The Effect of Dopamine on Pulmonary Diffusion and Capillary Blood Volume During Exercise

NCT02965963 | Completed

• 1 other identifier: Pro00067664
• Study Type: interventional
• Target: 49
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to examine the effect of dopamine infusion and dopamine-2 receptor blockade on pulmonary capillary blood volume, diffusion, and the hemodynamic variables of pulmonary artery pressure, cardiac output, and pulmonary vascular resistance during exercise. Secondarily, this study will examine the effect of dopamine infusion and dopamine-2 receptor blockade on exercise tolerance.

Conditions

Health

Outcome Measures

Primary Outcomes (5)

• Change in Pulmonary Capillary Blood Volume

  Roughton and Forster's Three FIO2 DLCO Method at Rest, 60% of Vo2max, and 85% of Vo2max

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

• Diffusing Capacity for Carbon Monoxide

  Roughton and Forster's Three FIO2 DLCO Method at Rest, 60% of Vo2max, and 85% of Vo2max

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

• Pulmonary Artery Systolic Pressure

  Non-invasive estimation using Doppler echocardiography at Rest \& 60% of Vo2max

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

• Cardiac Output

  Non-invasive estimation using trans-thoracic impedance cardiography at rest, 60% of Vo2max, and 85% of Vo2max

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

• Pulmonary Vascular Resistance

  Calculation

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

Secondary Outcomes (2)

• Exercise Tolerance

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

• Exertional Dyspnea

  Dopamine (Day 1), Metoclopramide (Day 2), Placebos (Day 3) *order randomized

Study Arms (3)

Dopamine

EXPERIMENTAL

Dependent variables measured with intravenous low dose dopamine infusion at rest, 60%, and 85% of VO2max

Drug: Dopamine; Other: Rest; Other: Exercise - 60%; Other: Exercise - 85%

Metoclopramide

EXPERIMENTAL

Dependent variables measured with oral metoclopramide ingestion at rest, 60%, and 85% of VO2max

Drug: Metoclopramide; Other: Rest; Other: Exercise - 60%; Other: Exercise - 85%

Placebos

EXPERIMENTAL

Dependent variables measured with orally ingested placebo pill and intravenous saline at rest, 60%, and 85% of VO2max

Drug: Placebos; Other: Rest; Other: Exercise - 60%; Other: Exercise - 85%

Interventions

Dopamine DRUG

Dopamine

Metoclopramide DRUG

Metoclopramide

Placebos DRUG

Placebos

Rest OTHER

Dopamine; Metoclopramide; Placebos

Exercise - 60% OTHER

60% of VO2max

Dopamine; Metoclopramide; Placebos

Exercise - 85% OTHER

85% of VO2max

Dopamine; Metoclopramide; Placebos

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Physically active (exercising \>2 times per week)
• BMI \< 30kg/m2
• No known cardiac or pulmonary disease

You may not qualify if:

• Known cardiac or pulmonary diseases / abnormalities
• Use of medications that could interfere with dopaminergic pathways (i.e. dopaminergic agonists / antagonists, alcohol, central nervous system depressants, and serotonergic drugs)
• BMI \> 30kg/m2
• Female subjects must not be pregnant

Sponsors & Collaborators

• University of Alberta: lead
• Natural Sciences and Engineering Research Council, Canada: collaborator

Study Sites (1)

Clinical Physiology Research Laboratory

Edmonton, Alberta, T6G 2J1, Canada

Location

Related Publications (4)

• Tedjasaputra V, Bryan TL, van Diepen S, Moore LE, Bouwsema MM, Welsh RC, Petersen SR, Stickland MK. Dopamine receptor blockade improves pulmonary gas exchange but decreases exercise performance in healthy humans. J Physiol. 2015 Jul 15;593(14):3147-57. doi: 10.1113/JP270238. Epub 2015 Jun 8.

  PMID: 25952760 BACKGROUND

• ROUGHTON FJ, FORSTER RE. Relative importance of diffusion and chemical reaction rates in determining rate of exchange of gases in the human lung, with special reference to true diffusing capacity of pulmonary membrane and volume of blood in the lung capillaries. J Appl Physiol. 1957 Sep;11(2):290-302. doi: 10.1152/jappl.1957.11.2.290. No abstract available.

  PMID: 13475180 BACKGROUND

• Argiento P, Chesler N, Mule M, D'Alto M, Bossone E, Unger P, Naeije R. Exercise stress echocardiography for the study of the pulmonary circulation. Eur Respir J. 2010 Jun;35(6):1273-8. doi: 10.1183/09031936.00076009. Epub 2009 Nov 19.

  PMID: 19926746 BACKGROUND

• Bryan TL, van Diepen S, Bhutani M, Shanks M, Welsh RC, Stickland MK. The effects of dobutamine and dopamine on intrapulmonary shunt and gas exchange in healthy humans. J Appl Physiol (1985). 2012 Aug 15;113(4):541-8. doi: 10.1152/japplphysiol.00404.2012. Epub 2012 Jun 14.

  PMID: 22700799 BACKGROUND

MeSH Terms

Interventions

Dopamine; Metoclopramide; RE1-silencing transcription factor

Intervention Hierarchy (Ancestors)

Biogenic Monoamines; Biogenic Amines; Amines; Organic Chemicals; Catecholamines; Catechols; Phenols; Benzene Derivatives; Hydrocarbons, Aromatic; Hydrocarbons, Cyclic; Hydrocarbons; Benzamides; Amides; para-Aminobenzoates; Aminobenzoates; Benzoates; Acids, Carbocyclic; Carboxylic Acids; Chlorobenzoates; Hydroxybenzoate Ethers; Hydroxybenzoates; Hydroxy Acids; Phenyl Ethers

Study Officials

• Michael K Stickland, PhD

  University of Alberta

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 10, 2016
• First Posted: November 17, 2016
• Study Start: December 1, 2016
• Primary Completion: June 1, 2025
• Study Completion: June 1, 2025
• Last Updated: July 23, 2025

Record last verified: 2025-07

Locations

CA (1)