Link Between Plasma Citrulline and Lipopolysaccharide Concentrations in the Critically Ill

NCT02902276 | Unknown

• 1 other identifier: P/2016/187
• Study Type: observational
• Target: 120
• Locations: 1 country
• Sites: 1

Brief Summary

In critically ill patients, the small bowel permeability is increased, leading to bacterial translocation, and systemic inflammatory response syndrome. Plasma citrulline is a validated biomarker of functional enterocyte mass. Lipopolysaccharide (LPS) is a part of Gram negative bacteria, and plasma LPS concentration is a biomarker of bacterial translocation. The link between plasma citrulline and LPS in the critically ill is unknown. Investigators hypothesize that patients presenting with low plasma citrulline concentration, with presumed altered small bowel function, would have an increased phenomenon of bacterial translocation, and increased plasma LPS concentration. Investigators aimed to evaluate the link between plasma citrulline and LPS concentrations in critically ill patients at the time of ICU admission.

Conditions

Critically Ill

Keywords

plasma citrulline; lipopolysaccharide; critically ill; Hospitalization in an Intensive Care Unit

Outcome Measures

Primary Outcomes (2)

• Plasma citrulline concentration

  biological assay

  Up to 12 hours after admission in the unit

• plasma lipopolysaccharide concentration

  biological assay

  Up to 12 hours after admission in the unit

Interventions

additional blood sample OTHER

Two blood samples will be taken at ICU admission

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Critically ill patients requiring ICU admission for at least 48 hours, without chronic small bowel disease, without chronic renal failure (usual clearance of creatinine \< 50 mL/min), not pregnant.

You may qualify if:

• Critically ill patients requiring ICU admission for at least 48 hours
• Adults

You may not qualify if:

• Chronic small bowel disease
• Chronic renal failure (clearance of creatinine \< 50 mL/min)
• Pregnancy

Sponsors & Collaborators

• Centre Hospitalier Universitaire de Besancon: lead

Study Sites (1)

Centre Hospitalier Universitaire de Besançon

Besançon, 25030, France

RECRUITING

Related Publications (1)

• Chaignat C, Lagrost L, Moretto K, de Barros JP, Winiszewski H, Grober J, Saas P, Piton G. Plasma citrulline concentration and plasma LPS detection among critically ill patients a prospective observational study. J Crit Care. 2024 Feb;79:154438. doi: 10.1016/j.jcrc.2023.154438. Epub 2023 Oct 3.

  PMID: 37797404 DERIVED

Biospecimen

Retention: SAMPLES WITHOUT DNA

2000 microliters of plasma will be stored at -80°C for future analyses (lipopolysaccharide concentration, cytokines).

MeSH Terms

Conditions

Critical Illness

Condition Hierarchy (Ancestors)

Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• Gaël Piton, MD, PhD

  CHU Besançon

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Gaël Piton, MD, PhD

CONTACT

0033381668224; gpiton@chu-besancon.fr

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: August 11, 2016
• First Posted: September 15, 2016
• Study Start: July 1, 2016
• Primary Completion: February 1, 2017
• Study Completion: February 1, 2017
• Last Updated: September 15, 2016

Record last verified: 2016-09

Data Sharing

• IPD Sharing: Will not share

Locations

FR (1)