Vascular Health With Immobilization and Exercise Training

NCT03054376 | Completed

• 1 other identifier: ANG-CV- IMMOB
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

The study includes healthy young habitually active subjects. All subjects are subjected to two weeks of immobilization of one leg followed by four weeks of training of both legs. The subjects are randomized to either one-leg training of the non-immobilized leg during the two-week immobilization period or to no training of the non-immobilized leg during the immobilization period. Measures include skeletal muscle morphology, markers of angiogenesis and apoptosis, muscle metabolic markers, vascular function, vascular proteins and performance.

Conditions

Immobilization, Tonic; Physical Activity

Keywords

Immobilization; Angiogenesis; Skeletal muscle; Exercise training; Vascular function

Outcome Measures

Primary Outcomes (4)

• Angiogenesis

  Skeletal muscle capillarization-comparison between legs

  Change from baseline to after 2 weeks of immobilization

• Vascular function

  Measurements of skeletal muscle blood flow by ultrasound doppler in response to infusion of vasodilators/constrictors-comparison between legs

  Change from baseline to after 2 weeks of immobilization

• Angiogenesis

  Skeletal muscle capillarization-comparison between legs

  Change from after immobilization to after 4 weeks of training

• Vascular function

  Measurements of skeletal muscle blood flow by ultrasound doppler in response to infusion of vasodilators/constrictors-comparison between legs

  Change from after immobilization to after 4 weeks of training

Secondary Outcomes (9)

• Vascular and metabolic proteins

  Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training

• Arterial blood pressure

  Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training

• Knee extensor performance

  Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training

• Maximal oxygen uptake

  Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training

• Body composition

  Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training

Study Arms (2)

Immobilization

ACTIVE COMPARATOR

After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will not be trained. After the two weeks the subjects will train both legs for four weeks.

Other: Immobilization; Other: Exercise training with both legs

Training of non-immobilized leg

ACTIVE COMPARATOR

After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will be trained. After the two weeks the subjects will train both legs for four weeks.

Other: Immobilization; Other: Training with non immobilized leg; Other: Exercise training with both legs

Interventions

Immobilization OTHER

Full length cast of one leg R/L randomized

Immobilization; Training of non-immobilized leg

Training with non immobilized leg OTHER

Exercise training will be conducted with the non-immobilized leg during the immobilization period of two weeks

Training of non-immobilized leg

Exercise training with both legs OTHER

Exercise training will be conducted regularly on a cycle ergometer during a four week period

Immobilization; Training of non-immobilized leg

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy
• BMI \<25
• Habitually/moderately physically active

You may not qualify if:

• Known chronic medical disorder
• Smoking
• Excessive alcohol intake
• Known genetic/familial predisposition for thrombosis
• Hormonal birth control
• High level strength or endurance training on a regular basis

Sponsors & Collaborators

• University of Copenhagen: lead
• Johannes Gutenberg University Mainz: collaborator
• University of Leeds: collaborator
• Liverpool John Moores University: collaborator
• Radboud University Medical Center: collaborator

Study Sites (1)

University of Copenhagen

Copenhagen, Denmark

Location

Related Publications (1)

• Gliemann L, Rytter N, Jorgensen TS, Piil P, Carter H, Nyberg M, Grassi M, Daumer M, Hellsten Y. The Impact of Lower Limb Immobilization and Rehabilitation on Angiogenic Proteins and Capillarization in Skeletal Muscle. Med Sci Sports Exerc. 2021 Sep 1;53(9):1797-1806. doi: 10.1249/MSS.0000000000002665.

  PMID: 33787530 DERIVED

MeSH Terms

Conditions

Immobility Response, Tonic; Motor Activity

Interventions

Immobilization; Exercise

Condition Hierarchy (Ancestors)

Behavior, Animal; Behavior

Intervention Hierarchy (Ancestors)

Investigative Techniques; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: Parallel randomized to training or no training of non-immobilized leg during immobilization of the other leg

Study Record Dates

• First Submitted: February 5, 2017
• First Posted: February 15, 2017
• Study Start: March 15, 2017
• Primary Completion: October 20, 2022
• Study Completion: October 20, 2022
• Last Updated: November 2, 2022

Record last verified: 2022-11

Data Sharing

• IPD Sharing: Will not share

Locations

DK (1)