Correlation Between Autophagy-related Proteins and Inflammatory Markers in Hemodialysis Patients

NCT03925753 | Completed DMC

• 1 other identifier: 107054
• Study Type: observational
• Target: 62
• Locations: 1 country
• Sites: 1

Brief Summary

One recent study demonstrated impaired autophagy in patients receiving hemodialysis (HD). To clarify whether this alteration is related to the inflammatory state in HD patients, we focused on basal autophagy in peripheral blood mononuclear cells (PBMCs) of HD patients with and without inflammation and controls. PBMCs were harvested using Ficoll density gradient centrifugation . Levels of the autophagy-associated proteins ubiquitin-binding protein p62 (p62), microtubule-associated proteins 1A/1B light chain 3A (LC3I/II) and beclin-1 in PBMCs will be detected by western blotting. Enzyme-linked immunosorbent assay kits will be used to detect the serum concentrations of interleukin (IL)-6, liposaccharide-binding protein (LBP) and tumor necrosis factor (TNF)-α.

Conditions

Autophagy

Keywords

autophagy; inflammatory markers

Outcome Measures

Primary Outcomes (3)

• Levels of the autophagy-associated proteins ubiquitin-binding protein p62 (p62)

  ubiquitin-binding protein p62 (p62) in PBMCs will be detected by western blotting.

  1 years

• Levels of the autophagy-associated proteins microtubule-associated proteins 1A/1B light chain 3A (LC3I/II)

  microtubule-associated proteins 1A/1B light in PBMCs will be detected by western blotting.

  1 years

• Levels of the autophagy-associated proteins beclin-1

  beclin-1 in PBMCs will be detected by western blotting.

  1 years

Secondary Outcomes (3)

• Analysis of biomarkers of Liposaccharide-binding protein (LBP)

  1 years

• Analysis of biomarkers of IL-6

  1 years

• Measurements of TNF-α

  1 years

Eligibility Criteria

• Age: 20 Years - 90 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

patients have to be at least 20-years-old and on outpatient hemodialysis (HD) for at least 3 months.

You may qualify if:

• Both sexes aged between 20-90 years.
• Received stable hemodialysis at least 3 months.
• Written informed consent.

You may not qualify if:

• patients with severe infections, severe heart disease and liver disease, malignancy, autoimmune disorders, severe malnutrition, or clinical conditions requiring oral nutrition supplements;
• Inability to follow protocol.
• Pregnancy or wishing/trying to get pregnant

Sponsors & Collaborators

• Tungs' Taichung Metroharbour Hospital: lead

Study Sites (1)

Tungs' Taichung MetroHarbour Hospital

Taichung, Taiwan

Location

Study Officials

• Paik Seong Lim, PhD

  Tungs' Taichung Metroharbour Hospital

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 22, 2019
• First Posted: April 24, 2019
• Study Start: March 1, 2019
• Primary Completion: December 31, 2019
• Study Completion: December 31, 2019
• Last Updated: February 1, 2021

Record last verified: 2021-01

Locations

TW (1)