Cell Signaling and Resistance to Oxidative Stress: Effects of Aging and Exercise

NCT03419988 | Completed

• 1 other identifier: R15AG055077
• Study Type: interventional
• Target: 46
• Locations: 1 country
• Sites: 1

Brief Summary

Advanced age is the main risk factor for chronic diseases such as cardiovascular disease, type 2 diabetes, Alzheimer's disease and cancer. One reason may be due to decreased resistance to oxidative stress as antioxidant defenses and cell protection is reduced with aging. This has been shown in animal studies and also that the impairment can be somewhat restored with exercise. This will be the first study to test this in humans by comparing young and older inactive adults before and after an exercise intervention, a practical and cost-effective intervention that can have tremendous public health impact by lowering risk for disease and medical-related costs.

Conditions

Aging

Keywords

Nrf2

Outcome Measures

Primary Outcomes (2)

• Cell signaling

  Nuclear Nrf2 protein content in peripheral mononuclear cells (PBMCs) in response to acute exercise

  8 weeks

• Resistance to oxidative stress

  F2-isoprostane response to forearm ischemia-reperfusion

  8 weeks

Secondary Outcomes (1)

• Cell signaling

  8 weeks

Study Arms (2)

Exercise Intervention

EXPERIMENTAL

8-weeks exercise intervention: 3-days per week for 45-55 minutes per session

Behavioral: Exercise Intervention

Control

NO INTERVENTION

8-weeks control: asked not to change anything or start exercising.

Interventions

Exercise Intervention BEHAVIORAL

24 sessions of aerobic exercise

Exercise Intervention

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Men and women, 18-28 years or 60 years and older
• Competent to independently give informed consent
• Successful completion of screening
• No regular exercise for the past 6-months (by self-report)
• A score below 3.0 on the Historical Lifetime Physical Activity Questionnaire

You may not qualify if:

• Estrogen supplementation within the previous 6 months
• Use of anti-oxidant supplements, in excess of standard multi-vitamins (1 tablet/day)
• Current smoker
• Body Mass Index (BMI) ≤33 kg/m2 (Class I Obesity)
• Any chronic illness that could affect outcome measures, including diabetes, liver or renal disease, or cancer (other than skin cancer)
• History of a myocardial infarction within the last 6 months, clinically significant aortic stenosis, use of cardiac defibrillator, or uncontrolled angina
• Clinically significant arrhythmia on a resting EKG or significant EKG changes during the baseline maximal oxygen consumption (VO2 max) test
• Any other condition that would contraindicate maximal exercise testing, including elevated blood pressure at rest (systolic BP \>150 or diastolic BP \>90 mm Hg on at least 2 measurements, at least 10 minutes apart) or musculoskeletal problems

Sponsors & Collaborators

• Northern Arizona University: lead

Study Sites (1)

Northern Arizona University

Flagstaff, Arizona, 86011, United States

Location

Related Publications (1)

• Ostrom EL, Traustadottir T. Aerobic exercise training partially reverses the impairment of Nrf2 activation in older humans. Free Radic Biol Med. 2020 Nov 20;160:418-432. doi: 10.1016/j.freeradbiomed.2020.08.016. Epub 2020 Aug 28.

  PMID: 32866619 DERIVED

Study Officials

• Tinna Traustadóttir, PhD

  Northern Arizona University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor, Biological Sciences

Model Details: Randomized control trial: exercise intervention versus non-exercise control

Study Record Dates

• First Submitted: January 11, 2018
• First Posted: February 5, 2018
• Study Start: June 1, 2017
• Primary Completion: May 30, 2020
• Study Completion: June 30, 2020
• Last Updated: August 26, 2020

Record last verified: 2020-08

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)