Investigation of the Serum Mucoprotein 3 (MUC3) Protein Levels in Gestational Hypertensive Disease

NCT04421183 | Completed

• 1 other identifier: MUC 3
• Study Type: observational
• Target: 80
• Locations: 1 country
• Sites: 1

Brief Summary

Hypertensive diseases of pregnancy are one of the most frequently encountered reasons for maternal and neonatal morbidity and mortality. The excretion of high levels of mucoprotein takes part in the pathogenesis of preeclampsia through restraining trophoblast invasion. This study aimed to investigate levels of serum mucoprotein three in hypertensive diseases of pregnancy. This study included a total of eighty cases that 20 normal pregnancy, 20 gestational hypertension, 20 preeclampsia, and 20 HELLP syndrome patients from June 2010- January 2011.

Conditions

Muc3; Pregnancy Induced Hypertension

Keywords

Muc3; Preeclampsia; Eclampsia; HELLP

Outcome Measures

Primary Outcomes (2)

• Collection of Blood Samples

  The blood samples required for the study were obtained from samples taken into a heparinized tube. Maternal blood samples were taken at the time of diagnosis in patients with preeclamptic - eclamptic, gestational hypertension and HELLP syndrome before starting any treatment when they apply for delivery in normal pregnant women. After the blood samples were centrifuged at Eppendorf Centrifuge-5084R at 3000 G for 10 minutes, the serum was separated. Some of the serum collected were placed in a 1.5 cc Eppendorf tube and stored at -80 ˚C in the medical cooler (Ariston) in the Department of Obstetrics and Gynecology.

  6 months

• Measurement of Mucoprotein in Serum

  After collecting all serum samples (80 pieces), they were dissolved at room temperature and MUC3 (Human Mucin 3, MUC3 ELISA Kit) level was measured in serum samples with the help of commercial ELISA kits. The measurement technique principle is given below.Mucoprotein content in serum was determined in ng/ml with the help of a special ELISA kit system and elements for mucoprotein 3.

  1 month

Study Arms (4)

1

preeclampsia

Diagnostic Test: draw blood

4

normal pregnancy without complication

Diagnostic Test: draw blood

2

hellp

Diagnostic Test: draw blood

3

eclampsia

Diagnostic Test: draw blood

Interventions

draw blood DIAGNOSTIC_TEST

draw blood from the all of patients

1; 2; 3; 4

Eligibility Criteria

• Age: 18 Years - 45 Years
• Gender Eligibility Details: pregnancy is female-specific. therefore, women were included in the study.
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

patients who were diagnosed with hypertensive disease among the patients who applied to the pregnant clinic of Karadeniz Technical University Faculty of Medicine, Obstetrics and Gynecology Department with the approval of the KTÜ Faculty of Medicine Research Ethics Committee dated 27.05.2010 and 154 number ( 80 cases, including the study group and 20 normotensive pregnant control group) were included in the study between June 2010 and January 2011.

You may qualify if:

• To accept the consent to participate in the research and sign the form,
• According to ACOG 2002, pregnancy meets the criteria of hypertensive diseases \[1\], The diagnosis of HELLP syndrome was made according to the criteria described by Sibai. These; abnormal peripheral smear, schistocyte in the peripheral smear, lactate dehydrogenase \> 600 U / L, hemolysis, total bilirubin\> 1.2 mg / dl, AST\> 70 U / L, platelet count \<100,000mm3.
• When the demographic characteristics and CVs of all cases were questioned in the initial evaluation, not having chronic inflammation or acute infection, not having any systemic disease, recurrent pregnancy loss, thrombophilia, antiphospholipid antibody syndrome,
• Not smoking, No history of cancer, No multiple pregnancies
• Not using antihypertensive, anticoagulant, NSAID, corticosteroid or antiplatelet and antiepileptic drugs

You may not qualify if:

• using antihypertensive, anticoagulant, NSAID, corticosteroid or antiplatelet and antiepileptic drugs
• smoking,history of cancer, multiple pregnancies

Sponsors & Collaborators

• Karadeniz Technical University: lead

Study Sites (1)

Karadeniz Technique University

Trabzon, Ortahisar, 61080, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Hypertension, Pregnancy-Induced; Pre-Eclampsia; Eclampsia

Condition Hierarchy (Ancestors)

Pregnancy Complications; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Hypertension; Vascular Diseases; Cardiovascular Diseases

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Target Duration: 2 Days
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: professor

Study Record Dates

• First Submitted: June 2, 2020
• First Posted: June 9, 2020
• Study Start: June 1, 2010
• Primary Completion: January 1, 2011
• Study Completion: January 2, 2011
• Last Updated: June 9, 2020

Record last verified: 2020-06

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)