Effect of Exercise on the Human Skeletal Muscle Phosphoproteome

NCT04263714 | Completed Results

• 1 other identifier: 2196
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

Generally, resistance exercise increases muscle mass and strength, and fatigue resistance. How resistance exercise achieves these adaptations remains understudied, but what is known is that skeletal muscle translates the physical and biochemical stresses of resistance exercise into morphological and metabolic adaptations. While resistance exercise activates signaling pathways (i.e., proteins) that increase the synthesis of specific proteins to cause adaptations, thousands of proteins are likely involved, and their interactions are complicated. The investigators aim to study these processes.

Conditions

Skeletal Muscle Protein Synthesis

Outcome Measures

Primary Outcomes (1)

• Change in Acute Muscle Protein Synthesis

  Myofibrillar and intracellular enrichments of L-\[ring- 13C6\] phenylalanine will be measured. MPS will be calculated using the precursor-product equation: MPS =(\[E2b-E1b\]/\[Eic x t\]) x 100. Eb represents the enrichment of bound myofibrillar protein, Eic is the average intracellular enrichment between two biopsies, and t is the tracer incorporation time in h. As we will employ 'tracer naïve' participants (had not previously participated in a study protocol where L-\[ring- 13C6\] phenylalanine was infused), a pre-infusion blood sample will be used for the calculation of resting myofibrillar MPS. The outcomes measure will be expressed as a percentage change of MPS relative to the baseline measure. i.e. intracellular enrichment of L-\[ring- 13C6\] phenylalanine relative to the baseline (resting) period.

  Pre exercise (resting) to 3 hours post-exercise

Study Arms (1)

Exercise

EXPERIMENTAL

All subjects will perform both aerobic and resistance exercise

Behavioral: Exercise

Interventions

Exercise BEHAVIORAL

Aerobic exercise and resistance exercise

Exercise

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• \- between the ages of 18 and 30 years

You may not qualify if:

• Smoker or user of tobacco products;
• High physical activity
• Have health problems such as: renal or gastrointestinal disorders, metabolic disease, heart disease, vascular disease, rheumatoid arthritis, diabetes, poor lung function, uncontrolled blood pressure, dizziness, thyroid problems, or any other health conditions for which you are being treated that might put you at risk for this study;
• Taking anti-diabetic, anti-inflammatory, platelet inhibitor, or anti-coagulant medications;
• Use of an investigational drug product within the last 30 days;
• Have participated in an infusion protocol in the last year; or
• Do not understand English or have a condition the PI believes would interfere with a participants' ability to provide informed consent, comply with the study protocol, or which might confound the interpretation of the study results or put someone at undue risk.

Sponsors & Collaborators

• McMaster University: lead

Study Sites (1)

Exercise Metabolism Research Laboratory, McMaster Univeristy

Hamilton, Ontario, L8S 4K1, Canada

Location

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Results Point of Contact

• Title: Dr. Stuart Phillips
• Organization: McMaster University

phillis@mcmaster.ca

9055259140

Study Officials

• Stuart Phillips, PhD

  McMaster University, Department of Kinesiology

  PRINCIPAL INVESTIGATOR

Publication Agreements

• PI is Sponsor Employee: No
• Restrictive Agreement: No

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: Aerobic and resistance exercise

Study Record Dates

• First Submitted: February 5, 2020
• First Posted: February 11, 2020
• Study Start: April 1, 2020
• Primary Completion: July 1, 2023
• Study Completion: May 1, 2024
• Last Updated: November 21, 2024
• Results First Posted: November 21, 2024

Record last verified: 2024-11

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, CSR
• Time Frame: At the time of publication
• Access Criteria: Everyone can access

Locations

CA (1)