NCT02573662

Brief Summary

Introduction This study evaluates the effect on glucose, lipid and bone metabolism following knee orthopedic procedures in healthy and physically active individuals. The sedentary rehabilitation period following these procedures may impact negatively on glucose, lipid and bone metabolic pathways, whereas the more physically active rehabilitation period instituted 6 weeks after surgery is hypothesized to impact positively. Perspective This study will establish whether the well-known effects on glucose, lipid and bone metabolism of a sedentary lifestyle can be observed already following 6 weeks of physical inactivity in otherwise healthy and physically fit young and middle aged individuals. Investigators will thereby add knowledge to previous findings following strict bed-rest in healthy individuals on glucose, lipid and bone metabolism. In a clinical perspective it is important to examine the extent to which healthy individuals deteriorate in various metabolic pathways to better understand the pathophysiology behind these defects both in healthy individuals and in patients, who undergo bed rest or an equal reduction in physical activity as part of their rehabilitation. Study design 16 physical active non-diabetic individuals of age 18 - 50 years who are undergoing knee surgical procedures at the Arthroscopic Center at Amager/Hvidovre Hospitals are recruited as cases for this case-control study. 10 non-diabetic control subjects matched for age, gender and physical activity are recruited to establish a reference level.

  • The individuals will bring in morning spot urine for measurement of soluble urokinase plasminogen activating receptor (suPAR), creatinine, albumin and orosomucoid. Weight and height and waist and hip circumference will be measured.
  • Oral glucose tolerance test (OGTT) with ingestion of 75 g glucose during 5 min from baseline (0 min). Plasma for glucose, insulin, C-peptide, non-esterified fatty acid (NEFA) will be drawn
  • Before OGTT blood will be drawn for measurement of HbA1c, total cholesterol, LDL, HDL, triglyceride, Na, K, creatinine, hemoglobin (HgB), C-reactive protein (CRP), leukocytes, alanintransaminase (ALAT), alkaline phosphatase, Ca++, D vitamin, TSH, bone turnover markers (BTM), suPAR, interleukin 6 (IL6), TNFa, high-sensitivity C-reactive protein (hsCRP), lipid density profiling and lipid particle size.
  • Dual energy X-ray absorptiometry (DXA) of hip, lumbar columna, visceral and subcutaneous fat is measured by Hologic Discovery scanner.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
16

participants targeted

Target at below P25 for all trials

Timeline
Completed

Started Apr 2015

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 13, 2015

Completed
19 days until next milestone

Study Start

First participant enrolled

April 1, 2015

Completed
6 months until next milestone

First Posted

Study publicly available on registry

October 12, 2015

Completed
4 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2016

Completed
4 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2016

Completed
Last Updated

November 10, 2015

Status Verified

November 1, 2015

Enrollment Period

10 months

First QC Date

March 13, 2015

Last Update Submit

November 8, 2015

Conditions

Keywords

Metabolic complicationsType 2 diabetesSedentary lifestyleLow grade inflammationLipid metabolismGlucose metabolismBone metabolismPhysical inactivity

Outcome Measures

Primary Outcomes (1)

  • Change in insulin sensitivity

    Glucose metabolic: -insulin sensitivity as measured by MinModel-OGTT

    Baseline to week 6

Secondary Outcomes (5)

  • Change in glucose metabolism

    Baseline to week 6 and to week 16

  • Lipid metabolism

    Baseline to week 6 and to week 16

  • Bone Metabolism

    Baseline to week 6 and to week 16

  • Atherosclerotic markers

    Baseline to week 6 and to week 16

  • Change in insulin sensitivity

    week 6 and to week 16

Study Arms (2)

Case subjects

Physical active non-diabetic individuals of age 18 to 50 years, who are undergoing knee surgical procedures at the Arthroscopic Center at Amager/Hvidovre Hospitals are recruited as cases for this case-control study. OGTT, blood- and urine sampling and DXA scans will be performed 3 times throughout the study period.

Biological: Blood and Urine samplingRadiation: DXA scanBiological: OGTT

Control group

Non-diabetic individuals matched for age, gender and physical activity are recruited as control subjects to establish a reference level likely to image the cases before they experienced their knee injury. Blood- and urine sampling, OGTT and DXA scans will be carried out 1-3 times for each control subject. No lifestyle intervention is implemented.

Biological: Blood and Urine samplingRadiation: DXA scanBiological: OGTT

Interventions

Blood and Urine Sampling

Case subjectsControl group
DXA scanRADIATION

Scan of Lumbar columna and neck of femur/hip Scan of visceral/subcutaneous fatty tissue

Case subjectsControl group
OGTTBIOLOGICAL

Oral Glucose Tolerance Test for Minimal Model analysis for insulin sensitivity and Lipid oxidation

Case subjectsControl group

Eligibility Criteria

Age18 Years - 50 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)
Sampling MethodNon-Probability Sample
Study Population

Sixteen physical active non-diabetic individuals of age 18 to 50 years who are undergoing knee surgical procedures at the Arthroscopic Center at Amager/Hvidovre Hospitals are recruited as cases for this case-control study. Ten non-diabetic individuals matched for age, gender and physical activity are recruited as control subjects. The pre-surgical physical activity level is expected to be lower than their habitual physical activity, however more than that which would be expected from individuals with a sedentary life-style. At least 6 of the control subjects will be examined another two times, that is, after 6 weeks and after 16 weeks for the purpose of time controls.

You may qualify if:

  • Individuals of age 18 to 50 years
  • Physically active \>/= 1.5 hours/week up till baseline examination
  • Physically active at least 2.5 hours/week before knee injury.
  • The participants should undergo one of the following orthopaedic surgical procedures; Medial patella-femoral ligament (MPFL) surgery, Anterior Crusiate Ligament (ACL) and/or Posterior Crusiate Ligament (PCL) surgery, Knee Cartilage Injury surgery
  • Informed written consent

You may not qualify if:

  • BMI \>/= 30 kg/m2
  • Not physically active before surgical procedure as measured by questionnaire
  • Known diabetes mellitus or HbA1c \>/= 48 mol/mol
  • Clinical significant anemia, liver or kidney disease as judged by the sponsor or principal investigator.
  • Thyroid function abnormality (TSH \< 0.1 or TSH \> 10)
  • Calcium metabolic derangement, Ca++ \< 1.1 or Ca++ \> 1.40
  • Known osteoporosis

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Amager University Hospital

Copenhagen, 2300, Denmark

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Blood and Urine sampling

MeSH Terms

Conditions

Glucose Metabolism DisordersLipid Metabolism DisordersSedentary BehaviorDiabetes Mellitus, Type 2

Interventions

Blood Specimen CollectionAbsorptiometry, PhotonGlucose Tolerance Test

Condition Hierarchy (Ancestors)

Metabolic DiseasesNutritional and Metabolic DiseasesBehaviorDiabetes MellitusEndocrine System Diseases

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative TechniquesRadiographyDiagnostic ImagingDensitometryPhotometryChemistry Techniques, AnalyticalBlood Chemical AnalysisClinical Chemistry TestsDiagnostic Techniques, Endocrine

Study Officials

  • Per Hölmich, MD, DMSc

    Amager Hospital

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Steen B Haugaard, MD, DMSc

CONTACT

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
INDIV
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 13, 2015

First Posted

October 12, 2015

Study Start

April 1, 2015

Primary Completion

February 1, 2016

Study Completion

June 1, 2016

Last Updated

November 10, 2015

Record last verified: 2015-11

Locations