NCT04455204

Brief Summary

The aim of the present study is to evaluate the implication of adipokines, inflammation, insulin resistance and endothelial dysfunction in the pathogenesis of preeclampsia and in pregnancy related complications.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
60

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Apr 2016

Typical duration for all trials

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

April 25, 2016

Completed
3.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 30, 2019

Completed
1 day until next milestone

Study Completion

Last participant's last visit for all outcomes

October 1, 2019

Completed
9 months until next milestone

First Submitted

Initial submission to the registry

June 29, 2020

Completed
3 days until next milestone

First Posted

Study publicly available on registry

July 2, 2020

Completed
Last Updated

July 7, 2020

Status Verified

July 1, 2020

Enrollment Period

3.4 years

First QC Date

June 29, 2020

Last Update Submit

July 2, 2020

Conditions

Outcome Measures

Primary Outcomes (6)

  • Interleukin 18

    IL-18 will be carried out using Enzyme-Linked Immunosorbent Assay

    Nine months

  • Vaspin level

    Serum vaspin concentrations will be assayed using commercially available ELISA kits

    Nine months

  • resistin level

    Serum resistin concentrations will be assayed using commercially available ELISA kits

    Nine months

  • Malondialdehyde level

    MDA will be measured spectrophotometrically

    Nine Months

  • Serum asymmetteric dimethyl arginine (ADMA)

    Serum asymmetteric dimethyl arginine (ADMA) will be done using Enzyme-Linked Immunosorbent Assay

    Nine Months

  • insulin resistance (IR)

    insulin resistance (IR) using the HOMA-IR index \[18\], which is defined as fasting insulin (μIU/ml) times fasting glucose (mmol/l) divided by 22.5 or divided by 405 if fasting blood glucose is expressed in mass units (mg/dl).

    Nine Months

Study Arms (3)

non-pregnant women (group 1)

20 non-pregnant women who serve as a control group (group 1)

Diagnostic Test: Serum asymmetteric dimethyl arginineDiagnostic Test: serum lipid profilesDiagnostic Test: Serum vaspinDiagnostic Test: Serum resistin

pregnant women (group 2)

20 pregnant women with normal pregnancy at their third trimesters (group 2)

Diagnostic Test: Serum asymmetteric dimethyl arginineDiagnostic Test: serum lipid profilesDiagnostic Test: Serum vaspinDiagnostic Test: Serum resistin

pregnant women with Preeclampsia (group 3)

20 pregnant women with Preeclampsia in their third trimester (group 3) will be screened to fit the inclusion and exclusion criteria.

Diagnostic Test: Serum asymmetteric dimethyl arginineDiagnostic Test: serum lipid profilesDiagnostic Test: Serum vaspinDiagnostic Test: Serum resistin

Interventions

Serum asymmetteric dimethyl arginine (ADMA) will be done using Enzyme-Linked Immunosorbent Assay

Also known as: ADMA
non-pregnant women (group 1)pregnant women (group 2)pregnant women with Preeclampsia (group 3)
serum lipid profilesDIAGNOSTIC_TEST

serum lipid profiles including triglycerides (TG), total cholesterol and high-density lipoprotein cholesterol (HDL-C) which will be measured by enzymatic colorimetric method) using commercial kits

Also known as: Lipids
non-pregnant women (group 1)pregnant women (group 2)pregnant women with Preeclampsia (group 3)
Serum vaspinDIAGNOSTIC_TEST

vaspin concentrations will be assayed using commercially available ELISA kits

non-pregnant women (group 1)pregnant women (group 2)pregnant women with Preeclampsia (group 3)
Serum resistinDIAGNOSTIC_TEST

resistin concentrations will be assayed using commercially available ELISA kits

non-pregnant women (group 1)pregnant women (group 2)pregnant women with Preeclampsia (group 3)

Eligibility Criteria

Age25 Years - 35 Years
Sexfemale
Age GroupsAdult (18-64)
Sampling MethodProbability Sample
Study Population

total number of 60 women who will be further classified into: 20 non-pregnant women who serve as control group (group 1), 20 pregnant women with normal pregnancy at their third trimesters (group 2) and 20 pregnant women with Preeclampsia at their third trimester (group 3) will be screened to fit the inclusion and exclusion criteria. The pregnant women will be assessed in their third trimesters and further re-evaluated 4 weeks after delivery. The gestational ages will be determined conventionally and re-affirmed by ultrasonographic measurement.

You may qualify if:

  • Pregnant women with normal pregnancy (n=20)
  • Pregnant women with Preeclampsia (n=20) at their third trimesters of pregnancy will be included in the study. Preeclampsia will be defined and diagnosed by constant elevation in blood pressure greater than 140/90 mmHg and by the presence of proteinuria above 300 mg /24 hour with the absence of both urinary tract infection and previous history of hypertension.
  • Apparently healthy non-pregnant females (n=20) will be included in this study also.

You may not qualify if:

  • Women with risk factors for oxidative stress such as smokers and
  • Those with a medical history of diabetes mellitus (DM) and Tuberculosis (T.B).
  • Women with a history of hypertension, familial hyperlipidemia, liver diseases, inflammatory diseases, and renal impairment.
  • Non-pregnant women on hormonal therapy or hormonal contraceptives.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (2)

  • Jia X, Wang S, Ma N, Li X, Guo L, Liu X, Dong T, Liu Y, Lu Q. Comparative analysis of vaspin in pregnant women with and without gestational diabetes mellitus and healthy non-pregnant women. Endocrine. 2015 Mar;48(2):533-40. doi: 10.1007/s12020-014-0318-7. Epub 2014 Jun 15.

    PMID: 24929806BACKGROUND
  • Jahromi AS, Shojaei M, Ghobadifar MA. Insulin resistance and serum levels of interleukin-17 and interleukin-18 in normal pregnancy. Immune Netw. 2014 Jun;14(3):149-55. doi: 10.4110/in.2014.14.3.149. Epub 2014 Jun 19.

    PMID: 24999311BACKGROUND

MeSH Terms

Conditions

Pre-Eclampsia

Condition Hierarchy (Ancestors)

Hypertension, Pregnancy-InducedPregnancy ComplicationsFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital Diseases

Study Officials

  • Tarek M Mostafa, Ass. Prof.

    Tanta University

    STUDY DIRECTOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

June 29, 2020

First Posted

July 2, 2020

Study Start

April 25, 2016

Primary Completion

September 30, 2019

Study Completion

October 1, 2019

Last Updated

July 7, 2020

Record last verified: 2020-07

Data Sharing

IPD Sharing
Will not share