Metabolomic Impacts of Branched-chain Amino Acid Supplementation During Endurance Exercise

NCT04969536 | Completed

• 1 other identifier: DF081319
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The overall goal of this investigation was to determine the impact of branch-chained amino acids (BCAAs) supplementation on serotonin and associated metabolites during endurance exercise on healthy individuals. Specifically, despite the strong theoretical basis for supplementation of BCAAs to attenuate serotonin production and fatigue during exercise, most human clinical trials have failed to demonstrate these benefits. To shed light on this discrepancy, the purpose of this study was to measure the impact of BCAA supplementation on serotonin and associated metabolites during exercise. To achieve this purpose, investigators determined the effects of the consumption of a BCAA beverage on endurance exercise performance and serum metabolites. The supplementation protocol consisted of two trials in which each participant consumed 8 grams of BCAA supplement in a beverage or a placebo in randomized order. It was hypothesized that post-exercise metabolites would differ between BCAA and placebo conditions.

Conditions

Healthy

Keywords

Branch-chained amino acids; Endurance exercise; Metabolomics; Serotonin

Outcome Measures

Primary Outcomes (2)

• Serotonin

  Serum serotonin concentration

  60 minutes

• Untargeted metabolomics

  Liquid chromatography mass spectrometry (LCMS) analysis of serum metabolites

  60 minutes

Secondary Outcomes (1)

• Rating of perceived exertion Borg Scale

  60 minutes

Study Arms (2)

Branched chain amino acid supplementation

EXPERIMENTAL

Consumption of 8 ounces of water with addition of 2.5 g of leucine, 1.25 g of isoleucine, and 1.25 g of valine immediately prior to and again 30 minutes into a 60 minute treadmill run at 65% of VO2max.

Dietary Supplement: Branched Chain Amino Acids

Placebo

PLACEBO COMPARATOR

Consumption of 8 ounces of a flavor matched beverage sweetened with a sucralose-based drink mix immediately prior to and again 30 minutes into a 60 minute treadmill run at 65% of VO2max.

Other: Placebo

Interventions

Branched Chain Amino Acids DIETARY_SUPPLEMENT

Supplementation with 8 g Nutrex Research Amino Acid Drive BCAA in 8 ounces of water once each immediately pre-exercise and after 30 minutes of exercise

Branched chain amino acid supplementation

Placebo OTHER

Supplementation with 2.0 milliliters of a sucralose-based drink mix with 8 ounces of water once each immediately pre-exercise and after 30 minutes of exercise

Placebo

Eligibility Criteria

• Age: 18 Years - 55 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Participate in at least 5 hours of cardiovascular activity per week

You may not qualify if:

• Any underlying injury
• The consumption of any selective serotonin re-uptake inhibitor (SSRI) medication

Sponsors & Collaborators

• Montana State University: lead
• M.J. Murdoch Charitable Trust: collaborator
• Kreighbaum Endowment: collaborator

Study Sites (1)

Montana State University

Bozeman, Montana, 59717, United States

Location

MeSH Terms

Interventions

Amino Acids

Intervention Hierarchy (Ancestors)

Amino Acids, Peptides, and Proteins

Study Officials

• Mary P Miles, PhD

  Montana State University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Masking Details: Double-blind
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: 60 minutes of moderate (65% of VO2max) endurance exercise with either a branched chain amino acid supplement or a placebo consumed immediately prior to and 30 minutes into the exercise bout.

Study Record Dates

• First Submitted: July 9, 2021
• First Posted: July 20, 2021
• Study Start: October 1, 2019
• Primary Completion: February 23, 2021
• Study Completion: February 23, 2021
• Last Updated: May 3, 2023

Record last verified: 2021-07

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)