NCT04022863

Brief Summary

Rationale: Cancer is primarily diagnosed by clinical presentation, imaging and pathological analysis of tissue biopsies, increasingly supported by molecular diagnostics tests. However, late diagnosis and misdiagnosis due to limitations of tissue biopsy acquisition remains a major problem. Therefore, a general blood test to pinpoint cancer early and adequately can be considered the 'Holy Grail', because diagnosis in an earlier stage significantly improves the chance of cure from cancer. Several blood-based sources are currently being evaluated as liquid biopsies, including circulating tumor (ct) DNA and circulating tumor cells, but none of these have been implemented for primary (multiclass) cancer diagnostics. Protein tumor markers have been used for decades in diagnosis and monitoring of treatment response in different cancers. Tumor-educated platelets (TEPs) can function as potential blood-based source for (early) cancer diagnostics. Blood platelets are implicated in hemostasis and wound healing. Platelets have recently emerged as central players and immediate responders in the systemic and local responses to tumor growth. Confrontation of platelets by tumor cells via transfer of tumor-associated molecules ('education') results in the sequestration of these molecules (derived from both tumor and its micro-environment), causing a distinct platelet messenger Ribonucleic acid (mRNA) profile. We have previously shown that platelets acquire glioblastoma and prostate cancer mRNA biomarkers and that glioblastoma TEP mRNA profiles harbour diagnostic potential. Furthermore, circulating tumor desoxyrubonucleic acid (ctDNA) has recently been implicated as biomarker for therapy effectiveness and survival. Objective: develop and evaluate the potential of combination of tumor markers, TEPs and ctDNA as liquid biomarkers for (early) ovarium cancer diagnostics and as markers for therapy response and survival. Study design: investigator-initiated, longitudinal, observational study. Study population: patients suspected of having ovarium cancer and are therefore planned for surgery. Main study parameters/endpoints: The difference in biomarker profile from benign ovarium lesions versus cancerous lesions. Nature and extent of the burden and risks associated with participation, benefit and group relatedness. There is no extra burden/risk for the patients in this study. Three extra vials of blood.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
500

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Jul 2019

Longer than P75 for all trials

Geographic Reach
1 country

3 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

July 1, 2019

Completed
8 days until next milestone

First Submitted

Initial submission to the registry

July 9, 2019

Completed
8 days until next milestone

First Posted

Study publicly available on registry

July 17, 2019

Completed
4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2023

Completed
5 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2023

Completed
Last Updated

July 17, 2019

Status Verified

July 1, 2019

Enrollment Period

4 years

First QC Date

July 9, 2019

Last Update Submit

July 14, 2019

Conditions

Keywords

TEPctDNAovarian cancer

Outcome Measures

Primary Outcomes (3)

  • accuracy of TEP's to determine the nature of an ovarian tumor

    Prior to operation blood will be drawn of patients with an ovarian tumor. TEP's will be analysed as described before.

    1 month

  • accuracy of ctDNA to determine the nature of an ovarian tumor

    Prior to operation blood will be drawn of patients with an ovarian tumor. CtDNA will be analysed as described before.

    1 month

  • accuracy of ctDNA to predict treatment response in ovarian cancer

    Prior to debulking operation and pre and post chemotherapy ctDNA will be analysed in blood from patients with ovarian cancer as described before.

    1 month

Study Arms (3)

ovarian tumor benign

all pathological proven benign ovarian tumors

Diagnostic Test: TEPDiagnostic Test: ctDNA

ovarian tumor borderline

all pathological proven borderline ovarian tumors

Diagnostic Test: TEPDiagnostic Test: ctDNA

ovarian tumor malignant

all pathological proven malignant ovarian tumors

Diagnostic Test: TEPDiagnostic Test: ctDNA

Interventions

TEPDIAGNOSTIC_TEST

Tumor Educated Platelets

ovarian tumor benignovarian tumor borderlineovarian tumor malignant
ctDNADIAGNOSTIC_TEST

circulating tumor DNA

ovarian tumor benignovarian tumor borderlineovarian tumor malignant

Eligibility Criteria

Age18 Years - 90 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility Detailswomen with ovarian tumors
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

all women presenting with an ovarian tumor of unknown nature

You may qualify if:

  • Suspicion of ovarian cancer.

You may not qualify if:

  • Previous intraabdominal malignancies in the history

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

Catharina hospital

Eindhoven, North Brabant, 5623EJ, Netherlands

RECRUITING

Netherlands Cancer Institute

Amsterdam, North Holland, 1066 CX, Netherlands

RECRUITING

Leiden University Medical Center

Leiden, North Holland, 2333 ZA, Netherlands

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Tumor educated platelets circulating tumor DNA

MeSH Terms

Conditions

Ovarian Neoplasms

Condition Hierarchy (Ancestors)

Endocrine Gland NeoplasmsNeoplasms by SiteNeoplasmsOvarian DiseasesAdnexal DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital Neoplasms, FemaleUrogenital NeoplasmsGenital DiseasesEndocrine System DiseasesGonadal Disorders

Study Officials

  • Thomas Wurdinger, PhD

    Amsterdam UMC loc VUmc

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Target Duration
1 Month
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
principal investigator

Study Record Dates

First Submitted

July 9, 2019

First Posted

July 17, 2019

Study Start

July 1, 2019

Primary Completion

July 1, 2023

Study Completion

December 1, 2023

Last Updated

July 17, 2019

Record last verified: 2019-07

Locations