Carbohydrate Availability and microRNA Expression

NCT03250234 | Completed Results

• 1 other identifier: 17-17H
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

This investigation will examine the impact of skeletal muscle glycogen stores on skeletal muscle and circulating microRNA expression and exogenous carbohydrate oxidation. Primary Objective Determine the influence of carbohydrate availability (e.g., glycogen depletion and repletion) on skeletal muscle microRNA expression, and if changes in circulating microRNA are reflective of changes in skeletal muscle microRNA. Secondary Objective Determine how initiation of exercise with adequate or low glycogen stores effects exogenous carbohydrate efficiency.

Conditions

Glycogen Depletion

Keywords

microRNA, carbohydrate oxidation

Outcome Measures

Primary Outcomes (1)

• Fold Change in microRNA Expression From Baseline

  Determine the influence of low or adequate glycogen on expression of skeletal muscle and circulating microRNA. Expression of microRNA was determined using real time polymerase chain reaction analysis. microRNA expressions were calculated as a fold change relative to baseline.

  24 hours

Secondary Outcomes (1)

• Exogenous Carbohydrate Oxidation

  80 minutes

Study Arms (2)

Adequate Carbohydrate

OTHER

Carbohydrate beverage (1 g/kg/hr) Adequate carbohydrate diet 6.0 g/kg/d

Other: Adequate Carbohydrate

Low Carbohydrate

OTHER

Non-nutritive control beverage. Low carbohydrate diet 1.2 g/kg/d

Other: Low Carbohydrate

Interventions

Adequate Carbohydrate OTHER

During the 3-hr recovery period after glycogen depletion participants will consume a carbohydrate (1 g/kg/hr) beverage For the day participants will consume a diet of 6.0 g/kg/d carbohydrate following glycogen depletion

Adequate Carbohydrate

Low Carbohydrate OTHER

During the 3-hr recovery period after glycogen depletion participants will consume a nutrient free beverage. For the day participants will consume a diet of 1.2 g/kg/d carbohydrate following glycogen depletion

Low Carbohydrate

Eligibility Criteria

• Age: 18 Years - 39 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Men and women aged 18 - 39 years
• Weight stable (±5 lbs) for at least 2 months prior to the start of the study
• Body mass index (BMI) between 18.5-30 kg/m2
• Recreationally active based on assessment of physical activity history 2-4 days per week aerobic and/or resistance exercise
• Refrain from taking any NSAIDS (i.e., aspirin, Advil®, Aleve®, Naprosyn®, or any aspirin-containing product for 10 days before and at least 5 days AFTER each muscle biopsy. (\*Tylenol® or acetaminophen is ok to use if needed for discomfort)
• Refrain from the use of alcohol and nicotine for the duration of the study
• Females must be on contraception (e.g., oral birth control, NuvaRing®, Depo Provera®, etc.)
• Supervisor approved leave status for federal civilian employees working within the US Army Natick Soldier Systems Center

You may not qualify if:

• Metabolic or cardiovascular abnormalities, gastrointestinal disorders (i.e., kidney disease, diabetes, cardiovascular disease, etc.)
• Disease or medication (i.e., diabetes medications, statins, corticosteroids, etc) that affects macronutrient utilization and/or the ability to participate in strenuous exercise
• Allergies or intolerance to foods (including but not limited to lactose intolerance/milk allergy), vegetarian practices, or medications (including, but not limited to, lidocaine or phenylalanine) to be utilized in the study
• Anemia (HCT \< 38) and Sickle Cell Anemia/Trait
• Abnormal prothrombin time (PT)/ partial thromboplastin time (PTT) test or problems with blood clotting
• Present condition of alcoholism, use of nutritional/sports supplements, anabolic steroids, or other substance abuse issues
• Musculoskeletal injuries that compromise the ability to exercise
• Blood donation within 8 weeks of beginning the study
• Pregnancy

Sponsors & Collaborators

• United States Army Research Institute of Environmental Medicine: lead

Study Sites (1)

USARIEM

Natick, Massachusetts, 01760, United States

Location

Results Point of Contact

• Title: Dr. Lee Margolis
• Organization: USARIEM

lee.m.margolis.civ@mail.mil

508-206-2335

Publication Agreements

• PI is Sponsor Employee: Yes

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: FED
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: July 19, 2017
• First Posted: August 15, 2017
• Study Start: August 1, 2017
• Primary Completion: May 3, 2018
• Study Completion: May 3, 2018
• Last Updated: September 2, 2021
• Results First Posted: September 2, 2021

Record last verified: 2021-08

Locations

US (1)