Gingival Crevicular Fluid Cytokine Levels in Response to Orthodontic Forces

NCT03555747 | Completed

• 1 other identifier: bafacan09
• Study Type: interventional
• Target: 15
• Locations: 0 countries
• Sites: N/A

Brief Summary

The aim of this randomized split-mouth study was to quantify simultaneously 30 analytes including cytokines, chemokines and growth factors in gingival crevicular fluid (GCF) in response to different magnitudes of forces during maxillary canine distalization. The upper right and left canines were distalized using a continuous force of 75 g or 150 g in 15 individuals who had Class II division I malocclusion requiring bilateral extraction of maxillary first premolars. GCF samples were obtained from the tension and the pressure side of each canine at appliance placement and after force application at 24 hours and 28 days. A multiplexed bead immunoassay was used to quantify 30 analytes simultaneously. The effect of force, side and time on the analyte levels were analyzed with Brunner-Langer method.

Conditions

Orthodontic Tooth Movement

Keywords

Canine distalization; Gingival crevicular fluid; Cytokines; Orthodontic force; Periodontium; Gingivitis

Outcome Measures

Primary Outcomes (1)

• Gingival crevicular fluid protein content change

  pg/ml

  Change from baseline to 24 hours and to 28 days after force application

Secondary Outcomes (3)

• Probing pocket depth change

  Change from baseline to 24 hours and to 28 days after force application

• Clinical attachment level change

  Change from baseline to 24 hours and to 28 days after force application

• The amount of tooth movement

  Change from baseline to 28 days after force application

Study Arms (2)

Canine distalization by 75 g force

OTHER

Canine was distalized using a continuous force of 75 g with nickel-titanium closed coil springs.

Other: Canine distalization

Canine distalization by 150 g force

OTHER

Canine was distalized using a continuous force of 150 g with nickel-titanium closed coil springs.

Other: Canine distalization

Interventions

Canine distalization OTHER

GCF was sampled from the mesial (tension) and distal (pressure) sides of maxillary right and left canines immediately before appliance placement (baseline) and after force application (24 hours and 28 days)

Also known as: Gingival crevicular fluid collection

Canine distalization by 150 g force; Canine distalization by 75 g force

Eligibility Criteria

• Age: 11 Years - 16 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)

You may qualify if:

• Systemically healthy
• Non-smoker
• Orthodontic patients with class II division I malocclusion

You may not qualify if:

• Orthodontic treatment history
• Use of anti-inflammatory drugs within one month
• Use of antibiotics within last three months

Sponsors & Collaborators

• Aydin Adnan Menderes University: lead
• Ege University: collaborator
• Karolinska Institutet: collaborator

Related Publications (1)

• Afacan B, Ozturk VO, Gecgelen Cesur M, Kose T, Bostanci N. Effect of orthodontic force magnitude on cytokine networks in gingival crevicular fluid: a longitudinal randomized split-mouth study. Eur J Orthod. 2019 Mar 29;41(2):214-222. doi: 10.1093/ejo/cjy068.

  PMID: 30321318 DERIVED

MeSH Terms

Conditions

Gingivitis

Condition Hierarchy (Ancestors)

Infections; Gingival Diseases; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: INVESTIGATOR
• Purpose: TREATMENT
• Intervention Model: SEQUENTIAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: May 19, 2018
• First Posted: June 13, 2018
• Study Start: May 1, 2015
• Primary Completion: May 29, 2015
• Study Completion: September 30, 2015
• Last Updated: January 14, 2020

Record last verified: 2020-01