Effect of the Type and Emulsification of Dietary Fat on the Carotenoids Absorption

NCT04323826 | Completed

• 1 other identifier: S10
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 2

Brief Summary

This study is designed to assess the effects of 1) the emulsification 2) the type of dietary fat on carotenoid (CAT) absorption employing an in-vitro digestion system (Proof of Concept) and well-designed randomized controlled trial.

Conditions

Carotenoids

Keywords

Carotenoids absorption; blood triglycerides; postprandial lipid

Outcome Measures

Primary Outcomes (2)

• Blood carotenoids concentrations

  Five main carotenoids compounds, lutein, zeaxanthin, α-carotene, β-carotene and lycopene concentrations will be determined in blood via HPLC.

  Up to 6 months

• Blood triglycerides concentrations

  Plasma triglyceride concentration will be determined by using a Cobas c311 chemistry analyzer (Roche Diagnostics, Asia Pacific).

  Up to 6 months

Secondary Outcomes (5)

• Skin carotenoid status

  Up to 4 months

• Height

  Baseline data, up to 4 weeks

• Weight

  Baseline data, up to 4 weeks

• Waist circumference

  Baseline data, up to 4 weeks

• Diet

  Up to 6 months

Study Arms (4)

salads with olive oil-water mixture

EXPERIMENTAL

Vegetable salads (100g tomatoes, 62g carrots, 70g spinach, 25g lettuce and 5g wolfberry) with 56g olive oil-water mixture will be provided to consume.

Other: olive oil-water mixture

salads with olive oil-water emulsion

EXPERIMENTAL

Vegetable salads (100g tomatoes, 62g carrots, 70g spinach, 25g lettuce and 5g wolfberry) with 56g olive oil-water emulsion (4% whey protein isolate as emulsifier) will be provided to consume.

Other: olive oil-water emulsion

salads with coconut oil-water emulsion

EXPERIMENTAL

Vegetable salads (100g tomatoes, 62g carrots, 70g spinach, 25g lettuce and 5g wolfberry) with 56g coconut oil-water emulsion (4% whey protein isolate as emulsifier) will be provided to consume.

Other: coconut oil-water emulsion

salads with coconut oil-water mixture

EXPERIMENTAL

Vegetable salads (100g tomatoes, 62g carrots, 70g spinach, 25g lettuce and 5g wolfberry) with 56g coconut oil-water mixture will be provided to consume.

Other: coconut oil-water mixture

Interventions

olive oil-water mixture OTHER

50% olive oil in water, processed with high shear mixer at 8000 rpm power without adding emulsifier, well shaked before consumption.

salads with olive oil-water mixture

olive oil-water emulsion OTHER

50% olive oil in water (4% whey protein isolate), stirred for 5 mins using a high shear mixer at 8000 rpm power and was further processed with high pressure homogenizer at 100 MPa.

salads with olive oil-water emulsion

coconut oil-water mixture OTHER

50% coconut oil in water, processed with high shear mixer at 8000 rpm power without adding emulsifier, well shaked before consumption.

salads with coconut oil-water mixture

coconut oil-water emulsion OTHER

50% coconut oil in water (4% whey protein isolate), stirred for 5 mins using a high shear mixer at 8000 rpm power and was further processed with high pressure homogenizer at 100 MPa.

salads with coconut oil-water emulsion

Eligibility Criteria

• Age: 19 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Ability to give an informed consent
• Age 19 to 45 years
• Willing to follow the study procedures

You may not qualify if:

• Significant weight change \> 3 kg in the past 3 months;
• Exercising vigorously over the past 3 months (\*Defined as having \> 6 metabolic equivalents of exercise daily; approximately 20 mins of moderate intensity exercise (e.g. slow jogging);
• Intestinal disorders including lipid malabsorption;
• Diabetes or prediabetes (fasting blood glucose \> 100 mg/dL);
• Smoking;
• Drinking more than 2 alcoholic drinks per day;
• Taking lipid-lowering medications or dietary supplements affecting plasma lipid-lipoprotein concentration.
• Menopausal women, those using hormone based contraceptives, those having abnormal menstrual cycles, and those who are pregnant, lactating or planning to become pregnant.
• Following any restricted diet (e.g. vegetarian)
• Taking dietary supplements which may impact the outcome of interests (e.g. carotenoids supp.)
• Insufficient venous access to allow the blood collection

Sponsors & Collaborators

• National University of Singapore: lead
• Singapore Institute of Food and Biotechnology Innovation: collaborator

Study Sites (2)

Department of Food Science and Technology; National University of Singapore

Singapore, 117546, Singapore

Location

Investigational Medical Unit (IMU)

Singapore, 117599, Singapore

Location

Related Publications (2)

• Kim JE, Gordon SL, Ferruzzi MG, Campbell WW. Effects of egg consumption on carotenoid absorption from co-consumed, raw vegetables. Am J Clin Nutr. 2015 Jul;102(1):75-83. doi: 10.3945/ajcn.115.111062. Epub 2015 May 27.

  PMID: 26016861 BACKGROUND

• Yao Y, Yang Z, Yin B, Goh HM, Toh DWK, Kim JE. Effects of dietary fat type and emulsification on carotenoid absorption: a randomized crossover trial. Am J Clin Nutr. 2023 May;117(5):1017-1025. doi: 10.1016/j.ajcnut.2023.03.011. Epub 2023 Mar 13.

  PMID: 36921903 DERIVED

Study Officials

• KIM Jung EUN

  National University of Singapore

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: SUPPORTIVE CARE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professor

Study Record Dates

• First Submitted: March 24, 2020
• First Posted: March 27, 2020
• Study Start: April 1, 2021
• Primary Completion: August 31, 2021
• Study Completion: September 1, 2021
• Last Updated: September 10, 2021

Record last verified: 2021-09

Data Sharing

• IPD Sharing: Will not share

Locations

SG (2)