Influence of Sedentary Time on Skeletal Muscle Protein Metabolism

NCT02624011 | Completed

• 1 other identifier: ERN_15-1238
• Study Type: interventional
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

This study will investigate the influence of increased sedentary time on long-term measures of muscle protein synthesis and metabolic health. The investigators will test the hypothesis that increased time spent in sedentary behaviours will lead to a reduction in long-term measures of muscle protein synthesis and compromised metabolic health.

Conditions

Sedentary Lifestyle

Outcome Measures

Primary Outcomes (1)

• Muscle protein synthesis (FSR %/day)

  Long-term muscle protein synthesis (FSR %/day) will be determined using deuterium oxide (D2O) to compare muscle protein synthesis rates over the first 7 day period and the second 7 day period.

  0-14 days

Secondary Outcomes (7)

• Rate of fat oxidation at rest (g/min)

  For 20 minutes at day 7 and day 14

• Rate of fat oxidation during an oral glucose tolerance test (g/min)

  At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14

• Rate of carbohydrate oxidation at rest (g/min)

  For 20 minutes at day 7 and day 14

• Rate of carbohydrate oxidation during an oral glucose tolerance test (g/min)

  At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14

• Blood glucose concentration

  At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14

Study Arms (1)

Physical inactivity

EXPERIMENTAL

Study arm consisting of 7 days of habitual physical activity followed by 7 days of step reduction and exercise cessation.

Behavioral: 7 days of habitual physical activity followed by 7 days of reduced physical activity

Interventions

7 days of habitual physical activity followed by 7 days of reduced physical activity BEHAVIORAL

Young, active participants will undergo a 7 day period of habitual physical activity followed by a 7 day period of step reduction and exercise cessation.

Physical inactivity

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Body mass index (18.5-29.99 kg/m2)
• Active - defined as: ≥ 7000 steps per day (assessed during screening) and taking part in regular exercise (defined as participation in ≥ 3 sessions of aerobic and/or resistance exercise type sessions a week for ≥ 6 months prior to recruitment)
• Good general health

You may not qualify if:

• Lidocaine allergy
• Hypertension (≥140/90 mmHg)
• Current participation in another clinical study
• Previous participation in this study
• Bleeding disorder/s
• Current or recent smoker
• Past history of substance abuse and/or taking prescription or non-prescription medication (e.g., beta-blockers, insulin or thyroxine) or supplements that may influence normal metabolic responses.
• Participants who have previously (within 5 years of the present study) had 4 or more muscle biopsies obtained from the thigh quadriceps region will be ineligible.

Sponsors & Collaborators

• University of Birmingham: lead
• Maastricht University: collaborator

Study Sites (1)

School of Sport Exercise and Rehabilitation Sciences, University of Birmingham

Birmingham, B15 2TT, United Kingdom

Location

MeSH Terms

Conditions

Sedentary Behavior

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Brandon J Shad, BSc

  University of Birmingham

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 2, 2015
• First Posted: December 8, 2015
• Study Start: March 1, 2016
• Primary Completion: April 1, 2017
• Study Completion: February 1, 2018
• Last Updated: October 11, 2018

Record last verified: 2018-10

Locations

GB (1)