NCT03960333

Brief Summary

This is a study to learn about obesity and how insulin resistance and Type 2 Diabetes develops in children.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
82

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Apr 2019

Longer than P75 for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

April 25, 2019

Completed
11 days until next milestone

First Submitted

Initial submission to the registry

May 6, 2019

Completed
17 days until next milestone

First Posted

Study publicly available on registry

May 23, 2019

Completed
1.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 5, 2021

Completed
3.4 years until next milestone

Study Completion

Last participant's last visit for all outcomes

June 30, 2024

Completed
Last Updated

February 13, 2025

Status Verified

February 1, 2025

Enrollment Period

1.8 years

First QC Date

May 6, 2019

Last Update Submit

February 11, 2025

Conditions

Keywords

ObesityType2 Diabetes MellitusType2 DiabetesInsulin ResistanceMetforminPediatric Health

Outcome Measures

Primary Outcomes (2)

  • circulating CD4+T cells in Overweight/Obese vs Lean

    We will measure and report the percent of ATP derived from glycolysis and oxidative phosphorylation in circulating CD4+ T cells as well as the % CD4+CD25+CD127lowFoxP3+ cells (Tregs) in overweight/obese vs lean children.

    After completion of all study visits, approximately 2 years.

  • circulating CD4+T cells in Type 2 Diabetic pre/post Metformin

    We will measure and report the percent of ATP derived from glycolysis and oxidative phosphorylation in circulating CD4+ T cells as well as the % CD4+CD25+CD127lowFoxP3+ cells (Tregs) in Type 2 Diabetic children pre and post-Metformin treatment.

    After completion of all study visits, approximately 2 years.

Study Arms (3)

Healthy Lean

Healthy lean individuals (n=20) defined with a Body Mass Index (BMI) ≥ 5th percentile and \<85th percentile for age/sex will be recruited. Participants in this cohort will be asked to complete a one-time study visit.

Overweight/Obese

Overweight/Obese individuals (n=20) defined with a Body Mass Index (BMI) ≥ 85th percentile for age/sex will be recruited. Participants in this cohort will be asked to complete a one-time study visit.

Type 2 Diabetes or Insulin Resistant

Obese individuals with Type 2 Diabetes or insulin resistance who have been recently prescribed Metformin and have a Body Mass Index (BMI) ≥ 85th percentile for age/sex (n=20) will be recruited. Participants in this cohort will be asked to complete a two study visits approximately 6 months apart.

Eligibility Criteria

Age5 Years - 17 Years
Sexall
Age GroupsChild (0-17)
Sampling MethodNon-Probability Sample
Study Population

110 children ages 5-17 years old will be recruited with the goal that approximately 80 subjects stratified across the following groups will complete the study: i) healthy lean (approximately n=20); ii) overweight/obese (approximately n=40, with the anticipation that approximately 20 of these children will be insulin resistant); iii) overweight/obese with T2D and prescribed Metformin (approximately n=20). Every attempt will be made to ensure that the desired number for each group is achieved and groups are balanced with respect to age, sex and ethnicity.

You may qualify if:

  • Age 5-17 years, inclusive
  • Either healthy lean (BMI≥ 5th percentile and \<85th percentile for age/sex) or overweight (BMI ≥ 85th percentile and \<95th) or obese (BMI ≥ 95th percentile for age/sex)
  • For those with BMI≥ 85th percentile for age/sex, parental verbal confirmation that the child had a history of BMI≥ 85th percentile for age/sex for at least six months prior to study enrollment OR
  • Age 5 years - 17 years 5 months, inclusive
  • Either overweight or obese (BMI≥ 85th percentile for age/sex)
  • Parental verbal confirmation that the child had a history of BMI≥ 85th percentile for age/sex for at least six months prior to study enrollment
  • Diagnosed with type 2 diabetes mellitus or insulin resistance
  • Prescribed metformin (either not yet taking or began taking within 3 weeks of enrollment)

You may not qualify if:

  • Having an infection (viral, respiratory, gastrointestinal) in the previous 4 weeks
  • Genetic or physical conditions impacting mobility over past year as determined by the PI
  • Having known chronic illnesses/disorders that may independently affect study outcome measures: type 1 diabetes mellitus, neurologic (e.g. epilepsy), developmental (developmental delay, autism spectrum disorder), endocrine (thyroid, Cushing's), hepatic, autoimmune, cardiac and renal disorders. Also, chronic lung disorders except well controlled asthma that does not require permanent use of inhaled/oral steroids
  • Taking any of the following medications that can affect study outcome: antipsychotics, thyroid hormone replacement therapy, inhaled/oral steroids, insulin, anabolic drugs (growth hormone replacement therapy and oxandrolone) and stimulants
  • Taking metformin prescribed as part of their clinical care for longer than 3 weeks at the time of enrollment (may begin metformin therapy prescribed as part of their clinical care while enrolled in the study)
  • BMI\<5th percentile for age/sex (classified as underweight based on CDC growth charts)
  • Subjects determined ineligible by the PI or delegated staff.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Arkansas Children's Research Institute

Little Rock, Arkansas, 72202, United States

Location

Related Publications (1)

  • Rose S, Landes RD, Vyas KK, Delhey L, Blossom S. Regulatory T cells and bioenergetics of peripheral blood mononuclear cells linked to pediatric obesity. Immunometabolism (Cobham). 2024 Apr 25;6(2):e00040. doi: 10.1097/IN9.0000000000000040. eCollection 2024 Apr.

Biospecimen

Retention: SAMPLES WITHOUT DNA

Blood, urine, and stool samples may be retained. The blood sample will be used for bioenergetics, immunophenotyping, CD4+ T cell isolations, metabolic control pathway analysis, quantification of plasma CRP, pro-inflammatory, and anti-inflammatory cytokines and adipokines, and analyte analysis. The sample may be used for future research studies on pediatric nutrition. The urine and stool sample will be used for future research studies on pediatric nutrition.

MeSH Terms

Conditions

ObesityDiabetes Mellitus, Type 2Insulin Resistance

Condition Hierarchy (Ancestors)

OverweightOvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsDiabetes MellitusGlucose Metabolism DisordersMetabolic DiseasesEndocrine System DiseasesHyperinsulinism

Study Officials

  • Shannon Rose, PhD

    Arkansas Children's Research Institute

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
OTHER
Time Perspective
CROSS SECTIONAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 6, 2019

First Posted

May 23, 2019

Study Start

April 25, 2019

Primary Completion

February 5, 2021

Study Completion

June 30, 2024

Last Updated

February 13, 2025

Record last verified: 2025-02

Data Sharing

IPD Sharing
Will share

The information collected at the study visit, urine, and stool samples, and remaining blood samples may be stored indefinitely and may be used for future research studies on pediatric nutrition or metabolism. Prior to the information collected at the study visit and samples being used for future research studies, the PI will assess the ethics and scientific merit of the proposed research with the samples, and proposed future research will be reviewed by the IRB as may be required.

Shared Documents
CSR
Time Frame
The data and samples will be available after all data has been collected for the study and all samples have been processed for the study. Prior to the information collected at the study visit and samples being used for future research studies, the PI will assess the ethics and scientific merit of the proposed research with the samples, and proposed future research will be reviewed by the Institutional Review Board (IRB) as may be required.
Access Criteria
The samples and health information collected for the study visit may be shared with researchers at the University of Arkansas for Medical Sciences, Arkansas Children's Hospital, or Arkansas Children's Research Institute. The samples may be shared with an outside group. The samples will only have a study number, visit number, and study acronym to maintain confidentiality.

Locations