NCT02797522

Brief Summary

Normal healthy volunteer (NHV) participants will enroll sequentially into a total of 6 escalating dose levels (6 subjects per dose level), randomized to receive a single dose of ARC-521 Injection or placebo. The maximum study duration for NHVs is approximately 21 weeks. Hepatitis B e Antigen (HBeAg)-negative participants with (CHB) will enroll sequentially into 3 dose levels (8 patients per dose level) to receive multiple doses of open label ARC-521 Injection. For each CHB participant the maximum study duration is approximately 37 weeks.

Trial Health

57
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
47

participants targeted

Target at P50-P75 for phase_1

Timeline
Completed

Started Jun 2016

Shorter than P25 for phase_1

Geographic Reach
1 country

1 active site

Status
terminated

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2016

Completed
7 days until next milestone

First Submitted

Initial submission to the registry

June 8, 2016

Completed
5 days until next milestone

First Posted

Study publicly available on registry

June 13, 2016

Completed
5 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2016

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2016

Completed
1.9 years until next milestone

Results Posted

Study results publicly available

September 25, 2018

Completed
Last Updated

January 12, 2026

Status Verified

December 1, 2025

Enrollment Period

5 months

First QC Date

June 8, 2016

Results QC Date

January 10, 2018

Last Update Submit

December 18, 2025

Conditions

Outcome Measures

Primary Outcomes (11)

  • Number of Participants With Treatment-Emergent Adverse Events (TEAEs): Healthy Volunteers

    An adverse event (AE) is any untoward medical occurrence which does not necessarily have a causal relationship with this treatment. A serious AE is any AE that: results in death; is life-threatening; requires inpatient hospitalization or prolongation of an existing hospitalization; results in persistent or significant disability/incapacity; is a congenital anomaly/birth defect; or is a medically important event or reaction.Events were categorized as mild, moderate or severe. TEAEs were defined as all AEs starting or worsening after commencement of treatment with investigational product. A treatment-related TEAE was one whose relationship to treatment was noted as unlikely, possibly, or probably related.

    From first dose of study drug through Day 29 (± 1 day)

  • Number of Participants With TEAEs: CHB Participants

    An AE is any untoward medical occurrence which does not necessarily have a causal relationship with this treatment. A serious AE is any AE that: results in death; is life-threatening; requires inpatient hospitalization or prolongation of an existing hospitalization; results in persistent or significant disability/incapacity; is a congenital anomaly/birth defect; or is a medically important event or reaction.Events were categorized as mild, moderate or severe. TEAEs were defined as all AEs starting or worsening after commencement of treatment with investigational product. A treatment-related TEAE was one whose relationship to treatment was noted as unlikely, possibly, or probably related.

    From first dose of study drug through Day 142 (± 3 days)

  • Pharmacokinetics of ARC-521 Injection: Area Under the Plasma-Concentration-Time Curve From Time 0-24 Hours (AUC0-24), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Area Under the Plasma-Concentration-Time Curve From Time 0 to the Last Quantifiable Plasma Concentration (AUClast), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Area Under the Plasma-Concentration-Time Curve From Time 0 Extrapolated to Infinity (AUCinf), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Maximum Observed Plasma Concentration (Cmax), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Clearance (CL), Healthy Volunteers

    Through 48 hrs post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Apparent Volume of Distribution (V), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Terminal Elimination Rate Constant (Kel), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Pharmacokinetics of ARC-521 Injection: Terminal Elimination Half-Life (t1/2), Healthy Volunteers

    Through 48 hours post-dose on Day 1

  • Change Over Time in Viral Antigens and DNA in CHB Participants as a Measure of Activity of ARC-521 Injection

    Baseline to Day 142

Secondary Outcomes (2)

  • Change Over Time in Cytokine Levels After Single and Multiple Doses of ARC-521 Injection

    Through 24 hours post-dose (Day 1 for NHVs, and Days 1, 29 & 57 for CHB participants)

  • Change Over Time in Complement Levels After Single and Multiple Doses of ARC-521 Injection

    Through 24 hours post-dose (Day 1 for NHVs, and Days 1, 29 & 57 for CHB participants)

Study Arms (11)

NHV Participants: Cohort 1

EXPERIMENTAL

NHV participants administered a single dose of ARC-521 Injection at a dose of 0.6 mg/kg.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

NHV Participants: Cohort 2

EXPERIMENTAL

NHV participants administered a single dose of ARC-521 Injection at a dose of 1 mg/kg.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

NHV Participants: Cohort 3

EXPERIMENTAL

NHV participants administered a single dose of ARC-521 Injection at a dose of 2 mg/kg.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

NHV Participants: Cohort 4

EXPERIMENTAL

NHV participants administered a single dose of ARC-521 Injection at a dose of 4 mg/kg.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

NHV Participants: Cohort 5

EXPERIMENTAL

NHV participants administered a single dose of ARC-521 Injection at a dose of 5 mg/kg.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

NHV Participants: Cohort 6

EXPERIMENTAL

NHV participants administered a single dose of ARC-521 Injection at a dose of 6 mg/kg.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

NHV Participants: Placebo

PLACEBO COMPARATOR

NHV participants administered 0.9% normal saline to match ARC-521 Injection at doses of 0.6, 1, 2, 4, 5 and 6 mg/kg.

Other: PlaceboDrug: antihistamineDrug: acetaminophen

CHB Participants: Cohort 3b

EXPERIMENTAL

Treatment-naive participants with CHB administered 3 doses of ARC-521 Injection at 2 mg/kg once every 4 weeks. Participants are treatment-naive if they have not been on continual nucleoside analog (NUC) therapy (any NUC) for at least 6 months prior to screening (or have never been on NUCs).

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

CHB Participants: Cohort 4b

EXPERIMENTAL

Treatment-naive participants with CHB administered 3 doses of ARC-521 Injection at 4 mg/kg once every 4 weeks. Participants are treatment-naive if they have not been on continual NUC therapy (any NUC) for at least 6 months prior to screening (or have never been on NUCs).

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophen

CHB Participants: Cohort 3c

EXPERIMENTAL

Participants with CHB currently on NUCs (entecavir or tenofovir for at least 6 months) administered 3 doses of ARC-521 Injection at 2 mg/kg once every 4 weeks.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophenDrug: entecavirDrug: tenofovir

CHB Participants: Cohort 4c

EXPERIMENTAL

Participants with CHB currently on NUCs (entecavir or tenofovir for at least 6 months) administered 3 doses of ARC-521 Injection at 4 mg/kg once every 4 weeks.

Drug: ARC-521 InjectionDrug: antihistamineDrug: acetaminophenDrug: entecavirDrug: tenofovir

Interventions

CHB Participants: Cohort 3bCHB Participants: Cohort 3cCHB Participants: Cohort 4bCHB Participants: Cohort 4cNHV Participants: Cohort 1NHV Participants: Cohort 2NHV Participants: Cohort 3NHV Participants: Cohort 4NHV Participants: Cohort 5NHV Participants: Cohort 6
PlaceboOTHER

0.9% normal saline

NHV Participants: Placebo

Approximately two hours prior to ARC-521 or placebo administration, participants will be pre-treated with an oral antihistamine, selected by the investigator from the list of approved antihistamines that is available in that country. Approved antihistamines are: diphenhydramine 50 mg by mouth (PO), chlorpheniramine 8 mg PO, or hydroxyzine 50 mg PO.

CHB Participants: Cohort 3bCHB Participants: Cohort 3cCHB Participants: Cohort 4bCHB Participants: Cohort 4cNHV Participants: Cohort 1NHV Participants: Cohort 2NHV Participants: Cohort 3NHV Participants: Cohort 4NHV Participants: Cohort 5NHV Participants: Cohort 6NHV Participants: Placebo

Participants take entecavir OR tenofovir daily throughout the study.

CHB Participants: Cohort 3cCHB Participants: Cohort 4c

Approximately two hours prior to ARC-521 or placebo administration, participants will be pre-treated with acetaminophen (500 - 1000 mg PO, per local strength availability).

CHB Participants: Cohort 3bCHB Participants: Cohort 3cCHB Participants: Cohort 4bCHB Participants: Cohort 4cNHV Participants: Cohort 1NHV Participants: Cohort 2NHV Participants: Cohort 3NHV Participants: Cohort 4NHV Participants: Cohort 5NHV Participants: Cohort 6NHV Participants: Placebo

Participants take entecavir OR tenofovir daily throughout the study.

CHB Participants: Cohort 3cCHB Participants: Cohort 4c

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Male or female, 18 to 55 years of age inclusive (NHVs) or 18-65 years of age inclusive (CHBs), at the time of informed consent
  • Able to provide written informed consent prior to the performance of any study specific procedures
  • Body mass index (BMI) between 19.0 and 35.0 kg/m2, inclusive
  • A 12-lead ECG at Screening and pre-dose assessment that, in the opinion of the investigator, has no abnormalities that compromise participant's safety in this study
  • Must use 2 effective methods of contraception (double barrier contraception or hormonal contraceptive along with a barrier contraceptive (both male and female partners)
  • Have suitable venous access for blood sampling
  • No abnormal finding of clinical relevance at the Screening evaluation (NHVs only)
  • Have a diagnosis of HbeAg-negative chronic HBV infection (CHB patients only)
  • Treatment-naive or currently on entecavir/tenofovir for 6 months or longer

You may not qualify if:

  • Pregnant or lactating
  • Acute signs of hepatitis/other infection at Screening or at baseline
  • Use within last 14 days or anticipated requirement for anticoagulants, systemic corticosteroids, immunomodulators, or immunosuppressants
  • Use of prescription medication within 14 days prior to study treatment that in the opinion of the PI or the Sponsor would interfere with study conduct.
  • Known diagnosis of non-alcoholic steatohepatitis \[NHVs only\] or familial hypercholesterolemia
  • Taking interferon alpha (INFalpha) within 6 months of screening \[CHBs only\]
  • History of poorly controlled autoimmune disease or history of autoimmune hepatitis
  • Human immunodeficiency virus (HIV) infection
  • Seropositive for HBV (NHVs only) or hepatitis C virus (HCV), and/or history of delta virus hepatitis
  • Hypertension defined as blood pressure \> 170/100 mmHg at screening \[NHVs only\]
  • A history of cardiac rhythm disturbances
  • Family history of congenital long QT syndrome, Brugada syndrome or unexplained sudden cardiac death
  • Symptomatic heart failure, unstable angina, myocardial infarction, severe cardiovascular disease within 6 months prior to study entry
  • History of malignancy within the last 5 years except for adequately treated basal cell carcinoma, squamous cell skin cancer, superficial bladder tumors, or in situ cervical cancer
  • History of major surgery within 3 months of Screening
  • +12 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Research Site 1

Grafton, Auckland, 1011, New Zealand

Location

MeSH Terms

Conditions

Hepatitis B

Interventions

Histamine AntagonistsAcetaminophenentecavirTenofovir

Condition Hierarchy (Ancestors)

Blood-Borne InfectionsCommunicable DiseasesInfectionsHepadnaviridae InfectionsDNA Virus InfectionsVirus DiseasesHepatitis, Viral, HumanHepatitisLiver DiseasesDigestive System Diseases

Intervention Hierarchy (Ancestors)

Histamine AgentsNeurotransmitter AgentsMolecular Mechanisms of Pharmacological ActionPharmacologic ActionsChemical Actions and UsesPhysiological Effects of DrugsAcetanilidesAnilidesAmidesOrganic ChemicalsAniline CompoundsAminesOrganophosphonatesOrganophosphorus CompoundsAdeninePurinesHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-RingHeterocyclic Compounds

Limitations and Caveats

The ARC-521 Injection development program was terminated early for regulatory and business reasons secondary to findings occurring in a non-clinical toxicology study. Program termination was not due to safety findings in humans.

Results Point of Contact

Title
Chief Operating Officer
Organization
Arrowhead Pharmaceuticals, Inc.

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 8, 2016

First Posted

June 13, 2016

Study Start

June 1, 2016

Primary Completion

November 1, 2016

Study Completion

November 1, 2016

Last Updated

January 12, 2026

Results First Posted

September 25, 2018

Record last verified: 2025-12

Data Sharing

IPD Sharing
Will not share

Locations