NCT02932852

Brief Summary

Transplantation of cellularized human cornea impregnated and populated by mesenchymal stem cells derived from the patient's adipose tissue. The purpose of the study is to assess the safety, tolerability, and preliminary efficacy of transplantation of a single dose of autologous mesenchymal adipose tissue derived adult stem cells (ADAS) cellularized into laminas for subjects with corneal defects. 3 groups will be included in the study: (1) transplantation of ADAS alone without scaffold, (2) transplantation of scaffold (human corneal decellularized lamina) without ADAS, and (3) transplantation of ADAS cellularized on scaffold (the human corneal decellularized lamina)

Trial Health

55
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
12

participants targeted

Target at P25-P50 for early_phase_1

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2015

Completed
11 months until next milestone

First Submitted

Initial submission to the registry

October 2, 2016

Completed
11 days until next milestone

First Posted

Study publicly available on registry

October 13, 2016

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 1, 2017

Completed
Last Updated

October 13, 2016

Status Verified

October 1, 2016

Enrollment Period

1.4 years

First QC Date

October 2, 2016

Last Update Submit

October 11, 2016

Conditions

Keywords

stem cellmesenchymalADASctransplantationcorneacorneal defectskeratoconusscaffoldlamina

Outcome Measures

Primary Outcomes (1)

  • Vision recovery (Corrected Distance Visual Acuity)

    The Best Corrected Visual Acuity will be measured in each postoperative visit to control any important decrease relative to the surgery

    post operative visit at 1 day, 1 month, 3 months and 6 months

Secondary Outcomes (5)

  • Corneal Thickness

    post operative visit at 1 day, 1 month, 3 months and 6 months

  • Topopgraphy

    post operative visit at 1 month, 3 months and 6 months

  • Anterior segment Optical Coherence Tomography

    post operative visit at 1 month, 3 months and 6 months

  • Slit Lamp Observation

    post operative visit at 1 day, 1 month, 3 months and 6 months

  • refraction measurement

    post operative visit at 1 day, 1 month, 3 months and 6 months

Study Arms (3)

GROUP I

ACTIVE COMPARATOR

lipoaspiration and transplantation of ADAS alone without scaffold

Procedure: LipoaspirationProcedure: Transplantation

GROUP II

ACTIVE COMPARATOR

Lipoaspiration and transplantation of scaffold (human corneal decellularized lamina) without ADAS

Procedure: LipoaspirationProcedure: Transplantation

GROUP III

ACTIVE COMPARATOR

Lipoaspiration and transplantation of ADAS cellularized on scaffold (the human corneal decellularized lamina)

Procedure: LipoaspirationProcedure: Transplantation

Interventions

The patient will have a liposuction surgery from which the autologous ADAS cell to transplant in the cornea will be obtained.

GROUP IGROUP IIGROUP III

The ophthalmologist will carry out the transplantation of the ADAS cells with or without scaffold in the intrastromal cornea of the patient

GROUP IGROUP IIGROUP III

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Patients affected by corneal stromas dystrophies of any type, but particularly keratoconus, showing clear evidence at the ophthalmic examination of the presence and clear expression of the disease
  • Age : ≥ 18 years
  • Gender: any
  • Willing to collaborate and to attend to the clinical follow ups for the next five years
  • Patients willing to sign informed consent
  • Able and willing to comply with all study requirements
  • Patients with and no worse than 0.6 for the better vision patients
  • Comprehensive clinical ophthalmological including slit lamp photography of the cornea of either eye
  • Corneal topography map including both anterior and posterior corneal surfaces
  • Specular microscopy
  • Confocal microscopy of the centre of the cornea
  • Absence of anterior segment inflammation
  • Medically suitable to undergo corneal graft surgery with local anesthesia
  • Medically suitable for general anesthesia or waking sedation, if needed
  • Normal serum chemistry and hematology screening tests
  • +6 more criteria

You may not qualify if:

  • \- Patients not willing to sign informed consent
  • Defects in corneal transparency with a potential to affect the visual outcome
  • Vision of 0.05 or less
  • Severe corneal deformation
  • Infection
  • Moderate or severe dry eye
  • Chronic ocular surface inflammation of any origin
  • Previous ocular surgery other than cataract
  • Presence of cataract or other media opacity that would influence ocular fundus documentation and adequate ERG and visual field evaluation.
  • Other ophthalmic comorbidity like glaucoma and uveitis
  • Known allergy: example to propacaine, ciprofloxacin, fluorescein , indocyanine green, or others to specify
  • Known coagulation abnormalities
  • Any medical condition likely interferes to cause serious adverse events during the study
  • Presence of active or inactive choroidal neovascularization (CNV) in the eye to be treated
  • History of malignancy
  • +10 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Optica General

Saida, 10450, Lebanon

RECRUITING

Related Publications (5)

  • Alio del Barrio JL, Chiesa M, Garagorri N, Garcia-Urquia N, Fernandez-Delgado J, Bataille L, Rodriguez A, Arnalich-Montiel F, Zarnowski T, Alvarez de Toledo JP, Alio JL, De Miguel MP. Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model. Exp Eye Res. 2015 Mar;132:91-100. doi: 10.1016/j.exer.2015.01.020. Epub 2015 Jan 24.

    PMID: 25625506BACKGROUND
  • De Miguel MP, Alio JL, Arnalich-Montiel F, Fuentes-Julian S, de Benito-Llopis L, Amparo F, Bataille L. Cornea and ocular surface treatment. Curr Stem Cell Res Ther. 2010 Jun;5(2):195-204. doi: 10.2174/157488810791268663.

    PMID: 19941445BACKGROUND
  • Alio del Barrio JL, Chiesa M, Gallego Ferrer G, Garagorri N, Briz N, Fernandez-Delgado J, Sancho-Tello Valls M, Botella CC, Garcia-Tunon I, Bataille L, Rodriguez A, Arnalich-Montiel F, Gomez Ribelles JL, Antolinos-Turpin CM, Gomez-Tejedor JA, Alio JL, De Miguel MP. Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model. J Biomed Mater Res A. 2015 Mar;103(3):1106-18. doi: 10.1002/jbm.a.35249. Epub 2014 Jun 18.

    PMID: 24910285BACKGROUND
  • Alio JL. Corneal regeneration: How can we make further progress in corneal surgery with advanced therapies to avoid corneal substitution: ESCRS Binkhorst Medal Lecture 2023. J Cataract Refract Surg. 2025 Nov 1;51(11):1026-1033. doi: 10.1097/j.jcrs.0000000000001745.

  • El Zarif M, Alio JL, Alio Del Barrio JL, Abdul Jawad K, Palazon-Bru A, Abdul Jawad Z, De Miguel MP, Makdissy N. Corneal Stromal Regeneration Therapy for Advanced Keratoconus: Long-term Outcomes at 3 Years. Cornea. 2021 Jun 1;40(6):741-754. doi: 10.1097/ICO.0000000000002646.

MeSH Terms

Conditions

Corneal Dystrophies, HereditaryKeratoconusCorneal Diseases

Interventions

Transplantation

Condition Hierarchy (Ancestors)

Eye DiseasesEye Diseases, HereditaryGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and Abnormalities

Intervention Hierarchy (Ancestors)

Surgical Procedures, Operative

Study Officials

  • Mona El Zarif, OD

    Optica General

    STUDY CHAIR

Central Study Contacts

Jorge Alio Sanz, MD, PhD

CONTACT

Nehman Makdissy, PhD

CONTACT

Study Design

Study Type
interventional
Phase
early phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

October 2, 2016

First Posted

October 13, 2016

Study Start

November 1, 2015

Primary Completion

April 1, 2017

Last Updated

October 13, 2016

Record last verified: 2016-10

Locations