NCT02812290

Brief Summary

Objective: To evaluate the potential impact of molecular phenotyping of transbronchial biopsies in lung transplant recipients with allograft dysfunction, and the potential for developing a safer endobronchial mucosal biopsy format.

Trial Health

83
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
700

participants targeted

Target at P75+ for all trials

Timeline
7mo left

Started May 2016

Longer than P75 for all trials

Geographic Reach
5 countries

10 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress95%
May 2016Dec 2026

Study Start

First participant enrolled

May 1, 2016

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

May 31, 2016

Completed
24 days until next milestone

First Posted

Study publicly available on registry

June 24, 2016

Completed
9.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2026

Expected
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2026

Last Updated

November 20, 2025

Status Verified

November 1, 2025

Enrollment Period

10.1 years

First QC Date

May 31, 2016

Last Update Submit

November 17, 2025

Conditions

Keywords

antibody mediated rejectionT cell mediated rejection

Outcome Measures

Primary Outcomes (2)

  • Report the molecular scores (probability) of lung transplant disease in a reference set of 600 transbronchial biopsies.

    Molecular classifier predicts antibody mediated and T cell mediated rejection, and chronic allograft dysfunction.

    two years

  • Report the molecular diagnoses of the MMDx-TBB system

    Compare MMDx readings to standard-of-care TBB histology and clinical diagnosis of CLAD.

    two years

Secondary Outcomes (3)

  • Report the molecular scores (probability) of lung transplant disease in a reference set of 600 mucosal endobronchial biopsies.

    two years

  • Report the molecular diagnoses of the MMDx-3BMB system

    two years

  • Report the molecular changes over time in medically indicated follow-up biopsies

    two years

Interventions

In the second phase of the study, two biopsy bites from the same patient will be collected to assess tissue sampling variability.

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

Patients with functioning lung transplant biopsied to determine their graft dysfunction or to confirm good function as per standard of care.

You may qualify if:

  • All lung transplant recipients undergoing a biopsy as determined by their surgeon or physician.

You may not qualify if:

  • Patients who declined participation or unable to give informed consent.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (11)

St. Joseph's Hospital and Medical Center 350 West Thomas Road, Floor 8HLT

Phoenix, Arizona, 85013, United States

RECRUITING

University of Maryland School of Medicine

Baltimore, Maryland, 21201, United States

COMPLETED

Division of Pulmonary and Critical Care, Washington University School of Medicine

St Louis, Missouri, 63110, United States

COMPLETED

University of Texas at San Antonio

San Antonio, Texas, 21201, United States

COMPLETED

The Alfred Hospital, Monash University

Melbourne, Australia

COMPLETED

Department of Thoracic Surgery, Medical University of Vienna

Vienna, Austria

COMPLETED

Alberta Transplant Applied Genomics Centre, University of Alberta

Edmonton, Alberta, T6G 2E1, Canada

RECRUITING

Department of Medicine, University of Alberta

Edmonton, Alberta, T6G 2G3, Canada

RECRUITING

University Health Network, Toronto General Hospital

Toronto, Ontario, M5G 2C4, Canada

COMPLETED

Charles University/Hospital Motol

Prague, Czechia

RECRUITING

Thoracic Surgery Transplant Clinic

Szczecin, 70891, Poland

COMPLETED

Related Publications (9)

  • Madill-Thomsen KS, Halloran PF. Precision diagnostics in transplanted organs using microarray-assessed gene expression: concepts and technical methods of the Molecular Microscope(R) Diagnostic System (MMDx). Clin Sci (Lond). 2024 Jun 5;138(11):663-685. doi: 10.1042/CS20220530.

    PMID: 38819301BACKGROUND
  • Halloran KM, Parkes MD, Chang J, Timofte IL, Snell GI, Westall GP, Hachem R, Kreisel D, Trulock E, Roux A, Juvet S, Keshavjee S, Jaksch P, Klepetko W, Halloran PF. Molecular assessment of rejection and injury in lung transplant biopsies. J Heart Lung Transplant. 2019 May;38(5):504-513. doi: 10.1016/j.healun.2019.01.1317. Epub 2019 Feb 6.

  • Halloran K, Parkes MD, Timofte I, Snell G, Westall G, Havlin J, Lischke R, Hachem R, Kreisel D, Levine D, Kubisa B, Piotrowska M, Juvet S, Keshavjee S, Jaksch P, Klepetko W, Hirji A, Weinkauf J, Halloran PF. Molecular T-cell-mediated rejection in transbronchial and mucosal lung transplant biopsies is associated with future risk of graft loss. J Heart Lung Transplant. 2020 Dec;39(12):1327-1337. doi: 10.1016/j.healun.2020.08.013. Epub 2020 Aug 26.

  • Halloran K, Parkes MD, Timofte IL, Snell GI, Westall GP, Hachem R, Kreisel D, Levine D, Juvet S, Keshavjee S, Jaksch P, Klepetko W, Hirji A, Weinkauf J, Halloran PF. Molecular phenotyping of rejection-related changes in mucosal biopsies from lung transplants. Am J Transplant. 2020 Apr;20(4):954-966. doi: 10.1111/ajt.15685. Epub 2019 Dec 1.

  • Parkes MD, Halloran K, Hirji A, Pon S, Weinkauf J, Timofte IL, Snell GI, Westall GP, Havlin J, Lischke R, Zajacova A, Hachem R, Kreisel D, Levine D, Kubisa B, Piotrowska M, Juvet S, Keshavjee S, Jaksch P, Klepetko W, Halloran PF. Transcripts associated with chronic lung allograft dysfunction in transbronchial biopsies of lung transplants. Am J Transplant. 2022 Apr;22(4):1054-1072. doi: 10.1111/ajt.16895. Epub 2021 Dec 16.

  • Halloran K, Mackova M, Parkes MD, Hirji A, Weinkauf J, Timofte IL, Snell GI, Westall GP, Lischke R, Zajacova A, Havlin J, Hachem R, Kreisel D, Levine D, Kubisa B, Piotrowska M, Juvet S, Keshavjee S, Jaksch P, Klepetko W, Halloran PF. The molecular features of chronic lung allograft dysfunction in lung transplant airway mucosa. J Heart Lung Transplant. 2022 Dec;41(12):1689-1699. doi: 10.1016/j.healun.2022.08.014. Epub 2022 Aug 27.

  • Gauthier PT, Mackova M, Hirji A, Weinkauf J, Timofte IL, Snell GI, Westall GP, Havlin J, Lischke R, Zajacova A, Simonek J, Hachem R, Kreisel D, Levine D, Kubisa B, Piotrowska M, Juvet S, Keshavjee S, Jaksch P, Klepetko W, Halloran K, Halloran PF. Defining a natural killer cell-enriched molecular rejection-like state in lung transplant transbronchial biopsies. Am J Transplant. 2023 Dec;23(12):1922-1938. doi: 10.1016/j.ajt.2023.06.003. Epub 2023 Jun 7.

  • Mackova M, Gauthier P, Chang J, Hirji A, Weinkauf J, Juvet S, Keshavjee S, Havlin J, Lischke R, Zajacova A, Snell G, Westall G, Halloran PF, Halloran K. Molecular biopsy features associated with baseline lung allograft dysfunction in a multicenter international cohort. J Heart Lung Transplant. 2025 Nov 14:S1053-2498(25)02394-0. doi: 10.1016/j.healun.2025.11.005. Online ahead of print.

  • Halloran PF. Integrating molecular and histologic interpretation of transplant biopsies. Clin Transplant. 2021 Apr;35(4):e14244. doi: 10.1111/ctr.14244. Epub 2021 Feb 17. No abstract available.

Biospecimen

Retention: SAMPLES WITHOUT DNA

Lung transplant biopsies taken as per local standard of care.

Study Officials

  • Philip F Halloran, MD, PhD

    Faculty of Medicine and Dentistry, University of Alberta

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Konrad S Famulski, PhD

CONTACT

Robert Polakowski, PhD

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Distinguished Professor

Study Record Dates

First Submitted

May 31, 2016

First Posted

June 24, 2016

Study Start

May 1, 2016

Primary Completion (Estimated)

June 1, 2026

Study Completion (Estimated)

December 1, 2026

Last Updated

November 20, 2025

Record last verified: 2025-11

Locations