Effect of Smoking on the Expression of Gelatinases in Chronic Periodontitis

NCT03426241 | Completed

• 1 other identifier: BaskentU2
• Study Type: observational
• Target: 80
• Locations: 0 countries
• Sites: N/A

Brief Summary

This study comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the hypothesis of this study was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Conditions

Periodontitis; Smoking

Keywords

chronic periodontitis; smoking; mmp-2; mmp-9

Outcome Measures

Primary Outcomes (1)

• expression of gelatinases levels in gingival tissue among groups

  In chronic periodontitis group, after recording the clinical parameters, gingival tissues were harvested from the deepest periodontal pocket. Whereas in periodontally healthy group, gingival tissues were collected during crown-lengthening surgery or during an extraction of a teeth for orthodontic reasons. In both groups the samples include both sulcus/pocket epithelium and underlying connective tissues. The expression of MMP-2 and MMP-9 is measured immunohistochemically

  day 1

Secondary Outcomes (2)

• difference in probing depths among groups

  day o

• difference in probing depths among groups

  day 0

Study Arms (4)

smoking chronic periodontitis

Other: no intervention

non-smoking periodontitis

Other: no intervention

smoking healthy

Other: no intervention

non-smoking healthy

Other: no intervention

Interventions

no intervention OTHER

this is cross-sectional study

non-smoking healthy; non-smoking periodontitis; smoking chronic periodontitis; smoking healthy

Eligibility Criteria

• Age: 16 Years - 62 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)
• Sampling Method: Probability Sample

Study Population

A total of 80 subjects (35 females, 45 males, with an age range of 16-62 years) were included in this study from those patients seeking dental treatment at the Baskent University School of Dentistry

You may qualify if:

• A total of 80 subjects (35 females, 45 males, with an age range of 16-62 years) were included in this study from those patients seeking dental treatment at the Baskent University School of Dentistry between February 2010 and December 2012.

You may not qualify if:

• Patients with systemic disease;
• pregnant and/or lactating women;
• patients taking any medications that may influence the study.

Sponsors & Collaborators

• Baskent University: lead

MeSH Terms

Conditions

Periodontitis; Smoking; Chronic Periodontitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Behavior; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Clinician in periodontology department

Study Record Dates

• First Submitted: January 29, 2018
• First Posted: February 8, 2018
• Study Start: February 10, 2010
• Primary Completion: December 29, 2012
• Study Completion: December 30, 2012
• Last Updated: February 8, 2018

Record last verified: 2018-02

Data Sharing

• IPD Sharing: Will not share