NCT04567212

Brief Summary

The GINA 2020 guidelines suggest that asthma affects approximately 300 million persons worldwide. Even if there is a specific drug treatment for each stage of disease (From mild to severe) in some patients it is not efficacy and it culd be reated to the gender difference Polymorphisms seems to be involved in asthma (allergic or not) even if no data have been published concerning the role of gender in this clinical manifestation. The aim of this study is to assess whether genetic variations involved in the genes encoding the two key leukotriene enzymes, ALOX5 and LTC4S, and CysLTR1 are implicated in the sex difference of allergic asthma in a well-characterized patient cohort.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
50

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Aug 2020

Geographic Reach
1 country

2 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

August 1, 2020

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

September 11, 2020

Completed
17 days until next milestone

First Posted

Study publicly available on registry

September 28, 2020

Completed
1.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 20, 2021

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

December 30, 2021

Completed
Last Updated

November 23, 2020

Status Verified

November 1, 2020

Enrollment Period

1.3 years

First QC Date

September 11, 2020

Last Update Submit

November 20, 2020

Conditions

Keywords

asthmaallergyGenderpolymorphism

Outcome Measures

Primary Outcomes (1)

  • Expression of SNPs

    statistically significant difference (P\<0.05) in SNPs expression between male and female

    1 month

Secondary Outcomes (2)

  • Concentration of MicroRNA

    1 month

  • SNPs and age

    1 month

Study Arms (2)

Male-group

in this group we will enroll only male with asthma

Genetic: genetic evaluationOther: MicroRNA evaluation

Female-group

in this group we will enroll only female with asthma

Genetic: genetic evaluationOther: MicroRNA evaluation

Interventions

A sampe of blood (3 mL) and of oral mucosa will be take and analyzed using nCounter GX Human Inflammation Kit a comprehensive number of 8SNPs in 3 human genes known to be differentially expressed in allergic asthma.

Female-groupMale-group

Using the sampe of blood (3 mL) using RT-PCR we will evaluate the expression of microRNA in enrolled patients

Female-groupMale-group

Eligibility Criteria

Age6 Years - 75 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

We will perform an observational study in patients with asthma enrolled fro general practitioners and specialist in respiratory disease. After the recruitment a sample of blood and oral mucosa will be taken to evaluate the expression of SNPs and of inflammatory status

You may qualify if:

  • asthma (allergic or not) diagnosed according to Global Initiative for Asthma guidelines (GINA 2020).
  • signed informed consent

You may not qualify if:

  • infectious diseases
  • neurodegenerative diseases
  • autoimmune diseases
  • neoplasms
  • progressive serious medical conditions (such as cancer, AIDS or end-stage renal disease)
  • alcohol consumption (\>3 alcoholic beverages daily)
  • substanceabuse
  • inability to give written informed consent
  • those who will not sign the consent to the processing of personal data

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

AO Materdomini

Catanzaro, Italia, 88100, Italy

RECRUITING

ASP Catanzaro

Catanzaro, 88100, Italy

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

we will take the oral swab in order to evaluate the enzyme expression

MeSH Terms

Conditions

AsthmaHypersensitivityCoitus

Condition Hierarchy (Ancestors)

Bronchial DiseasesRespiratory Tract DiseasesLung Diseases, ObstructiveLung DiseasesRespiratory HypersensitivityHypersensitivity, ImmediateImmune System DiseasesSexual BehaviorBehavior

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
MD PhD

Study Record Dates

First Submitted

September 11, 2020

First Posted

September 28, 2020

Study Start

August 1, 2020

Primary Completion

November 20, 2021

Study Completion

December 30, 2021

Last Updated

November 23, 2020

Record last verified: 2020-11

Data Sharing

IPD Sharing
Will not share

Locations