Antioxidant Responses to Exercise and Cherry Consumption

NCT03522779 | Completed

• 1 other identifier: STUDY00003902
• Study Type: interventional
• Target: 13
• Locations: 1 country
• Sites: 1

Brief Summary

To date, there are no published studies on the effects of tart cherry consumption on postprandial antioxidant and oxidative stress levels following a high-fat meal. Further, no one has looked at the effects of prior exercise on a high-fat meal that includes high phytonutrients like tart cherries. Therefore, the purpose of this study is to examine the effects of tart cherry consumption on antioxidant and oxidative stress levels in human plasma after consumption of a high-fat meal, as well as the postprandial impact of prior exercise in combination with tart cherry consumption on a high-fat meal.

Conditions

Dietary Modification

Outcome Measures

Primary Outcomes (2)

• Change in Antioxidant levels

  Plasma will be tested for total anthocyanins content by HPLC-UV/VIS as well as HPLC-ESI-MS. Antioxidant capacity measurements will be performed by FRAP assays.

  Change from baseline to 3 hours postprandially

• Change in oxidative stress levels

  Oxidative stress measurements will be performed by H-ORACFL assays.

  Change from baseline to 3 hours postprandially

Study Arms (4)

High-fat meal + placebo

PLACEBO COMPARATOR

Participants will complete a high-fat meal challenge with a placebo beverage.The placebo will be designed to match the tart cherry juice for volume and macronutrient content, but without the phytonutrient content of the tart cherries.The high-fat breakfast meal will consist of 2 Sausage Biscuits (McDonalds Corporation). Blood draws will then be obtained at 1, 2 and 3 hours postprandially for antioxidant and oxidative stress measurements.

Other: High-fat meal

High-fat meal + tart cherry

EXPERIMENTAL

Participants will complete a high-fat meal challenge with a tart cherry juice concentrate. 60 mL of montmorency tart cherry concentrate will be diluted with 100 mL of water.The high-fat breakfast meal will consist of 2 Sausage Biscuits (McDonalds Corporation). Blood draws will then be obtained at 1, 2 and 3 hours postprandially for antioxidant and oxidative stress measurements.

Dietary Supplement: Tart cherry Juice; Other: High-fat meal

Exercise + high-fat meal + placebo

PLACEBO COMPARATOR

Participants will perform an acute bout of submaximal aerobic exercise 15 hours prior to the high-fat meal test. The exercise will consist of 30 minutes of treadmill running at 70% of each participants' calculated heart rate reserve. The following morning participants will complete a high-fat meal challenge with a placebo beverage.The placebo will be designed to match the tart cherry juice for volume and macronutrient content, but without the phytonutrient content of the tart cherries.The high-fat breakfast meal will consist of 2 Sausage Biscuits (McDonalds Corporation). Blood draws will then be obtained at 1, 2 and 3 hours postprandially for antioxidant and oxidative stress measurements.

Other: High-fat meal; Other: Exercise

Exercise + high-fat meal + tart cherry

EXPERIMENTAL

Participants will perform an acute bout of submaximal aerobic exercise 15 hours prior to the high-fat meal test. The exercise will consist of 30 minutes of treadmill running at 70% of each participants' calculated heart rate reserve.The following morning participants will complete a high-fat meal challenge with a tart cherry juice concentrate. 60 mL of montmorency tart cherry concentrate will be diluted with 100 mL of water.The high-fat breakfast meal will consist of 2 Sausage Biscuits (McDonalds Corporation). Blood draws will then be obtained at 1, 2 and 3 hours postprandially for antioxidant and oxidative stress measurements.

Dietary Supplement: Tart cherry Juice; Other: High-fat meal; Other: Exercise

Interventions

Tart cherry Juice DIETARY_SUPPLEMENT

Tart cherry juice will be used to compare antioxidant and oxidative stress levels in human plasma following a high fat meal.

Exercise + high-fat meal + tart cherry; High-fat meal + tart cherry

High-fat meal OTHER

A high fat meal will be given to elicit postprandial oxidative stress.

Exercise + high-fat meal + placebo; Exercise + high-fat meal + tart cherry; High-fat meal + placebo; High-fat meal + tart cherry

Exercise OTHER

The exercise will consist of 30 minutes of treadmill running at 70% of each participants' calculated heart rate reserve.

Exercise + high-fat meal + placebo; Exercise + high-fat meal + tart cherry

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Males, 18-30 years old, with normal body weight based on body mass index (BMI=18-24.9kg/m2) or are of normal weight based on body composition analysis using the DXA (14-24% body fat). Must also participate in 3 or more hours per week of exercise with 1.5 or more of those hours being aerobic exercise.

You may not qualify if:

• Anyone with a chronic disease, medication use, digestive disorders, any supplement use, and anyone who will not consume tart cherry product. Anyone who is on a weight loss program, or planning to alter their current exercise program before the study would finish. Anyone who has lost or gained more than 5% of their body weight in the past 3 months. Anyone who has allergies to food, is vegan or vegetarian, or is on a medically prescribed diet.

Sponsors & Collaborators

• University of Georgia: lead

Study Sites (1)

University of Georgia - Department of Foods and Nutrition

Athens, Georgia, 30602, United States

Location

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Jamie Cooper, PhD

  University of Georgia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Jamie Cooper

Study Record Dates

• First Submitted: January 19, 2017
• First Posted: May 11, 2018
• Study Start: November 1, 2016
• Primary Completion: July 1, 2017
• Study Completion: July 1, 2017
• Last Updated: May 11, 2018

Record last verified: 2018-04

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)