Frequency Of MCP-1 And CCR2 Gene Polymorphisms And Its Effect On Gene Expression In Patients With AgP

NCT02817568 | Completed

• 3 other identifiers: 11102024SU1985SUPeriodontology
• Study Type: observational
• Target: 215
• Locations: 0 countries
• Sites: N/A

Brief Summary

The aim of this study is to estimate genetic impact of MCP-1 -2518 and its receptor CCR2 -190 polymorphisms on AgP patients among Turkish individuals and whether MCP-1 genotype effects mRNA levels of Peripheral Blood Mononuclear Cell Leukocyte (PBML)

Conditions

Aggressive Periodontitis

Keywords

Aggressive Periodontitis; C-C chemokine receptor 2; Gene expression; monocyte chemoattractant protein-1,; restriction fragment length polymorphism

Outcome Measures

Primary Outcomes (1)

• Frequency of MCP-1 -2518 A/G and CCR2 -190 G/A gene polymorphisms in Aggressive Periodontitis

  one year

Secondary Outcomes (1)

• MCP-1 gene expression in AgP patients

  six months

Study Arms (2)

Aggressive Periodontitis (case)

Single intervention has been performed for each subject. Peripheral blood sample was obtained in conjunction with clinical measurements in this intervention.(Drawing Blood)

Other: Drawing Blood

Healthy (Control)

Single intervention has been performed for each subject. Peripheral blood sample was obtained in conjunction with clinical measurements in this intervention. (Drawing Blood)

Other: Drawing Blood

Interventions

Drawing Blood OTHER

Drawing Blood for genetic analysis

Aggressive Periodontitis (case); Healthy (Control)

Eligibility Criteria

• Age: 16 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

This cross-sectional case control study was carried out at Selcuk University, Faculty of Dentistry, Department of Periodontology. The control group included periodontally healthy volunteers from staff and other subjects referring to the School of Dentistry.

You may qualify if:

• Periodontally H control group had \< 3mm PD, \< 2 GI and no signs of interproximal attachment loss and a history of periodontal disease.
• The AgP group included individuals diagnosed with localized AgP (LAgP) or generalized AgP (GAgP) who were otherwise healthy. Periodontal attachment loss ≥4 mm not involving more than two permanent teeth, other than the first molars and incisors were diagnosed with LAgP (n: 43); patients with involvement of at least three teeth, other than the first molars and incisors with an attachment loss ≥4 mm were diagnosed with GAgP

You may not qualify if:

• Patients who have any systemic diseases or conditions that could effect the periodontium
• hepatitis and/or immunodeficiency virus infection
• a history of periodontal treatment and antibiotic therapy within the 6 months
• \< 16 teeth in their mouth
• Subjects who had smoked and were ongoing orthodontic treatment were excluded.

Sponsors & Collaborators

• Abant Izzet Baysal University: lead
• Selcuk University: collaborator

Related Publications (6)

• Armitage GC. Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000. 2004;34:9-21. doi: 10.1046/j.0906-6713.2002.003421.x. No abstract available.

  PMID: 14717852 BACKGROUND

• Yu X, Graves DT. Fibroblasts, mononuclear phagocytes, and endothelial cells express monocyte chemoattractant protein-1 (MCP-1) in inflamed human gingiva. J Periodontol. 1995 Jan;66(1):80-8. doi: 10.1902/jop.1995.66.1.80.

  PMID: 7891256 RESULT

• Zhu XL, Meng HX, Zhang L, Xu L, Chen ZB, Shi D, Feng XH, Zhang X. Association analysis between the -2518MCP-1(A/G) polymorphism and generalized aggressive periodontitis in a Chinese population. J Periodontal Res. 2012 Jun;47(3):286-92. doi: 10.1111/j.1600-0765.2011.01426.x. Epub 2011 Nov 27.

  PMID: 22117820 RESULT

• Pradeep AR, Daisy H, Hadge P. Gingival crevicular fluid levels of monocyte chemoattractant protein-1 in periodontal health and disease. Arch Oral Biol. 2009 May;54(5):503-9. doi: 10.1016/j.archoralbio.2009.02.007. Epub 2009 Mar 16.

  PMID: 19286166 RESULT

• Emingil G, Atilla G, Huseyinov A. Gingival crevicular fluid monocyte chemoattractant protein-1 and RANTES levels in patients with generalized aggressive periodontitis. J Clin Periodontol. 2004 Oct;31(10):829-34. doi: 10.1111/j.1600-051X.2004.00584.x.

  PMID: 15367184 RESULT

• Kurtis B, Tuter G, Serdar M, Akdemir P, Uygur C, Firatli E, Bal B. Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis. J Periodontol. 2005 Nov;76(11):1849-55. doi: 10.1902/jop.2005.76.11.1849.

  PMID: 16274303 RESULT

Biospecimen

Retention: SAMPLES WITH DNA

Peripheral Blood of each subject is kept -80 celcius for further analysis.

MeSH Terms

Conditions

Aggressive Periodontitis

Condition Hierarchy (Ancestors)

Periodontitis; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Officials

• Nilgun O Alptekin, Phd, DDS

  Baskent University, Faculty of Dentistry, Periodontology Department

  PRINCIPAL INVESTIGATOR

• Sadiye Gunpinar, Phd, DDS

  Abant Izzet Baysal University, Faculty of Dentistry, Periodontology Department

  PRINCIPAL INVESTIGATOR

• Hasan Acar, Phd

  Selcuk University, Faculty of Medicine, Department of Genetics

  PRINCIPAL INVESTIGATOR

• Vasfiye B Ucar, Phd

  Selcuk University, Faculty of Medicine, Department of Genetics

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Abant Izzet Baysal University, Faculty of Dentistry

Study Record Dates

• First Submitted: June 27, 2016
• First Posted: June 29, 2016
• Study Start: March 1, 2011
• Primary Completion: August 1, 2012
• Study Completion: August 1, 2014
• Last Updated: June 30, 2016

Record last verified: 2016-06

Data Sharing

• IPD Sharing: Will not share