NCT02549144

Brief Summary

In this study the investigators aimed at measuring the effect of a two-week high-fat/high-cholesterol (HFHC) normocaloric diet on HDL particles biochemical composition and HDL function compared to a two-week low-fat/low-cholesterol (LFLC) normocaloric diet.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
14

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jan 2015

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2015

Completed
8 months until next milestone

First Submitted

Initial submission to the registry

September 3, 2015

Completed
12 days until next milestone

First Posted

Study publicly available on registry

September 15, 2015

Completed
2 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2015

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2015

Completed
Last Updated

January 6, 2022

Status Verified

December 1, 2021

Enrollment Period

10 months

First QC Date

September 3, 2015

Last Update Submit

December 16, 2021

Conditions

Keywords

HDL biochemical composition and function

Outcome Measures

Primary Outcomes (1)

  • Dichlorofluorescein fluorescence slope

    14 days

Secondary Outcomes (2)

  • hydroxyeicosatetraenoic acid

    14 days

  • hydroxyoctadecadienoic acid

    14 days

Study Arms (2)

High fat/cholesterol diet

EXPERIMENTAL

High-fat/high-cholesterol (HFHC) normocaloric diet for 14 days.

Dietary Supplement: High fat/cholesterol diet

Low fat/cholesterol diet

EXPERIMENTAL

Low-fat/low-cholesterol (LFLC) normocaloric diet for 14 days.

Dietary Supplement: Low fat/cholesterol diet

Interventions

Low fat/cholesterol dietDIETARY_SUPPLEMENT

The low fat/cholesterol diet had 10% calories from fat and 150-200 mg of cholesterol per day.

Low fat/cholesterol diet
High fat/cholesterol dietDIETARY_SUPPLEMENT

The high fat/cholesterol diet had 40% calories from fat and 250-300 mg of cholesterol per day

High fat/cholesterol diet

Eligibility Criteria

Age18 Years - 40 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Subjects ≥ 18 and ≤40 years of age
  • Lean or overweight (BMI: 18 to 29,9 kg/m2)
  • Normal liver and kidney function
  • Normal thyroid function
  • Stable weight in the last 3 months
  • Read and understood the informed consent form and signed it voluntarily

You may not qualify if:

  • Liver, heart, kidney, lung, infectious, neurological, psychiatric, endocrine, immunological or neoplastic diseases
  • Chronic use of drugs or vitamin supplements
  • Smoking
  • Pregnancy or lactation

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Azienda Ospedaliero-Universitaria Pisana

Pisa, PI, 56127, Italy

Location

Related Publications (2)

  • Morgantini C, Trifiro S, Trico D, Meriwether D, Baldi S, Mengozzi A, Reddy ST, Natali A. A short-term increase in dietary cholesterol and fat intake affects high-density lipoprotein composition in healthy subjects. Nutr Metab Cardiovasc Dis. 2018 Jun;28(6):575-581. doi: 10.1016/j.numecd.2018.03.005. Epub 2018 Mar 19.

  • Trico D, Trifiro S, Mengozzi A, Morgantini C, Baldi S, Mari A, Natali A. Reducing Cholesterol and Fat Intake Improves Glucose Tolerance by Enhancing beta Cell Function in Nondiabetic Subjects. J Clin Endocrinol Metab. 2018 Feb 1;103(2):622-631. doi: 10.1210/jc.2017-02089.

Study Officials

  • Andrea Natali, Prof

    Azienda Ospedaliero, Universitaria Pisana

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Professor Andrea Natali

Study Record Dates

First Submitted

September 3, 2015

First Posted

September 15, 2015

Study Start

January 1, 2015

Primary Completion

November 1, 2015

Study Completion

December 1, 2015

Last Updated

January 6, 2022

Record last verified: 2021-12

Locations