NCT03819829

Brief Summary

This protocol will be used to collect crucial components of the immune system from healthy volunteers for the characterization of immune cells in fresh blood. A pipeline has been set up for comprehensive immune phenotyping of both lymphoid and myeloid cells within 24 hours after blood withdrawal.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
50

participants targeted

Target at P50-P75 for not_applicable healthy-volunteers

Timeline
18mo left

Started Feb 2019

Longer than P75 for not_applicable healthy-volunteers

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress83%
Feb 2019Dec 2027

First Submitted

Initial submission to the registry

December 13, 2018

Completed
2 months until next milestone

First Posted

Study publicly available on registry

January 29, 2019

Completed
7 days until next milestone

Study Start

First participant enrolled

February 5, 2019

Completed
7.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 2, 2026

Expected
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

December 2, 2027

Last Updated

March 23, 2023

Status Verified

March 1, 2023

Enrollment Period

7.8 years

First QC Date

December 13, 2018

Last Update Submit

March 21, 2023

Conditions

Outcome Measures

Primary Outcomes (1)

  • The number of immune cells, both lymphoid and myeloid, present in the blood of healthy individuals, assessed by flow cytometry, as well as cytokine expression and transcriptome profiles of these cells

    the number of lymphoid and myeloid immune cells present in the blood of healthy individuals using flow cytometry, assessing the cytokine expression of these cells in healthy individuals using ELISA cytokine assays, and assessing gene expression of these cells using single cell transcriptomics analyses using droplet-based single cell RNA sequencing.

    assessed up to 12 months

Secondary Outcomes (1)

  • Immunophenotyping of both myeloid and lymphoid cells in healthy individuals

    assessed up to 12 months

Study Arms (1)

Blood sample

OTHER

Blood sample

Other: Blood sample

Interventions

Blood sample

Blood sample

Eligibility Criteria

Age36 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Age above 35 years
  • Written informed consent
  • No history of cancer
  • No use of systemic immunosuppressive medication (eg. corticosteroids). Local use of corticosteroids (eg. topical or inhalation) is allowed.

You may not qualify if:

  • Fever 14 days before blood withdrawal
  • Donated blood for the same program within the last 2 years
  • Pregnancy

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

NKI-AVL

Amsterdam, 1066CX, Netherlands

RECRUITING

MeSH Terms

Interventions

Blood Specimen Collection

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative Techniques

Study Officials

  • M Kok, MD

    NKI-AvL

    PRINCIPAL INVESTIGATOR

Central Study Contacts

M Kok, MD

CONTACT

L Voorwerk, MD

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 13, 2018

First Posted

January 29, 2019

Study Start

February 5, 2019

Primary Completion (Estimated)

December 2, 2026

Study Completion (Estimated)

December 2, 2027

Last Updated

March 23, 2023

Record last verified: 2023-03

Locations