NCT02826759

Brief Summary

This study is designed to compare the serum sphingolipidomic analyses in healthy, pre-diabetic and diabetic subjects. age, sex and BMI are matched among these three groups. As ceramide, sphingosine, sphingosine-1-phosphate and sphinganine are involved in inflammation, immunity and cancer, investigators proposed a hypothesis that sphingosine-1-phosphate and other sphingolipids may be associated with the progress of type 2 diabetes. sphingolipids may be a biomarker for diabetes.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
200

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Jul 2016

Shorter than P25 for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

July 1, 2016

Completed
5 days until next milestone

First Submitted

Initial submission to the registry

July 6, 2016

Completed
5 days until next milestone

First Posted

Study publicly available on registry

July 11, 2016

Completed
4 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2016

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2016

Completed
Last Updated

July 11, 2016

Status Verified

April 1, 2016

Enrollment Period

4 months

First QC Date

July 6, 2016

Last Update Submit

July 7, 2016

Conditions

Keywords

sphingolipiddiabetes mellitusbiomarkerobesityinsulin resistance

Outcome Measures

Primary Outcomes (1)

  • serum concentration of sphingosine-1-phosphate, micromol per liter

    Nov, 2016

Secondary Outcomes (4)

  • serum concentration of sphingosine, micromol per liter

    Nov, 2016

  • serum concentration of sphinganine-1-phosphate, micromol per liter

    Nov, 2016

  • serum concentration of sphinganine, micromol per liter

    Nov, 2016

  • serum concentration of ceramide, micromol per liter

    Nov, 2016

Study Arms (5)

serum sphingolipid metabolites in healthy subjects

Healthy subjects are classified according to BMI into three subgroups: healthy normal weight subjects, healthy overweight subjects and healthy subjects with obesity. Age and sex are matched among three subgroups. serum sphingolipid metabolites including sphingosine-1-phosphate will be compared.

serum sphingolipid metabolites in diabetic subjects

Diagnosed diabetic patients are divided into three subgroups: diabetic patients with normal weight, overweight and obesity. age and sex are matched.

Other: diabetes

serum sphingolipid metabolites in the progression of diabetes

serum sphingolipid metabolites are compared among three age-, sex- and body mass index-matched subgroups: healthy subjects, subjects with pre-diabetes, subjects with diabetes

Other: diabetes

serum sphingolipid metabolites and HbA1c

diabetic subjects are divided by HbA1c level. the cut-offs are 7% and 9%. serum sphingolipid metabolites will be tested among diabetic patients with HbA1c \<7%, 7%-9% and \>9%

Other: diabetes

serum sphingolipid metabolites in insulin-resistant subjects

comparison of serum sphingolipid metabolites in newly diagnosed insulin-resistant subjects and age-, sex- and BMI-matched healthy controls.

Other: diabetes

Interventions

the exposure of interests are obesity, diabetes and insulin-resistance

Also known as: insulin-resistance
serum sphingolipid metabolites and HbA1cserum sphingolipid metabolites in diabetic subjectsserum sphingolipid metabolites in insulin-resistant subjectsserum sphingolipid metabolites in the progression of diabetes

Eligibility Criteria

Age18 Years - 90 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

subjects undergoing oral glucose tolerance test and hospitalized type 2 diabetic patients in the Dpartment of Endocrinology in the First Affiliated Hospital of Xi'an Jiaotong Unversity are collected. healthy subjects undergoing routine physical exam in the department of medical examination center, First Affiliated Hospital of Xi'an Jiaotong Unversity are recruited

You may qualify if:

  • clinical diagnosis of diabetes mellitus, type 2
  • clinical diagnosis of prediabetic status
  • older than 18 and younger than 90 years-old
  • clinical diagnosis of insulin-resistance
  • clinical diagnosis of newly onset of diabetes mellitus, type 2
  • those who are willing to participate in the trial and sign the consent form

You may not qualify if:

  • any cardiovascular disease (myocardial infarction, heart failure, cerebrovascular accident)
  • missing information of BMI
  • sever liver, kidney dysfunction
  • sever pancreatitis or those who received pancreatectomy
  • on medications of hormone, immunosuppressive therapy or drugs that may affect the bioactivity or concentration of sphingolipids (e.g. asprin )
  • patients on pregnancy
  • sever systematic diseases including carcinoma, mental disorder, sever anemia et al.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

The First Affiliated Hospital of Xi'an Jiaotong University

Xi'an, Shaanxi, 710061, China

Location

Related Publications (1)

  • Sui J, He M, Wang Y, Zhao X, He Y, Shi B. Sphingolipid metabolism in type 2 diabetes and associated cardiovascular complications. Exp Ther Med. 2019 Nov;18(5):3603-3614. doi: 10.3892/etm.2019.7981. Epub 2019 Sep 6.

Biospecimen

Retention: SAMPLES WITHOUT DNA

serum samples are used to test the concentration of sphingolipid metabolites ( ceramide, sphingosine, sphingosine-1-phosphate, sphinganine, sphinganine-1-phosphate) by mass spectrometry

MeSH Terms

Conditions

Diabetes Mellitus, Type 2Prediabetic StateDiabetes MellitusObesityInsulin Resistance

Interventions

insulin resistance factor (uremia)

Condition Hierarchy (Ancestors)

Glucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic DiseasesEndocrine System DiseasesOverweightOvernutritionNutrition DisordersBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsHyperinsulinism

Study Officials

  • Jing Sui

    First Affiliated Hospital Xi'an Jiaotong University

    STUDY DIRECTOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 6, 2016

First Posted

July 11, 2016

Study Start

July 1, 2016

Primary Completion

November 1, 2016

Study Completion

November 1, 2016

Last Updated

July 11, 2016

Record last verified: 2016-04

Locations