Investigation of Hemoglobin Oxygenation With Third Harmonic Generation Microscopy

NCT03619356 | Unknown

• 1 other identifier: 201709032RINB
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

The specific aims of the project are performing a pre-clinical study on a human model and evaluating the oxygenation and other status of human erythrocytes by spectrally-resolved third harmonic generation (srTHG) microscopy . By analyzing the partitioned third harmonic generation (THG) spectra of oxygenated and deoxygenated erythrocytes ex vivo, the investigators aim to provide a reference standard for quantifying oxygenation and molecular status in this project.

Conditions

Hypoxia; Oxygen Deficiency; Tumor; Diabetes

Keywords

hemoglobin; oxygenation concentration; tumor; diabetes

Outcome Measures

Primary Outcomes (3)

• Observe the extent of tumor growth via morphological information

  To observe the extent of tumor growth (with units of mm2) via morphological information. Morphological information are presented as density and shape of red blood cells.

  2 years

• Observe the extent of tumor growth via spectral THG intensity distribution

  To observe the extent of tumor growth (with units of mm2) via spectral THG distributions. The spectral distributions are presented by the ratio between different channels. And the ratio can be calculated by analyzing the excited fluorescence intensity.

  2 years

• Change in spectral THG strength distribution

  Obtain THG strength in 3 different channels for chemically treated whole blood samples for oxygenation, deoxygenation and other status. The spectral distributions are presented by the ratio between different channels.

  2 years

Secondary Outcomes (1)

• Quantify the extent of diseases in spectral THG strength distribution

  1 year

Eligibility Criteria

• Age: 20 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Volunteers with any genders with the age older than 20 years.

You may qualify if:

• older than 20 years old

You may not qualify if:

• Vulnerable groups, such as pregnant women, prisoners, students, mentally handicapped, aborigines, etc.

Sponsors & Collaborators

• National Taiwan University Hospital: lead

Study Sites (1)

National Taiwan University Hospital

Taipei, Taiwan

Location

MeSH Terms

Conditions

Hypoxia; Neoplasms; Diabetes Mellitus

Condition Hierarchy (Ancestors)

Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases; Endocrine System Diseases

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 11, 2018
• First Posted: August 7, 2018
• Study Start: February 5, 2018
• Primary Completion: December 31, 2024
• Study Completion: December 31, 2024
• Last Updated: August 21, 2024

Record last verified: 2023-11

Locations

TW (1)