Molecular and Hormonal Responses to Diet-Induced Insulin Resistance

NCT03879187 | Completed

• 1 other identifier: R13-P171
• Study Type: interventional
• Target: 15
• Locations: 0 countries
• Sites: N/A

Brief Summary

This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.

Conditions

Healthy

Keywords

High-fat diet; Insulin; Glucose; Muscle; eNOS; Isotopes

Outcome Measures

Primary Outcomes (5)

• Change in glucose concentration

  Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L.

  Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

• Change in insulin concentration

  Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L.

  Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

• Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers

  Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min.

  Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

• Change in skeletal muscle insulin signalling

  AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units.

  Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

• Change in markers of microvascular function

  endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.

  Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

Secondary Outcomes (1)

• Change intramuscular triglyceride content

  Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)

Study Arms (1)

High-fat diet

EXPERIMENTAL

Participants underwent a 7 day high-fat, high-energy diet intervention with metabolic measurements before and after

Dietary Supplement: High-fat, high-energy diet

Interventions

High-fat, high-energy diet DIETARY_SUPPLEMENT

Participants consumed a diet that was high in fat and high in energy

High-fat diet

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Aged between 18 and 40 years
• Physically active
• Non-smoker
• Not taking any medication known to interfere with the study
• No history of cardiovascular disease or diabetes
• Weight stable for the last 6 months

You may not qualify if:

• Outside of age range
• Sedentary
• Smoker
• Taking medication known to interfere with the study
• History of cardiovascular disease or diabetes
• Unstable weight within last 6 months

Sponsors & Collaborators

• Loughborough University: lead

Related Publications (1)

• Parry SA, Turner MC, Woods RM, James LJ, Ferguson RA, Cocks M, Whytock KL, Strauss JA, Shepherd SO, Wagenmakers AJM, van Hall G, Hulston CJ. High-Fat Overfeeding Impairs Peripheral Glucose Metabolism and Muscle Microvascular eNOS Ser1177 Phosphorylation. J Clin Endocrinol Metab. 2020 Jan 1;105(1):dgz018. doi: 10.1210/clinem/dgz018.

  PMID: 31513265 DERIVED

MeSH Terms

Conditions

Insulin Resistance

Condition Hierarchy (Ancestors)

Hyperinsulinism; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Senior Lecturer; Principal Investigator

Model Details: 15 healthy individuals were assessed before and after consuming a high-fat, high-energy diet for 7 days.

Study Record Dates

• First Submitted: March 12, 2019
• First Posted: March 18, 2019
• Study Start: March 2, 2015
• Primary Completion: May 22, 2018
• Study Completion: May 22, 2018
• Last Updated: March 18, 2019

Record last verified: 2019-03

Data Sharing

• IPD Sharing: Will not share