NCT03325270

Brief Summary

Rationale: According to the WHO, iron deficiency is still the most common and widespread nutritional disorder in the world. Current iron supplements have limitations in terms of bioavailability and tolerability. Prebiotic fibers, such as galacto-oligosaccharides (GOS), selectively enhance growth of beneficial colonic bacteria. Prebiotics in general enhance the production of short-chain fatty acids (SCFAs) and thereby decrease luminal pH. Through the reduction in colonic pH, prebiotics can enhance absorption of minerals such as calcium and magnesium and they have been proposed to potentially improve iron absorption as well. The primary objective of this study is to investigate the effect of an addition of GOS to an iron supplement on iron absorption in iron deplete, healthy, normal weight women.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
46

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jan 2018

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

October 25, 2017

Completed
5 days until next milestone

First Posted

Study publicly available on registry

October 30, 2017

Completed
2 months until next milestone

Study Start

First participant enrolled

January 10, 2018

Completed
4 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 28, 2018

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 28, 2018

Completed
Last Updated

January 16, 2019

Status Verified

January 1, 2019

Enrollment Period

4 months

First QC Date

October 25, 2017

Last Update Submit

January 15, 2019

Conditions

Keywords

prebioticsGalacto-oligosaccharidesIron-deficiency

Outcome Measures

Primary Outcomes (1)

  • fractional iron absorption

    Fractional iron absorption will be calculated based on the shift of the iron isotope ratios in the collected blood samples after the administration of several isotopically labelled iron supplements (days 0, 2 16, 18, 20, 37, 39, 41).Fractional iron absorption will be measured as erythrocyte incorporation of the naturally occurring iron forms with different masses used to label the iron supplements.

    2 months

Study Arms (3)

ferrous fumarate

EXPERIMENTAL

labeled iron as Ferrous Fumarate

Dietary Supplement: labeled ferrous fumarate

ferrous fumarate and GOS

EXPERIMENTAL

labeled ferrous fumarate + prebiotics

Dietary Supplement: labeled ferrous fumarate + prebiotics

ferrous sulfate and GOS

EXPERIMENTAL

labeled ferrous sulfate + prebiotics

Dietary Supplement: labeled ferrous sulfate + prebiotics

Interventions

labeled ferrous fumarateDIETARY_SUPPLEMENT

labeled iron as Ferrous Fumarate

ferrous fumarate

labeled iron as Ferrous Fumarate + galacto-oligosaccharides

ferrous fumarate and GOS

labeled iron as Ferrous Sulfate + galacto-oligosaccharides

ferrous sulfate and GOS

Eligibility Criteria

Age18 Years - 45 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsOnly female participants are being studied
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Female, 18 to 45 years old
  • SF levels \<20 µg/L
  • Normal body Mass Index (18.5-24.9 kg/m2)
  • Body weight \<70 kg
  • Signed informed consent

You may not qualify if:

  • Severe anaemia (Hb \< 80 g/L)
  • Elevated CRP \>10.0 mg/L
  • Any metabolic, gastrointestinal kidney or chronic disease such as diabetes, renal failure, hepatic dysfunction, hepatitis, hypertension, cancer or cardiovascular diseases (according to the participants own statement)
  • Continuous/long-term use of medication during the whole studies (except for contraceptives)
  • Consumption of mineral and vitamin supplements within 2 weeks prior to 1st supplement administration, including pre- and-or probiotics supplements (excluding foods and beverages with life cultures such as yoghurt, raw milk cheese and kombucha)
  • Blood transfusion, blood donation or significant blood loss (accident, surgery) over the past 4 months
  • Difficulties with blood sampling
  • Use of antibiotics over the past month
  • Known hypersensitivity to iron supplements in the given amount, GOS, or lactose
  • Women who are pregnant or breast feeding
  • Women who intend become pregnant during the course of the study
  • Lack of safe contraception, defined as: Female participants of childbearing potential, not using and not willing to continue using a medically reliable method of contraception for the entire study duration, such as oral, injectable, or implantable contraceptives, or intrauterine contraceptive devices, or who are not using any other method considered sufficiently reliable by the investigator in individual cases
  • Known or suspected non-compliance, drug or alcohol (more than 2 drinks/day) abuse -
  • Smokers (\> 1 cigarette per week)
  • Inability to follow the procedures of the study, e.g. due to language problems, self-reported psychological disorders, etc. of the participant
  • +1 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

ETH Zurich

Zurich, 8092, Switzerland

Location

Related Publications (1)

  • Jeroense FMD, Michel L, Zeder C, Herter-Aeberli I, Zimmermann MB. Consumption of Galacto-Oligosaccharides Increases Iron Absorption from Ferrous Fumarate: A Stable Iron Isotope Study in Iron-Depleted Young Women. J Nutr. 2019 May 1;149(5):738-746. doi: 10.1093/jn/nxy327.

MeSH Terms

Conditions

Anemia, Iron-Deficiency

Interventions

Prebiotics

Condition Hierarchy (Ancestors)

Anemia, HypochromicAnemiaHematologic DiseasesHemic and Lymphatic DiseasesIron DeficienciesIron Metabolism DisordersMetabolic DiseasesNutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Dietary FiberDietary CarbohydratesCarbohydratesPolysaccharides, BacterialPolysaccharidesFoodDiet, Food, and NutritionPhysiological PhenomenaDietary SupplementsFood and Beverages

Study Officials

  • Michael Zimmermann, Prof. Dr.

    University of Zurich

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Head of Laboratory of Human Nutrition

Study Record Dates

First Submitted

October 25, 2017

First Posted

October 30, 2017

Study Start

January 10, 2018

Primary Completion

April 28, 2018

Study Completion

April 28, 2018

Last Updated

January 16, 2019

Record last verified: 2019-01

Data Sharing

IPD Sharing
Will not share

Locations