Culture System for Isolating Circulating Tumor cells_2

NCT04125667 | Unknown

• 1 other identifier: 2019071239RINB
• Study Type: observational
• Target: 45
• Locations: 1 country
• Sites: 1

Brief Summary

This culture system utilizes the special affinity difference of biomedical material coating for different cells to achieve the effect of isolating tumor cells from the blood sample. The coating of the system has the characteristic that to make the WBCs adhesion, but the cancer cells in the blood sample suspend in the culture medium, which achieves the effect of separating cancer cells from the blood. The supernatant with the cancer cells can further be isolated from the cultural system for related analysis and detection to achieve early diagnosis and screening.

Conditions

Early Diagnosis; Diagnostic Techniques and Procedures

Keywords

Circulating cancer cells; Biomedical polymer material; Cell affinity difference; White blood cell attachment

Outcome Measures

Primary Outcomes (1)

• The Number of Circulating Cancer Cells from The Blood Sample

  The number of circulating cancer cells from the blood sample will be investigated. To distinguish cancer cells from blood cells for the system, the lung cancer cells were immunofluorescence stained for Cytokeratin, white blood cells were stained for CD45 and nuclei were counterstained with DAPI.

  about 1 week

Secondary Outcomes (1)

• Cancer Cell Isolation Efficiency of The Isolation System

  about 1 week

Eligibility Criteria

• Age: 20 Years - 70 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The study population will include all stages of lung cancer patients between the ages of 20 and 70. Also, to protect the vulnerable subjects, we don't recruit minors, prisoners, aborigines, pregnant women, people with mental disorders, students, and subordinates, etc.

You may qualify if:

• Patients with lung cancer are between the ages of 20 and 70
• Willing to sign the consent form under the informed consent

You may not qualify if:

• Vulnerable Subjects included minors, prisoners, aborigines, pregnant women, people with mental disorders, students, and subordinates, etc.

Sponsors & Collaborators

• National Taiwan University Hospital: lead

Study Sites (1)

National Taiwan University Hospital

Taipei, Taiwan

RECRUITING

MeSH Terms

Conditions

Disease

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• Tai-Horng Young

  Institute of Biomedical Engineering, National Taiwan University

  STUDY CHAIR

Central Study Contacts

Tai-Horng Young

CONTACT

886-2-23123456; thyoung@ntu.edu.tw

Yin-Tzu Chen

CONTACT

886-2-23123456; R07528032@ntu.edu.tw

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: October 2, 2019
• First Posted: October 14, 2019
• Study Start: October 31, 2019
• Primary Completion: August 31, 2020
• Study Completion: August 31, 2020
• Last Updated: October 14, 2019

Record last verified: 2019-09

Locations

TW (1)