Combating Dengue With Innovative, Paradigm-shift-Strategies: Early Dengue Surveillance in Adult Aedes Mosquitoes

NCT03799237 | Unknown

• 2 other identifiers: GOSNS1-1MO013-2017
• Study Type: interventional
• Target: 7,979
• Locations: 1 country
• Sites: 1

Brief Summary

During dengue outbreaks, the Ministry of Health Malaysia employs various methods to control the spread of disease, including killing the larvae of Aedes mosquitoes, fogging, together with educating and disseminating information about the dengue outbreak, to the community. However, this is too late. Research has shown that when an outbreak has occurred, the viral infection has already spread among the community. Therefore, this current trial aims to educate the public (via questionnaire survey and interactions with the residential managements, mainly), detect dengue-infected mosquitoes, inform the communities of the presence of dengue-infected mosquitoes, followed by approaching and educating them to take precautionary measures before the outbreak happens. Trapping (using gravid oviposition sticky (GOS) traps) and detecting dengue virus non-structural 1 (NS1) antigen (using dengue NS1 kit) in the Aedes mosquitoes will be a more reliable way to alert the community before a potential dengue outbreak in their housing area. The community will receive information of presence of infected mosquitoes and probable dengue infections before dengue cases are reported. This will be an ideal time for clean-ups and for search and destroy activities. With this shift in approach and the use of newer techniques, it is hoped that deaths and epidemics due to dengue will be reduced.

Conditions

Dengue

Keywords

Dengue; Adult Aedes; GOS trap; Dengue NS1 antigen test; Early dengue detection and surveillance

Outcome Measures

Primary Outcomes (2)

• Change in number of weekly notified dengue cases in intervention arm

  The number of notified dengue cases in the study sites will be obtained from the District Health Office

  Assess weekly, through study completion, 1 and a half years

• Change in the duration of dengue outbreaks in intervention arm

  The duration of dengue outbreaks in the study sites will be obtained from the District Health Office.

  Through study completion, an average of 1 and a half years

Secondary Outcomes (2)

• Change in adult Aedes density in the intervention arm

  Assess weekly, through study completion, within 1 and half years.

• Change in level of dengue Knowledge, Attitude and Practice in the intervention arm

  6 months after recruitment for pre-trial questionnaire survey. 3 months after recruitment for post-trial questionnaire survey, through study completion, 1 and a half years

Study Arms (2)

GOS trap and dengue NS1 antigen kit

EXPERIMENTAL

Gravid Oviposition Sticky (GOS) traps will be placed to trap adult Aedes mosquitoes and changed weekly. NS1 will be used to detect dengue in trapped Aedes mosquitoes. When dengue NS1 positive mosquitoes are found, community will be alerted via flyers, banners and other means. Routine Aedes/dengue control and surveillance will be carried out as usual as per the current Ministry of Health guidelines.

Other: GOS trap and dengue NS1 antigen kit

Control

NO INTERVENTION

The GOS traps will be placed randomly in the control arm once per month for entomological survey. Routine Aedes control and surveillance will be carried out as per the current Ministry of Health guidelines. Dengue control measure will be initiated by the health authorities when human cases are reported from this arm.

Interventions

GOS trap and dengue NS1 antigen kit OTHER

The GOS trap lure and sticky surfaces to trap gravid Aedes mosquitoes. Three GOS traps will be placed on every 3 floors of the residential blocks. The traps will be changed weekly. Trapped Aedes mosquitoes will be identified to species. The mosquitoes will then be dissected, to remove the abdomen from the thorax. Five to seven abdomens of the same mosquito species will be pooled and tested for dengue NS1 antigen using the SD Biosensor Standard Q dengue NS1 antigen test kit. If the pool is tested positive for dengue NS1, the head and thorax of the respective abdomens will be subjected to the same procedure for dengue NS1 antigen test, individually. This may allow us to identify a focus where there could be dengue-infected individuals. Flyers/Posters will be disseminated besides, house-to-house approach to inform the residents of the findings and apartment blocks where these mosquitoes were found. The apartment management will also make use of social media disseminate this information.

GOS trap and dengue NS1 antigen kit

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Age 18 years and above, including pregnant women and healthy individuals
• Reside in the study sites
• Work at the study sites for at least 40 hours per week
• Able to give consent
• Willing to participate in blood taking

You may not qualify if:

• Age below 18 years
• Unable to give consent
• Not willing to undergo venepuncture or finger-prick

Sponsors & Collaborators

• Jonathan Liew Wee Kent: lead
• Petaling Jaya City Council, Malaysia: collaborator
• Selangor State Health Department, Malaysia: collaborator

Study Sites (1)

Petaling Jaya City Council

Petaling Jaya, Selangor, Malaysia

RECRUITING

Related Publications (8)

• Lau SM, Chua TH, Sulaiman WY, Joanne S, Lim YA, Sekaran SD, Chinna K, Venugopalan B, Vythilingam I. A new paradigm for Aedes spp. surveillance using gravid ovipositing sticky trap and NS1 antigen test kit. Parasit Vectors. 2017 Mar 21;10(1):151. doi: 10.1186/s13071-017-2091-y.

  PMID: 28327173 BACKGROUND

• Klungthong C, Manasatienkij W, Phonpakobsin T, Chinnawirotpisan P, Rodpradit P, Hussem K, Thaisomboonsuk B, Ong-ajchaowlerd P, Nisalak A, Kalayanarooj S, Buddhari D, Gibbons RV, Jarman RG, Yoon IK, Fernandez S. Monitoring and improving the sensitivity of dengue nested RT-PCR used in longitudinal surveillance in Thailand. J Clin Virol. 2015 Feb;63:25-31. doi: 10.1016/j.jcv.2014.12.009. Epub 2014 Dec 12.

  PMID: 25600599 BACKGROUND

• Roslan MA, Ngui R, Vythilingam I, Sulaiman WYW. Evaluation of sticky traps for adult Aedes mosquitoes in Malaysia: a potential monitoring and surveillance tool for the efficacy of control strategies. J Vector Ecol. 2017 Dec;42(2):298-307. doi: 10.1111/jvec.12270.

  PMID: 29125255 BACKGROUND

• Chadee DD, Ritchie SA. Efficacy of sticky and standard ovitraps for Aedes aegypti in Trinidad, West Indies. J Vector Ecol. 2010 Dec;35(2):395-400. doi: 10.1111/j.1948-7134.2010.00098.x.

  PMID: 21175947 BACKGROUND

• Gama RA, Silva EM, Silva IM, Resende MC, Eiras AE. Evaluation of the sticky MosquiTRAP for detecting Aedes (Stegomyia) aegypti (L.) (Diptera: Culicidae) during the dry season in Belo Horizonte, Minas Gerais, Brazil. Neotrop Entomol. 2007 Mar-Apr;36(2):294-302. doi: 10.1590/s1519-566x2007000200018.

  PMID: 17607465 BACKGROUND

• Lee C, Vythilingam I, Chong CS, Abdul Razak MA, Tan CH, Liew C, Pok KY, Ng LC. Gravitraps for management of dengue clusters in Singapore. Am J Trop Med Hyg. 2013 May;88(5):888-892. doi: 10.4269/ajtmh.12-0329. Epub 2013 Mar 11.

  PMID: 23478581 BACKGROUND

• Lau SM, Vythilingam I, Doss JI, Sekaran SD, Chua TH, Wan Sulaiman WY, Chinna K, Lim YA, Venugopalan B. Surveillance of adult Aedes mosquitoes in Selangor, Malaysia. Trop Med Int Health. 2015 Oct;20(10):1271-80. doi: 10.1111/tmi.12555. Epub 2015 Jul 14.

  PMID: 26094839 BACKGROUND

• Liew JWK, Selvarajoo S, Tan W, Ahmad Zaki R, Vythilingam I. Gravid oviposition sticky trap and dengue non-structural 1 antigen test for early surveillance of dengue in multi-storey dwellings: study protocol of a cluster randomized controlled trial. Infect Dis Poverty. 2019 Sep 3;8(1):71. doi: 10.1186/s40249-019-0584-y.

  PMID: 31477185 DERIVED

MeSH Terms

Conditions

Dengue

Condition Hierarchy (Ancestors)

Mosquito-Borne Diseases; Vector Borne Diseases; Infections; Arbovirus Infections; Virus Diseases; Flavivirus Infections; Flaviviridae Infections; RNA Virus Infections; Hemorrhagic Fevers, Viral

Study Officials

• Jonathan WK Liew, PhD

  University of Malaya

  PRINCIPAL INVESTIGATOR

• Indra Vythilingam, PhD

  University of Malaya

  STUDY DIRECTOR

Central Study Contacts

Jonathan WK Liew, PhD

CONTACT

+60178858375; jonathanliew@um.edu.my

Sivaneswari Selvarajoo, BSc

CONTACT

+60108968645; sivaneswariselvarajoo@gmail.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Postdoctoral Research Fellow

Model Details: Cluster Randomized Controlled Trial

Study Record Dates

• First Submitted: January 2, 2019
• First Posted: January 10, 2019
• Study Start: October 1, 2018
• Primary Completion: March 31, 2020
• Study Completion: April 30, 2020
• Last Updated: January 10, 2019

Record last verified: 2019-01

Data Sharing

• IPD Sharing: Will not share

Locations

MY (1)