Fasting and Exercise-induced Changes in Human Skeletal Muscle
Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle
1 other identifier
interventional
10
1 country
1
Brief Summary
This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for not_applicable
Started Jan 2019
Shorter than P25 for not_applicable
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
January 14, 2019
CompletedFirst Submitted
Initial submission to the registry
January 17, 2019
CompletedFirst Posted
Study publicly available on registry
January 22, 2019
CompletedPrimary Completion
Last participant's last visit for primary outcome
April 30, 2019
CompletedStudy Completion
Last participant's last visit for all outcomes
April 30, 2019
CompletedMay 30, 2019
May 1, 2019
4 months
January 17, 2019
May 29, 2019
Conditions
Outcome Measures
Primary Outcomes (1)
Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
PGC-1α gene expression (indirect indicator of PGC-1α activity)
15 min before fast; after 4 and 8 hours of fasting
Secondary Outcomes (20)
Catecholamine concentrations
15 min before fast; after exercise; after 4 and 8 hours of fasting
Glucose and insulin concentrations
15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated AMP activated protein kinase (AMPK)
15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated SIRT-1
15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated mammalian target of rapamycin (mTOR)
15 min before fast; after 4 and 8 hours of fasting
- +15 more secondary outcomes
Study Arms (2)
FAST+EX - FAST
EXPERIMENTALFAST+EX then FAST alone
FAST - FAST+EX
EXPERIMENTALFAST alone then FAST+EX
Interventions
Eligibility Criteria
You may qualify if:
- recreationally active (no more than 3 hours of weekly physical activity)
- not currently involved in a systematic training program
- body mass index \< 30 kg/m2
You may not qualify if:
- presence of cardiometabolic disease
- taking regular oral medication
- current smoker
Contact the study team to confirm eligibility.
Sponsors & Collaborators
- Queen's Universitylead
- University of Waterloocollaborator
Study Sites (1)
School of Kinesiology and Health Studies
Kingston, Ontario, K7L3N6, Canada
Study Officials
- PRINCIPAL INVESTIGATOR
Brendon J. Gurd, PhD
Queen's University
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- RANDOMIZED
- Masking
- DOUBLE
- Who Masked
- INVESTIGATOR, OUTCOMES ASSESSOR
- Masking Details
- Allocation concealment, assessor blinding
- Purpose
- BASIC SCIENCE
- Intervention Model
- CROSSOVER
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Associate Professor
Study Record Dates
First Submitted
January 17, 2019
First Posted
January 22, 2019
Study Start
January 14, 2019
Primary Completion
April 30, 2019
Study Completion
April 30, 2019
Last Updated
May 30, 2019
Record last verified: 2019-05
Data Sharing
- IPD Sharing
- Will not share