A Study on the Mechanism of Cough Hypersensitivity

NCT02591550 | Unknown DMC

• 1 other identifier: coughlongli
• Study Type: observational
• Target: 300
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is described as follows,

1. 1.To establish a validated method to test cough reflex sensitivity conducted by transient receptor potential vanilloid 1(TRPV1).
2. 2.To observe the variance of cough reflex sensitivity conducted by transient receptor potential ankyrin 1(TRPA1) of chronic patients and the relationship between cough reflex sensitivity conducted by TRPA1 and conducted by TRPV1.
3. 3.To study the distribution of TRPA1 and TRPV1 channels and their relationship to cough reflex sensitivity.

Conditions

Cough

Keywords

chronic cough; cough reflex sensitivity; transient receptor potential vanilloid 1; transient receptor potential ankyrin 1

Outcome Measures

Primary Outcomes (1)

• TRPA1,TRPV1 channels expression level of chronic cough patients and its relationship with cough reflex sensitivity

  Complete AITC cough provocation test the day subjects enter the study,then complete CAP cough provocation test after 3 days,record the threshold of cap and aitc concentration(mmol/L) when it cause subjects cough equal to or more than 5 times.Measure the expression level of TRPA1 and TRPV1 of biopsy sample from chronic cough patients and healthy controls by immunofluoresent assay 7 days post bronchoscopy.Analyze the statistic by spss18.0.

  7 days post-bronchoscopy

Secondary Outcomes (1)

• The relationship between CAP cough reflex sensitivity test conducted by TRPV1 and AITC cough reflex sensitivity test conducted by TRPA1

  7 days post-bronchoscopy

Study Arms (3)

patients with normal cough sensitivity

patients with high cough sensitivity

healty controls

Eligibility Criteria

• Age: 17 Years - 70 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Chronic cough group:nonsmokers with chronic cough lasting ≥ 8 weeks characterized by irritating dry cough or small amount of sputum, sensitive to fumes，dust, cold air ,and normal chest x-ray were enrolled. Healthy control:healthy volunteers presented our hospital for healthy examination were enrolled.

You may qualify if:

• chronic cough group:
• cough lasting ≥ 8 weeks,characterized by irritating dry cough.
• sensitive to fumes，dust,the odorous and cold air.
• with normal chest x-rays. 4.17-70 years old.
• without smoking history. healty controls group: 1.17-70 years old. 2.without smoking hitory or stop smoking for more than 2 years. 3.without chronic cough. 4.without chronic respiratory diseases. 5.without chronic heart, liver, kidney,and autoimmune disease. 6.with normal chest x-rays. 7.with normal pulmonary ventilation function,and histamine challenge test showed negative result.

You may not qualify if:

• chronic cough group and healty controls group:
• with respiratory tract infection within 8 weeks.
• with chronic respiratory diseases or severe heart, liver, kidney,and autoimmune disease.
• using Angiotensin-Converting Enzyme Inhibitors(ACEI),bronchodilators,glucocorticosteroid,antihistaminics within one week.
• women during pregnancy or lactation.
• patients with malignant tumours.

Sponsors & Collaborators

• Kefang Lai: lead

Study Sites (1)

Guangzhou Institute of Respiratory Disease

Guangzhou, Guangdong, 520120, China

RECRUITING

Related Publications (2)

• Lai K, Long L, Yi F, Tang J, Chen Z, Chen F, Zhou J, Peng W, Zhang L, Li H, Zhan W, Chen R, Luo W, Chen Q, Chung KF, Zhong N. Age and Sex Distribution of Chinese Chronic Cough Patients and Their Relationship With Capsaicin Cough Sensitivity. Allergy Asthma Immunol Res. 2019 Nov;11(6):871-884. doi: 10.4168/aair.2019.11.6.871.

  PMID: 31552721 DERIVED

• Long L, Yao H, Tian J, Luo W, Yu X, Yi F, Chen Q, Xie J, Zhong N, Chung KF, Lai K. Heterogeneity of cough hypersensitivity mediated by TRPV1 and TRPA1 in patients with chronic refractory cough. Respir Res. 2019 Jun 6;20(1):112. doi: 10.1186/s12931-019-1077-z.

  PMID: 31170994 DERIVED

Biospecimen

Retention: SAMPLES WITHOUT DNA

mucosa of upper trachea

MeSH Terms

Conditions

Cough; Chronic Cough

Condition Hierarchy (Ancestors)

Respiration Disorders; Respiratory Tract Diseases; Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Central Study Contacts

Kefang Lai, PhD

CONTACT

8620-83062893; klai@163.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: October 21, 2015
• First Posted: October 29, 2015
• Study Start: March 1, 2015
• Primary Completion: December 1, 2017
• Study Completion: December 1, 2017
• Last Updated: October 29, 2015

Record last verified: 2015-10

Locations

CN (1)