Pilot Study-Quantitative Analysis of Positional Fatty Acids in Postprandial Chylomicron Triacylglycerol Using 13C NMR Spectroscopy

NCT04156503 | Completed

• 1 other identifier: PD222/17
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

13C NMR spectroscopy has been validated to be a rapid, direct and accurate quantitative method in determination of positional distribution of fatty acids in edible oils and fats, including the palm oil fractions (from soft olein to hard stearin). Nevertheless, there is paucity of literature describing its application in determining the positional fatty acid composition in blood triacylglycerols \& biological samples, despite its widely used application in edible oils and fats. The postprandial feeding intervention is aimed to harvest chylomicron rich in triglycerides (\~97% TAG) for NMR analysis.

Conditions

Healthy

Keywords

Chylomicron; fatty acid composition; lard; palm olein

Outcome Measures

Primary Outcomes (1)

• Triglycerides profile in chylomicron harvested

  the positional composition of fatty acid in test fat that are retained upon absorption ( samples are pooled from 3, 4 \&5 hour after test diet)

  Up to hour 5

Study Arms (2)

Test fat: Palm olein

EXPERIMENTAL

One high fat muffin will be serves together with a glass of low fat milk shake.

Other: Palm olein vs Lard

Test fat: Lard

EXPERIMENTAL

One high fat muffin will be serves together with a glass of low fat milk shake.

Other: Palm olein vs Lard

Interventions

Palm olein vs Lard OTHER

A 5-hour postprandial feeding intervention with 2-weeks washout in between. Blood samples will be collected at hour 0 (baseline) before test meal, and hour 3, 4 and 5 after consumption of test meal.

Test fat: Lard; Test fat: Palm olein

Eligibility Criteria

• Age: 25 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Non muslim, aged between 25 - 40 yrs old.
• BMI between 20 - 27.5 kg/m2.
• Blood pressure with \<130mmHg for systolic pressure and \< 80 mmHg for diastolic pressure.
• Blood glucose level in the range of 3.9 to 5.5 mmol/L.
• Total cholesterol level \< 6.0 mmol/L.
• Triglycerides level \< 2.3 mmol/L.
• No use of tobacco and no consumption of alcohol.

You may not qualify if:

• Suffer from chronic disease such as CVD, hypertension, diabetic, metabolic syndrome.
• Consuming any medical prescriptions/ supplements.
• Having blood clotting problem.
• Women who are pregnant or lactating.
• Hypertensive condition with systolic pressure \>140mmHg and diastolic pressure \> 90 mmHg.

Sponsors & Collaborators

• Malaysia Palm Oil Board: lead

Study Sites (1)

MalaysiaPOB

Kajang, Selangor, 43000, Malaysia

Location

Study Officials

• Phooi Tee Voon, Ph.D

  MPOB

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER GOV
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 4, 2019
• First Posted: November 7, 2019
• Study Start: October 18, 2019
• Primary Completion: December 30, 2019
• Study Completion: December 31, 2020
• Last Updated: February 27, 2026

Record last verified: 2026-02

Data Sharing

• IPD Sharing: Will not share

Locations

MY (1)