Can Amino Acid Supplementation Augment the Anabolic Response in Tendon After Exercise

NCT04067479 | Completed

• 1 other identifier: 1704019133
• Study Type: interventional
• Target: 7
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this study is to determine if an oral provision of amino acids will enhance exercise-induced increases in markers of Achilles tendon collagen production.

Conditions

Tendinopathy

Keywords

tendon; amino acid; exercise

Outcome Measures

Primary Outcomes (2)

• Achilles peritendinous concentrations of pro-collagen

  Peritendinous samples will be collected using microdialysis. Pro-collagen will be assessed using enzyme immunoassay kits

  0-6 hours post consumption

• Amino Acid Levels

  Achilles peritendinous amino acid concentrations will be assess using high performance liquid chromatography.

  0-6 hours post consumption

Study Arms (2)

Young

EXPERIMENTAL

21-30 year old men and women Inclusion/Exclusion Criteria 1. All subjects will be sedentary or recreationally active (sedentary: one day or less per week of aerobic or resistance exercise for at least a year or recreationally active: not training for competitive events). 2. Age: 21-30 years 3. Body mass index \<35 kg•m-2, 4. Chronic users of medications such as acetaminophen, ibuprofen, or prescription cyclooxygenase inhibitors will be excluded as these medications have been shown to alter tendon physiology. 5. Individuals with diabetes will be excluded. Diabetes is thought to change the structure of tendon. 6. Individuals with a history of tendon pain will be excluded, as chronic tendon pain is associated with changes in the structure of tendon. 7. Persons with allergies/sentivitiy to amino acids or ingedianet of Cystal Light will be excluded.

Dietary Supplement: Amino Acids

Older Adults

EXPERIMENTAL

Inclusion/Exclusion Criteria 1. All subjects will be sedentary or recreationally active (sedentary: one day or less per week of aerobic or resistance exercise for at least a year or recreationally active: not training for competitive events). 2. Age: 60-75 years 3. Body mass index \<35 kg•m-2 4. Chronic users of medications such as acetaminophen, ibuprofen, or prescription cyclooxygenase inhibitors will be excluded as these medications have been shown to alter tendon physiology. 5. Individuals with diabetes will be excluded. Diabetes is thought to change the structure of tendon. 6. Individuals with a history of tendon pain will be excluded, as chronic tendon pain is associated with changes in the structure of tendon. 7. Persons with allergies/sentivitiy to amino acids or ingedianet of Cystal Light will be excluded.

Dietary Supplement: Amino Acids

Interventions

Amino Acids DIETARY_SUPPLEMENT

Oral bolus of essential amino acids

Older Adults; Young

Eligibility Criteria

• Age: 21 Years - 80 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• years or 60-80 years old

You may not qualify if:

• diabetes
• BMI\>35
• acute illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, metabolic disorders, arthritis, or a history of neuromuscular problems.
• chronic pain medication users
• alcoholism
• competitive athletes
• pregnant women will be excluded
• Individuals with specific allergies to amino acids

Sponsors & Collaborators

• Purdue University: lead

Study Sites (1)

Purdue University

West Lafayette, Indiana, 47907, United States

Location

MeSH Terms

Conditions

Tendinopathy; Motor Activity

Interventions

Amino Acids

Condition Hierarchy (Ancestors)

Muscular Diseases; Musculoskeletal Diseases; Tendon Injuries; Wounds and Injuries; Behavior

Intervention Hierarchy (Ancestors)

Amino Acids, Peptides, and Proteins

Study Officials

• Chad C Carroll, PhD

  Purdue University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: August 20, 2019
• First Posted: August 26, 2019
• Study Start: May 9, 2017
• Primary Completion: December 13, 2022
• Study Completion: December 20, 2022
• Last Updated: April 11, 2023

Record last verified: 2023-04

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)