NCT03301753

Brief Summary

Today the majority of pregnant women in the United States are either overweight or obese at conception with their offspring having greater adiposity at birth, a 2-fold greater risk of later obesity, and neonatal insulin resistance. It was long thought that breast milk composition was fairly uniform among women, having been optimized through evolutionary time to provide adequate sole nutrition for the growing infant regardless of the environmental circumstances. However, recent evidence shows that breast milk is a highly complex fluid with significant inter-individual variation in hormonal and cytokine concentrations, human milk oligosaccharides (HMOs) and other features. Pervasive maternal obesity and gestational diabetes are evolutionarily novel conditions for the human species but little effort has yet been made to systematically examine how they are associated with breast milk adipose-tissue derived hormone and cytokine (adipocytokine) variation, HMOs, or other features, or whether that variation relates to infant metabolic status. The objective of this study is to comprehensively assess the "lactational programming" hypothesis, that is, whether or not recently documented variation in breast-milk composition is related to both maternal and infant metabolic status. The central hypothesis is that a graded, dose-response relationship between maternal adiposity and GDM exists with adipocytokine concentrations, HMOs, and other features in breast milk and that the milk concentrations of these features are associated with altered body composition in their exclusively breast-fed offspring. The results of the study will be used to design interventions to reduce maternal weight during pregnancy and lactation and to augment lactation education materials to focus on the needs of breastfeeding women with obesity and GDM.

Trial Health

75
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
2,050

participants targeted

Target at P75+ for all trials

Timeline
8mo left

Started Jul 2014

Longer than P75 for all trials

Geographic Reach
1 country

2 active sites

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress95%
Jul 2014Dec 2026

Study Start

First participant enrolled

July 1, 2014

Completed
3.2 years until next milestone

First Submitted

Initial submission to the registry

September 13, 2017

Completed
21 days until next milestone

First Posted

Study publicly available on registry

October 4, 2017

Completed
9.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2026

Last Updated

August 24, 2025

Status Verified

August 1, 2025

Enrollment Period

12.5 years

First QC Date

September 13, 2017

Last Update Submit

August 18, 2025

Conditions

Outcome Measures

Primary Outcomes (1)

  • Milk Composition Changes

    Breastmilk hormone concentration changes

    1-3 months post-partum

Secondary Outcomes (1)

  • Infant Body Composition Changes

    0-6 months of age

Eligibility Criteria

Age0 Years - 45 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64)
Sampling MethodNon-Probability Sample
Study Population

(Minneapolis site): In the Twin Cities metro area, subjects will be recruited from patients who attend medical group clinics for Ob/Gyn care in their second trimester of pregnancy through the various means, including through the OB/GYN department of the University of Minneosta Medical School via letter and follow-up phone call for final screening. (Oklahoma City site): Recruitment in the OKC metro area will be through OUHSC Ob/Gyn clinic, and local private practice OB/GYN clinics during the second trimester, through the La Leche League, and public announcements.

You may qualify if:

  • Pregnant females
  • to 45 years of age at the time of delivery
  • Pre-gravid BMI between 18.5 to 40 kg/m2
  • Healthy pregnancy defined as \<3 days in hospital following delivery
  • Report during enrollment procedures that they have social support for and intention to exclusively breastfeed for at least 3-months
  • If parity \>1, report that they successfully breast fed a previous pregnancy for at least 3 months
  • Singleton gestation
  • Term pregnancy (gestational age \>37 but \<42 weeks)
  • Infant birth weight \>2,500 grams but \<4,500 grams.

You may not qualify if:

  • Alcohol consumption \>1 drink per week during pregnancy/lactation
  • Tobacco consumption during pregnancy/lactation
  • History/current Type I or II diabetes
  • Inability to speak/understand English
  • Known congenital metabolic, endocrine disease, or congenital illness affecting infant feeding/growth

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

University of Minnesota School of Public Health

Minneapolis, Minnesota, 55454, United States

Location

University of Oklahoma Health Sciences Center

Oklahoma City, Oklahoma, 73104, United States

Location

Related Publications (1)

  • Sadr Dadres G, Whitaker KM, Haapala JL, Foster L, Smith KD, Teague AM, Jacobs DR Jr, Kharbanda EO, McGovern PM, Schoenfuss TC, Le LJ, Harnack L, Fields DA, Demerath EW. Relationship of Maternal Weight Status Before, During, and After Pregnancy with Breast Milk Hormone Concentrations. Obesity (Silver Spring). 2019 Apr;27(4):621-628. doi: 10.1002/oby.22409.

Related Links

Biospecimen

Retention: SAMPLES WITHOUT DNA

2 ml aliquots of breastmilk are stored for future chemical analysis.

MeSH Terms

Conditions

ObesityBreast Milk ExpressionInfant Nutrition DisordersDiabetes, Gestational

Condition Hierarchy (Ancestors)

OverweightOvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsBreast FeedingFeeding BehaviorBehaviorPregnancy ComplicationsFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesDiabetes MellitusGlucose Metabolism DisordersMetabolic DiseasesEndocrine System Diseases

Study Officials

  • David Fields, PhD

    University of Oklahoma

    PRINCIPAL INVESTIGATOR
  • Ellen Demerath, PhD

    University of Minnesota

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 13, 2017

First Posted

October 4, 2017

Study Start

July 1, 2014

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

December 31, 2026

Last Updated

August 24, 2025

Record last verified: 2025-08

Data Sharing

IPD Sharing
Will share

As required by the NICHD, we will upload our clinical subject data and non-genomic milk specimen analyses to the NICHD Data and Specimen Hub (DASH). Data submitted to NICHD DASH will be individual-level research data that has been de-identified and is usable for secondary analysis. This will include maternal and infant demographics, diet intake data, clinical status information, body weight and growth data, body composition data, infant feeding and breastfeeding data, maternal physical activity and behavioral data, milk compositional data, and infant fecal microbiome data. All research data submitted to NICHD DASH will be accompanied by proper documentation including study protocol, data collection instruments, data dictionaries, and data de-identification methodology.

Shared Documents
STUDY PROTOCOL, ICF
Time Frame
Within 6 months of funding end date
Access Criteria
Anyone who has access to the NIH/NICHD Data and Specimen Sharing Hub (DASH) will be able to access the IPD

Locations