Interventions

Blend Protein Supplementation DIETARY_SUPPLEMENT

Protein supplementation using 20 grams of whey protein or casein mix. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Also known as: Resistance Training

Protein Supplementation and Resistance exercise

Placebo Supplementation DIETARY_SUPPLEMENT

Placebo supplementation using 20 grams of Maltodextrin. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Also known as: Resistance Training

Placebo Supplementation

Resistance Exercise OTHER

10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Placebo Supplementation; Protein Supplementation and Resistance exercise; Resistance Training

Eligibility Criteria

• Age: 20 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy
• practice resistance training at least 3x a week

You may not qualify if:

• Smokers
• Muscle injures
• cardiovascular diseases
• Diabetes mellitus
• Lactose intolerance

Sponsors & Collaborators

• University of Sao Paulo: lead
• Fundação de Amparo à Pesquisa do Estado de São Paulo: collaborator
• Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.: collaborator
• Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (COLCIENCIAS): collaborator

Study Sites (1)

University of São Paulo, School of Physical Education and Sports of Ribeirão Preto.

Ribeirão Preto, São Paulo, 14049-900, Brazil

Location

Related Publications (1)

• Martinez Galan BS, Giolo De Carvalho F, Carvalho SCS, Cunha Brandao CF, Morhy Terrazas SI, Abud GF, Meirelles MSS, Sakagute S, Ueta Ortiz G, Marchini JS, Aristizabal JC, Cristini de Freitas E. Casein and Whey Protein in the Breast Milk Ratio: Could It Promote Protein Metabolism Enhancement in Physically Active Adults? Nutrients. 2021 Jun 23;13(7):2153. doi: 10.3390/nu13072153.

  PMID: 34201617 DERIVED

MeSH Terms

Interventions

Resistance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: November 9, 2020
• First Posted: December 1, 2020
• Study Start: March 20, 2016
• Primary Completion: July 20, 2019
• Study Completion: October 20, 2019
• Last Updated: December 2, 2020

Record last verified: 2020-11

Locations

BR (1)