NCT04514913

Brief Summary

This is a single center study of 60 subjects including those with asthma and mucus plugging, those with asthma and without mucus plugging, and healthy controls. Screening data will be reviewed to determine participant eligibility. Participants who meet all inclusion criteria will participate in image-guided bronchoscopy ("virtual navigation bronchoscopy" \[VNB\]) to sample airway biospecimens in mucus plugged and control airways.

Trial Health

30
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Timeline
Completed

Started Feb 2023

Geographic Reach
1 country

1 active site

Status
withdrawn

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 12, 2020

Completed
5 days until next milestone

First Posted

Study publicly available on registry

August 17, 2020

Completed
2.5 years until next milestone

Study Start

First participant enrolled

February 1, 2023

Completed
8 days until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 9, 2023

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

February 9, 2023

Completed
Last Updated

March 6, 2026

Status Verified

March 1, 2026

Enrollment Period

8 days

First QC Date

August 12, 2020

Last Update Submit

March 5, 2026

Conditions

Keywords

AsthmaMucus PlugBronchoscopyCT Scan

Outcome Measures

Primary Outcomes (2)

  • Difference in eosinophil percentage in broncheoalveolar biospecimens between asthmatic and non-asthmatic airways

    The investigators will examine differences in type-2 inflammatory markers in bronchoalveolar biospecimens. Eosinophils are an indicator of type 2 inflammation in bronchial lavage samples. Indicators of airway inflammation will be compared with healthy control biospecimens and those of non-mucus plugged asthmatics.

    6 months

  • Difference in gene expression of genes that mark IL-13 activation between asthmatic and non-asthmatic airways

    Genes such as periostin, CLCA1, and Serpin B2 will be analyzed for expression in epithelial cell brushings.

    6 months

Study Arms (3)

Healthy Controls

Participants with no history of asthma or other lung diseases.

Radiation: Multidetector Computed Tomography (MDCT) ScanProcedure: Virtual Navigation Bronchoscopy (VNB)

Asthmatics without mucus plugs

Participants with asthma and no evidence of mucus plugging.

Radiation: Multidetector Computed Tomography (MDCT) ScanProcedure: Virtual Navigation Bronchoscopy (VNB)

Asthmatics with mucus plugs

Participants with asthma and evidence by MDCT lung scan showing mucus plugging.

Radiation: Multidetector Computed Tomography (MDCT) ScanProcedure: Virtual Navigation Bronchoscopy (VNB)

Interventions

This is a low-dose CT scan of the chest using no contract material.

Asthmatics with mucus plugsAsthmatics without mucus plugsHealthy Controls

This is a procedure where a camera will be inserted into the lung in order to guide sample collection of bronchoalveolar lavages, bronchail brushes and endobronchial biopsies.

Asthmatics with mucus plugsAsthmatics without mucus plugsHealthy Controls

Eligibility Criteria

Age18 Years - 70 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Participants with asthma who present with or without mucus plugs, as well as healthy controls, all between the ages of 18 and 70.

You may qualify if:

  • Male or female between the ages of 18 and 70 years at Visit 1 and at least 50% whose age is \> 40 years (to ensure age balance among groups and anticipating that group 3 subjects will be older).
  • Written informed consent obtained from subject and ability for subject to comply with the requirements of the study.
  • Able to perform reproducible spirometry according to ATS criteria.

You may not qualify if:

  • Upper respiratory tract infection (URI) within the previous 6 weeks.
  • History of lung disease.
  • Smoking of tobacco or other recreational inhalants in last month and/or \>10 pack-year smoking history.
  • Currently pregnant.
  • History of allergic rhinitis.
  • Laboratory evidence of atopy (blood eosinophils \> 0.4 cells/dL, IgE \> 165 IU/mL)
  • History of unstable cardiovascular disease.
  • BMI \> 45
  • Currently taking anticoagulants.
  • Group 2: Asthmatic Subjects without Mucus Plugs
  • Male or female between the ages of 18 and 70 years at Visit 1 and at least 50% whose age is \> 40 years (to ensure age balance among groups and anticipating that group 3 subjects will be older).
  • Written informed consent obtained from subject and ability for subject to comply with the requirements of the study.
  • Able to perform reproducible spirometry according to ATS criteria.
  • Physiological evidence of airflow obstruction (FEV1 bronchodilator reversibility of ≥ 12% or hyperreactivity to methacholine reflected by a methacholine PC20 ≤ 16 mg/mL). Historical methacholine data from previous studies conducted in the past 5 years at the UCSF ACRC will be allowed.
  • Clinical history of asthma per patient report or medical record.
  • +24 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

UCSF Airway Clinical Research Center

San Francisco, California, 94143, United States

Location

Related Links

Biospecimen

Retention: SAMPLES WITH DNA

bronchoalveolar lavage from airways in 1-2 bronchopulmonary segments, bronchial brushes from airways in 2-3 bronchopulmonary segments, endobronchial biopsies from airways in 2-3 bronchopulmonary segments, and blood.

MeSH Terms

Conditions

Asthma

Condition Hierarchy (Ancestors)

Bronchial DiseasesRespiratory Tract DiseasesLung Diseases, ObstructiveLung DiseasesRespiratory HypersensitivityHypersensitivity, ImmediateHypersensitivityImmune System Diseases

Study Officials

  • John Fahy, MD, MSc

    University of California, San Francisco

    PRINCIPAL INVESTIGATOR
0

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
CROSS SECTIONAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

August 12, 2020

First Posted

August 17, 2020

Study Start

February 1, 2023

Primary Completion

February 9, 2023

Study Completion

February 9, 2023

Last Updated

March 6, 2026

Record last verified: 2026-03

Data Sharing

IPD Sharing
Will not share

There is no plan to make IPD available to other researchers.

Locations