NCT04386226

Brief Summary

This study evaluates the impact of two Ambrotose products on immunity, gut health, and associated measures in healthy men and women. Subjects are randomly assigned in double-blind manner to one of five conditions: 1) 2 grams Advanced Ambrotose, 2) 4 grams Advanced Ambrotose, 3) 2 grams Ambrotose LIFE, 4) 4 grams Ambrotose LIFE, or 5) placebo. Subjects ingested their assigned condition daily for eight weeks.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
75

participants targeted

Target at P75+ for not_applicable healthy

Timeline
Completed

Started Aug 2018

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

August 15, 2018

Completed
7 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 15, 2019

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2019

Completed
11 months until next milestone

First Submitted

Initial submission to the registry

May 5, 2020

Completed
8 days until next milestone

First Posted

Study publicly available on registry

May 13, 2020

Completed
Last Updated

April 28, 2021

Status Verified

April 1, 2021

Enrollment Period

7 months

First QC Date

May 5, 2020

Last Update Submit

April 26, 2021

Conditions

Keywords

AmbrotoseImmunityGut Health

Outcome Measures

Primary Outcomes (39)

  • general well-being (Short Form-12)

    A 12-item questionnaire was used to measure functional health and well-being from the subject's point of view during their intervention. Scores range from 0 to 100, with higher scores representing better self-reported health

    at baseline

  • general well-being (Short Form-12)

    A 12-item questionnaire was used to measure functional health and well-being from the subject's point of view during their intervention. Scores range from 0 to 100, with higher scores representing better self-reported health

    at 4 weeks

  • general well-being (Short Form-12)

    A 12-item questionnaire was used to measure functional health and well-being from the subject's point of view during their intervention. Scores range from 0 to 100, with higher scores representing better self-reported health

    at 8 weeks

  • Self-reported psychological general well-being index

    The Self-reported psychological general well-being index was used to assess subject's perceived well-being during their intervention. The index goes from 0 (worst possible level of well-being) to 110 (maximum level of well being)

    at baseline

  • The Self-reported psychological general well-being index was used to assess subject's perceived well-being during their intervention. The index goes from 0 (worst possible level of well-being) to 110 (maximum level of well being)

    The Self-reported psychological general well-being index was used to assess subject's perceived well-being during their intervention. The index goes from 0 (worst possible level of well-being) to 110 (maximum level of well being)

    at 4 weeks

  • The Self-reported psychological general well-being index was used to assess subject's perceived well-being during their intervention. The index goes from 0 (worst possible level of well-being) to 110 (maximum level of well being)

    The Self-reported psychological general well-being index was used to assess subject's perceived well-being during their intervention. The index goes from 0 (worst possible level of well-being) to 110 (maximum level of well being)

    at 8 weeks

  • Self-reported assessment of fatigue & associated variables

    A visual analog scale in which the subject was asked to make a mark on a 100-mm scale to indicate how he/she felt in regards to the variable in question during their intervention with 0 being "not at all" and 100 being "the most"

    at baseline

  • Self-reported assessment of fatigue & associated variables

    A visual analog scale in which the subject was asked to make a mark on a 100-mm scale to indicate how he/she felt in regards to the variable in question during their intervention with 0 being "not at all" and 100 being "the most"

    at 4 weeks

  • Self-reported assessment of fatigue & associated variables

    A visual analog scale in which the subject was asked to make a mark on a 100-mm scale to indicate how he/she felt in regards to the variable in question during their intervention with 0 being "not at all" and 100 being "the most"

    at 8 weeks

  • White blood cell numbers

    We determined the white blood cell numbers for each subject during their intervention.

    at baseline

  • White blood cell numbers

    We determined the white blood cell numbers for each subject during their intervention.

    at 4 weeks

  • White blood cell numbers

    We determined the white blood cell numbers for each subject during their intervention.

    at 8 weeks

  • Interleukin-10 (IL-10) level following blood incubation

    IL-10 was measured following blood incubation with Roswell Park Memorial Institute (RPMI) medium for 6 hrs

    at baseline

  • Interleukin-10 (IL-10) level following blood incubation

    IL-10 was measured following blood incubation with Roswell Park Memorial Institute (RPMI) medium for 6 hrs

    at 4 weeks

  • Interleukin-10 (IL-10) level following blood incubation

    IL-10 was measured following blood incubation with Roswell Park Memorial Institute (RPMI) medium for 6 hrs

    at 8 weeks

  • Interleukin-6 (IL-6) level following blood incubation

    IL-6 was measured following blood incubation with RPMI medium for 6 hrs

    at baseline

  • Interleukin-6 (IL-6) level following blood incubation

    IL-6 was measured following blood incubation with RPMI medium for 6 hrs

    at 4 weeks

  • Interleukin-6 (IL-6) level following blood incubation

    IL-6 was measured following blood incubation with RPMI medium for 6 hrs

    at 8 weeks

  • Interleukin-1beta (IL-1beta) level following blood incubation

    IL-1beta was measured following blood incubation with RPMI medium for 6 hrs

    at baseline

  • Interleukin-1beta (IL-1beta) level following blood incubation

    IL-1beta was measured following blood incubation with RPMI medium for 6 hrs

    at 4 weeks

  • Interleukin-1beta (IL-1beta) level following blood incubation

    IL-1beta was measured following blood incubation with RPMI medium for 6 hrs

    at 8 weeks

  • Tumor Necrosis Factor alpha (TNFalpha) level following blood incubation

    TNFalpha was measured following blood incubation with RPMI medium for 6 hrs

    at baseline

  • Tumor Necrosis Factor alpha (TNFalpha) level following blood incubation

    TNFalpha was measured following blood incubation with RPMI medium for 6 hrs

    at 4 weeks

  • Tumor Necrosis Factor alpha (TNFalpha) level following blood incubation

    TNFalpha was measured following blood incubation with RPMI medium for 6 hrs

    at 8 weeks

  • IL-10 level following blood incubation with lipopolysaccharide (LPS)

    IL-10 was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at baseline

  • IL-10 level following blood incubation with lipopolysaccharide (LPS)

    IL-10 was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 4 weeks

  • IL-10 level following blood incubation with lipopolysaccharide (LPS)

    IL-10 was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 8 weeks

  • IL-6 level following blood incubation with LPS

    IL-6 was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at baseline

  • IL-6 level following blood incubation with LPS

    IL-6 was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 4 weeks

  • IL-6 level following blood incubation with LPS

    IL-6 was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 8 weeks

  • IL-1beta level following blood incubation with LPS

    IL-1beta was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at baseline

  • IL-1beta level following blood incubation with LPS

    IL-1beta was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 4 weeks

  • IL-1beta level following blood incubation with LPS

    IL-1beta was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 8 weeks

  • TNFalpha level following blood incubation with LPS

    TNFalpha was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at baseline

  • TNFalpha level following blood incubation with LPS

    TNFalpha was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 4 weeks

  • TNFalpha level following blood incubation with LPS

    TNFalpha was measured following blood incubation with RPMI medium containing lipopolysaccharide (final concentration 250 ng/mL) for 6 hrs

    at 8 weeks

  • Serum Zonulin levels

    Zonulin from blood serum during the intervention was measured using an ELISA

    at baseline

  • Serum Zonulin levels

    Zonulin from blood serum during the intervention was measured using an ELISA

    at 4 weeks

  • Serum Zonulin levels

    Zonulin from blood serum during the intervention was measured using an ELISA

    at 8 weeks

Secondary Outcomes (3)

  • Dietary Intake

    at baseline

  • Dietary Intake

    at 4 weeks

  • Dietary Intake

    at 8 weeks

Study Arms (5)

2 grams Ambrotose LIFE

EXPERIMENTAL

2 grams daily for 8 weeks

Dietary Supplement: Ambrotose LIFE

4 grams Ambrotose LIFE

EXPERIMENTAL

4 grams daily for 8 weeks

Dietary Supplement: Ambrotose LIFE

2 grams Advanced Ambrotose

ACTIVE COMPARATOR

2 grams daily for 8 weeks

Dietary Supplement: Advanced Ambrotose

4 grams Advanced Ambrotose

ACTIVE COMPARATOR

4 grams daily for 8 weeks

Dietary Supplement: Advanced Ambrotose

Placebo

PLACEBO COMPARATOR

4 grams Maltodextrin daily for 8 weeks

Other: Placebo

Interventions

Ambrotose LIFEDIETARY_SUPPLEMENT

Ambrotose LIFE contains aloe vera extract inner leaf gel, arabinogalactin, ghatti gum, glucosamine hydrogen chloride, gum tragacanth, vitamin A, beta carotene, wakame algae extract, rice starch, RiFiber (rice bran), and Modified Citrus Pectin with Sodium Alginate.

2 grams Ambrotose LIFE4 grams Ambrotose LIFE
Advanced AmbrotoseDIETARY_SUPPLEMENT

Advanced Ambrotose contains aloe vera extract inner leaf gel, arabinogalactan, ghatti gum, glucosamine hydrogen chloride, gum tragacanth, vitamin A, beta carotene, wakame algae extract, and rice starch

2 grams Advanced Ambrotose4 grams Advanced Ambrotose
PlaceboOTHER

Maltodextrin

Placebo

Eligibility Criteria

Age20 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • must be physically active by participating in structured exercise at least twice per week for 30 or more minutes per session;
  • not be pregnant

You may not qualify if:

  • diagnosed cardiovascular disease
  • diagnosed metabolic disease
  • diagnosed neurological disease
  • using nutritional supplements or medications known to impact immunity or gut health

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Center for Nutraceutical and Dietary Supplement Research

Memphis, Tennessee, 38152, United States

Location

Study Officials

  • Richard Bloomer, PhD

    University of Memphis

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Masking Details
Double-Blind
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Model Details: Double-Blind, Randomized, Control
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Dean

Study Record Dates

First Submitted

May 5, 2020

First Posted

May 13, 2020

Study Start

August 15, 2018

Primary Completion

March 15, 2019

Study Completion

June 1, 2019

Last Updated

April 28, 2021

Record last verified: 2021-04

Data Sharing

IPD Sharing
Will not share

Locations