NCT02993484

Brief Summary

The overall aim of this study is to examine the role of mitochondrial respiration in human diabetic tissue before and after ischemia. Furthermore we will examine the ability of ischemic preconditioning (IPC) to preserve the mitochondrial function and hemodynamic performance of both non-diabetic and diabetic fibers after ischemia. To increase our understanding on the metabolic changes during ischemia in both non-diabetic and diabetic tissue we will use Dimethyl Malonate and examine the impact of this blockade on post-ischemic mitochondrial respiration.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
39

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Dec 2016

Typical duration for not_applicable

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

December 1, 2016

Completed
7 days until next milestone

First Submitted

Initial submission to the registry

December 8, 2016

Completed
7 days until next milestone

First Posted

Study publicly available on registry

December 15, 2016

Completed
2.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2019

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

February 1, 2019

Completed
Last Updated

June 7, 2019

Status Verified

October 1, 2018

Enrollment Period

2.2 years

First QC Date

December 8, 2016

Last Update Submit

June 6, 2019

Conditions

Outcome Measures

Primary Outcomes (1)

  • Mitochondrial respiration of individual complexes

    Mitochondrial respiration of individual complexes will serve as primary outcome and will be examined by oxygraph technique.

    Outcome will be assessed within a year when the inclusion of patients is complete

Secondary Outcomes (1)

  • Hemodynamic performance

    Outcome will be assessed within a year when the inclusion of patients is complete

Study Arms (8)

non-diabetic Sham control

EXPERIMENTAL

Heart tissue obtained from non-diabetic patients undergoing surgery will not receive drugs or ischemia

Other: Sham

non-diabetic Ischemia reperfusion

EXPERIMENTAL

Heart tissue from non-diabetic patients undergoing surgery will not receive drugs but will receive ischemia

Other: Ischemia reperfusion

non-diabetic Ischemic preconditioning

EXPERIMENTAL

Heart tissue from non-diabetic patients undergoing surgery will not receive drugs but will receive short periods of ischemia prior to index ischemia

Biological: Ischemic preconditioning

non-diabetic Dimethyl malonate

EXPERIMENTAL

Heart tissue from non-diabetic patients undergoing surgery will receive a drug (Dimethyl Malonate) prior to index ischemia

Drug: Dimethyl Malonate

Diabetic Sham control

EXPERIMENTAL

Heart tissue obtained from diabetic patients undergoing surgery will not receive drugs or ischemia

Other: Sham

Diabetic Ischemia reperfusion

EXPERIMENTAL

Heart tissue from diabetic patients undergoing surgery will not receive drugs but will receive ischemia

Other: Ischemia reperfusion

Diabetic Ischemic preconditioning

EXPERIMENTAL

Heart tissue from diabetic patients undergoing surgery will not receive drugs but will receive short periods of ischemia prior to index ischemia

Biological: Ischemic preconditioning

Diabetic Dimethyl malonate

EXPERIMENTAL

Heart tissue from diabetic patients undergoing surgery will receive a drug (Dimethyl Malonate) prior to index ischemia

Drug: Dimethyl Malonate

Interventions

Heart tissue will be obtained from patients undergoing heart surgery. The tissue will be used to test the properties of ischemia and different cardioprotective interventions.

Also known as: Dimethyl Malonate; PubChem Substance ID 24848281
Diabetic Dimethyl malonatenon-diabetic Dimethyl malonate

Heart tissue will be obtained from patients undergoing heart surgery. The tissue will be used to test the properties of ischemia and different cardioprotective interventions.

Diabetic Ischemic preconditioningnon-diabetic Ischemic preconditioning

Heart tissue will be obtained from patients undergoing heart surgery. The tissue will be used to test the properties of ischemia and different cardioprotective interventions.

Diabetic Ischemia reperfusionnon-diabetic Ischemia reperfusion
ShamOTHER

Heart tissue will be obtained from patients undergoing heart surgery. The tissue will be used to test the properties of ischemia and different cardioprotective interventions.

Diabetic Sham controlnon-diabetic Sham control

Eligibility Criteria

Age18 Years - 85 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Patients undergoing CABG or other surgery where extracorporal circulation will be used.

You may not qualify if:

  • raise in ischemic markers within 4 weeks
  • ejection fraction \<30
  • Atrial fibrilation
  • Oral opioid treatment

Contact the study team to confirm eligibility.

Sponsors & Collaborators

MeSH Terms

Conditions

Myocardial Reperfusion Injury

Interventions

methyl malonateIschemic Preconditioningsalicylhydroxamic acid

Condition Hierarchy (Ancestors)

CardiomyopathiesHeart DiseasesCardiovascular DiseasesMyocardial IschemiaVascular DiseasesReperfusion InjuryPostoperative ComplicationsPathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

TherapeuticsInvestigative Techniques

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 8, 2016

First Posted

December 15, 2016

Study Start

December 1, 2016

Primary Completion

February 1, 2019

Study Completion

February 1, 2019

Last Updated

June 7, 2019

Record last verified: 2018-10

Data Sharing

IPD Sharing
Will not share