NCT03546101

Brief Summary

Epstein-Barr virus (EBV) is one of several herpesviruses that cause disease in humans. EBV virus has an oncogenic potential, and it has been associated with the development of a wide range of cancers. Previous studies have shown a close association between EBV and Post-Transplant Lymphoproliferative disorder (PTLD) in transplant recipients. As part of a preventive approach against PTLD, several transplantation units now monitor the occurrence of EBV-DNAemia after transplantation. However, there is little evidence to guide this strategy; nor is there consensus concerning either the best specimen to use for EBV analysis (whole blood or plasma). In this study investigators aim to optimise and validate a polymerase chain reaction (PCR)-test for EBV-DNA on, respectively, whole blood, plasma and a combination of plasma and lymphocytes. The investigators wish to determine which of the three tests best predicts current and future risk of development of EBV-related diseases such as mononucleosis and PTLD.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,527

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Nov 2017

Longer than P75 for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2017

Completed
6 months until next milestone

First Submitted

Initial submission to the registry

May 7, 2018

Completed
1 month until next milestone

First Posted

Study publicly available on registry

June 6, 2018

Completed
9 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2019

Completed
4.2 years until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2023

Completed
Last Updated

October 31, 2023

Status Verified

December 1, 2022

Enrollment Period

1.3 years

First QC Date

May 7, 2018

Last Update Submit

October 27, 2023

Conditions

Outcome Measures

Primary Outcomes (2)

  • Incidence of proven EBV disease

    Symptoms compatible with EBV disease combined with a tissue biopsy positive for EBV

    From time of blood sampling up to 24 months.

  • Incidence of probable EBV disease

    Symptoms compatible with EBV disease combined with a positive EBV-PCR

    From time of blood sampling up to 24 months.

Study Arms (3)

Department of Kidney Medicine

Primarily transplant recipients undergoing monitoring for EBV and patients suspected for having PTLD.

Department of Hematology

Patients diagnosed with PTLD and other kinds of lymphoma. Patients undergoing hematopoietic stem cell transplantation and patients with hemophagocytosis.

Department of pediatrics

Children undergoing transplantation. Children diagnosed with hemophagocytic lymphohistiocytosis.

Eligibility Criteria

Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Transplant recipients undergoing monitoring for EBV and patients suspected or diagnosed with PTLD. Patients undergoing hematopoietic stem cell transplantation and patients with hemophagocytosis. Children undergoing transplantation. Children diagnosed with hemophagocytic lymphohistiocytosis.

You may qualify if:

  • Patients suspected for having EBV disease.

You may not qualify if:

  • If the patients has any contraindications for blood sampling.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Aarhus University Hospital

Aarhus, Central Region of Denmark, 8200, Denmark

Location

Related Publications (1)

  • Ludvigsen LUP, Andersen AS, Hamilton-Dutoit S, Jensen-Fangel S, Bottger P, Handberg KJ, Ivarsen P, d'Amore F, Bibby BM, Albertsen BK, Jespersen B, Thomsen MK. A prospective evaluation of the diagnostic potential of EBV-DNA in plasma and whole blood. J Clin Virol. 2023 Oct;167:105579. doi: 10.1016/j.jcv.2023.105579. Epub 2023 Aug 30.

Biospecimen

Retention: SAMPLES WITH DNA

Blood samples

MeSH Terms

Conditions

Infectious MononucleosisEpstein-Barr Virus InfectionsLymphohistiocytosis, Hemophagocytic

Condition Hierarchy (Ancestors)

Herpesviridae InfectionsDNA Virus InfectionsVirus DiseasesInfectionsLeukocyte DisordersHematologic DiseasesHemic and Lymphatic DiseasesLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesTumor Virus InfectionsHistiocytosis, Non-Langerhans-CellHistiocytosis

Study Officials

  • Lene Ugilt, MD

    University of Aarhus

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 7, 2018

First Posted

June 6, 2018

Study Start

November 1, 2017

Primary Completion

March 1, 2019

Study Completion

May 1, 2023

Last Updated

October 31, 2023

Record last verified: 2022-12

Data Sharing

IPD Sharing
Will not share

Locations