To Establish a Reproducible Organoid Culture Model With Human Kidney Cancer

NCT04342286 | Completed

• 1 other identifier: CRE-2019.211
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Kidney cancer is one of the ten most common malignancies, and the incidence is increasing in recent year. From Hong Kong Cancer Registry, there was about 670 new cases diagnosed in 2016, and had been increased by 46% compared to 2007.Within the broad classification of kidney cancers, renal cell carcinoma (RCC) accounts for approximately 85% of all cases and greater than 90% of all renal malignancies. Despite the improved understanding and also diagnosis for kidney cancer, still about one fourth of patients will presented at metastatic stage or developed recurrence after initial treatment and required further systemic therapy. Facing the wide range of available options for systemic therapy, the current challenge is how to select the most effective treatment. Unfortunately, there is no good biomarkers available to aid treatment selection. Currently, there are some approaches to try to test the response of kidney cancer to different chemotherapeutic agents. Previous studies showed that 3D organoid culture model can improve our ability to model tumor behavior. Organoid culture technology may provide opportunities for new drug development and drug screening. In this study, investigators aim to establish a reliable and effective method to cultivate kidney cancer cells, then will provide researchers for further information on personalized and targeted therapy on kidney cancer for local Hong Kong patients.

Conditions

Kidney Cancer

Outcome Measures

Primary Outcomes (1)

• To establish a sustainable human kidney tumor 3D Matrigel culture system with a stable phenotype

  A Cell culture is successful when organoids grow from dividing cells

  2 years

Secondary Outcomes (2)

• The identicality of histopathological detail and genomic information of the kidney cancer organoid compared with the original primary tissue

  2 years

• The tumorigenicity ability of the kidney cancer organoid in nude mice

  2 years

Interventions

Organoid culture GENETIC

Organoid culture

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

This is a methodology development project aiming at developing a reproducible 3D kidney cancer organoid model. Twenty patients suffered from renal cell carcinoma and planned for surgical treatment will be recruited for this study.

You may qualify if:

• Male patients \> 18 years old
• Patients suffered from renal cell carcinoma require surgical removal of kidney

You may not qualify if:

• Patients fail to provide informed consent
• The collection of tissue will affect the pathological interpretation of the specimen

Sponsors & Collaborators

• Chinese University of Hong Kong: lead

Study Sites (1)

Prince of Wales Hospital

Shatin, Hong Kong

Location

Related Publications (14)

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Related Links

• Hong Kong Cancer Registry

Biospecimen

Retention: SAMPLES WITH DNA

Blood and Urine sample

MeSH Terms

Conditions

Kidney Neoplasms

Condition Hierarchy (Ancestors)

Urologic Neoplasms; Urogenital Neoplasms; Neoplasms by Site; Neoplasms; Female Urogenital Diseases; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Kidney Diseases; Urologic Diseases; Male Urogenital Diseases

Study Officials

• Chi Fai NG, MD

  Chinese University of Hong Kong

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: April 8, 2020
• First Posted: April 13, 2020
• Study Start: May 1, 2020
• Primary Completion: February 22, 2021
• Study Completion: February 22, 2021
• Last Updated: December 16, 2021

Record last verified: 2021-12

Data Sharing

• IPD Sharing: Will not share

Locations

HK (1)