NCT02509494

Brief Summary

The purpose of this study is the evaluation of the safety and immunogenicity of two candidate Ebola vaccines Ad26.ZEBOV and MVA-BN-Filo, in a 2-dose heterologous regimen.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,023

participants targeted

Target at P75+ for phase_3

Timeline
Completed

Started Sep 2015

Typical duration for phase_3

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 24, 2015

Completed
1 month until next milestone

First Posted

Study publicly available on registry

July 28, 2015

Completed
2 months until next milestone

Study Start

First participant enrolled

September 30, 2015

Completed
3.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 28, 2019

Completed
5 days until next milestone

Study Completion

Last participant's last visit for all outcomes

July 3, 2019

Completed
3 years until next milestone

Results Posted

Study results publicly available

July 18, 2022

Completed
Last Updated

July 18, 2022

Status Verified

June 1, 2022

Enrollment Period

3.7 years

First QC Date

June 24, 2015

Results QC Date

June 22, 2022

Last Update Submit

June 22, 2022

Conditions

Keywords

VaccineEbolaEbola virus diseaseEVDHemorrhagic feverSierra LeoneHuman adenovirus serotype 26 (Ad26) encoding the Ebola virus Mayinga variant glycoprotein (Ad26.ZEBOV)SafetyImmunogenicityModified Vaccinia Virus Ankara - Bavarian Nordic Filo-vector(MVA-BN Filo)

Outcome Measures

Primary Outcomes (19)

  • Stages 1 and 2: Number of Participants With Solicited Local Adverse Events (AEs) (Day 8)

    Number of participants with solicited local AEs were reported. Solicited local AEs were pre-defined local (at the injection site) AEs for which participants were specifically questioned and which were noted by participants in their diary for 7 days post vaccination. Solicited local AEs were: injection site pain/tenderness, erythema, induration/swelling, itching at the vaccination site.

    7 days post dose 1 (Day 8)

  • Stages 1 and 2: Number of Participants With Solicited Local AEs (Day 64)

    Number of participants with solicited local AEs were reported. Solicited local AEs were pre-defined local (at the injection site) AEs for which participants were specifically questioned and which were noted by participants in their diary for 7 days post vaccination. Solicited local AEs were: injection site pain/tenderness, erythema, induration/swelling, itching at the vaccination site.

    7 days post dose 2 (Day 64)

  • Stage 1: Number of Participants With Solicited Local AEs (Day 738)

    Number of participants with solicited local AEs were reported. Solicited local AEs were pre-defined local (at the injection site) AEs for which participants were specifically questioned and which were noted by participants in their diary for 7 days post vaccination. Solicited local AEs were: injection site pain/tenderness, erythema, induration/swelling, itching at the vaccination site.

    7 days post dose 3 (Day 738)

  • Stages 1 and 2: Number of Participants With Solicited Systemic AEs (Day 8)

    Number of participants with solicited systemic AEs were reported. Solicited systemic AEs included body temperature, vomiting, reduced activity, somnolence, fatigue, irritability/fussiness/crying/screaming, and loss of appetite (for preverbal children/infants) and body temperature, nausea/vomiting, fatigue/malaise, muscle pain, chills, joint pain and headache (for young children, adolescents, and adults).

    7 days post dose 1 (Day 8)

  • Stages 1 and 2: Number of Participants With Solicited Systemic AEs (Day 64)

    Number of participants with solicited systemic AEs were reported. Solicited systemic AEs included body temperature, vomiting, reduced activity, somnolence, fatigue, irritability/fussiness/crying/screaming, and loss of appetite (for preverbal children/infants) and body temperature, nausea/vomiting, fatigue/malaise, muscle pain, chills, joint pain and headache (for young children, adolescents, and adults).

    7 days post dose 2 (Day 64)

  • Stage 1: Number of Participants With Solicited Systemic AEs (Day 738)

    Number of participants with solicited systemic AEs were reported. Solicited systemic AEs included body temperature, vomiting, reduced activity, somnolence, fatigue, irritability/fussiness/crying/screaming, and loss of appetite (for preverbal children/infants) and body temperature, nausea/vomiting, fatigue/malaise, muscle pain, chills, joint pain and headache (for young children, adolescents, and adults).

    7 days post dose 3 (Up to Day 738)

  • Stages 1: Number of Participants With Serious Adverse Events (SAEs)

    Number of Participants with SAEs were reported. SAE is any untoward medical occurrence that at any dose may result in death, is life-threatening, requires inpatient hospitalization or prolongation of existing hospitalization, results in persistent or significant disability/incapacity, is a congenital anomaly/birth defect, is a suspected transmission of any infectious agent via a medicinal product.

    Up to 36 months

  • Stages 2: Number of Participants With SAEs

    Number of Participants with SAEs were reported. SAE is any untoward medical occurrence that at any dose may result in death, is life-threatening, requires inpatient hospitalization or prolongation of existing hospitalization, results in persistent or significant disability/incapacity, is a congenital anomaly/birth defect, is a suspected transmission of any infectious agent via a medicinal product.

    Up to 24 months

  • Stage 1: Number of Participants With Unsolicited AEs (Day 759)

    Number of participants with unsolicited AEs were reported. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    28 days post booster dose (Day 759)

  • Stage 1: Number of Participants With Unsolicited AEs (Day 29)

    Number of participants with unsolicited AEs were reported. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    28 days post dose 1 (Day 29)

  • Stage 2: Number of Participants With Unsolicited AEs (Day 29)

    Number of participants with unsolicited AEs were reported. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    28 days post dose 1 (Day 29)

  • Stage 1: Number of Participants With Unsolicited AEs (Day 85)

    Number of participants with unsolicited AEs were reported. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    28 days post dose 2 (Day 85)

  • Stage 2: Number of Participants With Unsolicited AEs (Day 85)

    Number of participants with unsolicited AEs were reported. Unsolicited AEs were all AEs for which the participant is not specifically questioned in the participant diary.

    28 days post dose 2 (Day 85)

  • Stage 1: Number of Participants With Deaths

    Number of participants with deaths were reported.

    Up to 36 months

  • Stage 2: Number of Participants With Deaths (Children and Adolescents)

    Number of participants (children and adolescents) with deaths were reported.

    Up to 12 months

  • Stage 2: Number of Participants With Deaths (Adults)

    Number of participants (adults) with deaths were reported.

    Up to 24 months

  • Stage 1: Number of Participants With Immediate Reportable Event (IREs)

    Number of participants with IREs were reported. The following list of neuroinflammatory disorders are categorized as IREs: Cranial nerve disorders, including paralyses/paresis, optic neuritis, multiple sclerosis, transverse myelitis, guillain-Barré syndrome, acute disseminated encephalomyelitis, including site specific variants, myasthenia gravis and Lambert-Eaton myasthenic syndrome, immune-mediated peripheral neuropathies and plexopathies, narcolepsy, isolated paresthesia of greater than (\>) 7 days duration.

    Up to 36 months

  • Stage 2: Number of Participants With IREs (Children and Adolescents)

    Number of participants (children and adolescents) with IREs were reported. The following list of neuroinflammatory disorders are categorized as IREs: Cranial nerve disorders, including paralyses/paresis, optic neuritis, multiple sclerosis, transverse myelitis, guillain-Barré syndrome, acute disseminated encephalomyelitis, including site specific variants, myasthenia gravis and Lambert-Eaton myasthenic syndrome, immune-mediated peripheral neuropathies and plexopathies, narcolepsy, isolated paresthesia of greater than (\>) 7 days duration.

    Up to 12 months

  • Stage 2: Number of Participants With IREs (Adults)

    Number of participants (adults) with IREs were reported. The following list of neuroinflammatory disorders are categorized as IREs: Cranial nerve disorders, including paralyses/paresis, optic neuritis, multiple sclerosis, transverse myelitis, guillain-Barré syndrome, acute disseminated encephalomyelitis, including site specific variants, myasthenia gravis and Lambert-Eaton myasthenic syndrome, immune-mediated peripheral neuropathies and plexopathies, narcolepsy, isolated paresthesia of greater than (\>) 7 days duration.

    Up to 24 months

Secondary Outcomes (1)

  • Stages 1 and 2: Geometric Mean Concentrations (GMCs) of Binding Antibody Levels Against Ebola Virus Glycoprotein (EBOV GP) Measured Using Enzyme-linked Immunosorbent Assay (ELISA)

    21 days post-dose 2 (Day 78)

Study Arms (5)

Stage 1: Active vaccination

EXPERIMENTAL

Ad26.ZEBOV will be administered as a 0.5 milliliter (mL) intramuscular (IM) injection (Dose 1); MVA-BN-Filo will be administered as a 0.5 mL IM injection (Dose 2). The booster vaccination using Ad26.ZEBOV will be administered as a 0.5 mL IM injection (2 years post Dose 1).

Biological: Ad26.ZEBOVBiological: MVA-BN-Filo

Stage 2: Active vaccination

EXPERIMENTAL

Ad26.ZEBOV will be administered as a 0.5 mL IM injection (Dose 1); MVA-BN-Filo will be administered as a 0.5 mL IM injection (Dose 2).

Biological: Ad26.ZEBOVBiological: MVA-BN-Filo

Stage 2: Active vaccination for children

EXPERIMENTAL

Ad26.ZEBOV will be administered as a 0.5 mL IM injection (Dose 1); MVA-BN-Filo will be administered as a 0.5 mL IM injection (Dose 2). Children aged less than 2 years at randomization will receive a booster dose of vaccination at 3 months post Dose 2 with Placebo.

Biological: Ad26.ZEBOVBiological: MVA-BN-Filo

Stage 2: Control vaccination

ACTIVE COMPARATOR

MenACWY will be administered as a 0.5 mL IM injection on Day 1 (Dose 1) and placebo on Day 57 (Dose 2).

Biological: MenACWYBiological: Placebo

Stage 2: Control vaccination for children

ACTIVE COMPARATOR

MenACWY will be administered as a 0.5 mL IM injection on Day 1 (Dose 1) and placebo on Day 57 (Dose 2). Children aged less than 2 years at randomization will receive a booster dose of MenACWY vaccination at 3 months post Dose 2 with MenACWY.

Biological: MenACWYBiological: Placebo

Interventions

Ad26.ZEBOVBIOLOGICAL

Ebola Zaire vaccine, replication incompetent vaccine, sterile suspension of 0.5 milliliter (mL) intramuscular (IM) injection of 5\*10\^10 viral particles.

Stage 1: Active vaccinationStage 2: Active vaccinationStage 2: Active vaccination for children
MVA-BN-FiloBIOLOGICAL

MVA-BN-Filo- is a non-replicating vaccine, 0.5 mL IM injection of 1\*10\^8 Infectious Unit (Inf. U.).

Stage 1: Active vaccinationStage 2: Active vaccinationStage 2: Active vaccination for children
MenACWYBIOLOGICAL

MenACWY is a WHO-prequalified Meningococcal Group A, C, W135 and Y conjugate vaccine.

Stage 2: Control vaccinationStage 2: Control vaccination for children
PlaceboBIOLOGICAL

0.9% saline for injection.

Stage 2: Control vaccinationStage 2: Control vaccination for children

Eligibility Criteria

Age1 Year+
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Documented community engagement from community leader and a signed inform consent form (ICF) from each participant must be available
  • Participant Stage 1 must be 18 years or older at screening and be resident in selected study community with no intention to move from study area within the next 5 months
  • Participant must be healthy with no abnormalities in laboratory screening tests within 28 days before Dose 1 vaccination
  • Female participants of childbearing potential must use adequate birth control measures and must have a negative pregnancy test at screening and immediately prior to each study vaccination
  • Participant must pass the test of understanding (TOU)
  • One year or older at screening (children of enrolled parents are eligible)
  • Parent/legal guardian (for children) must pass the TOU before signing the ICF
  • Subjects aged 7 years and older will be asked to give positive assent in the presence of a witness

You may not qualify if:

  • Diagnosed with EVD or under quarantine/exposed to Ebola or body temperature equal of \>= 38 degree Celsius (fever)
  • Having an acute illness (mild in nature that can be treated at home) or any clinically significant acute/chronic medical condition or having a decreased number of red blood cells/hemoglobin in the blood (anemia)
  • Previously participated in another Ebola interventional study or received any Ad26/MVA-based candidate vaccine
  • Vaccinated with live attenuated vaccines within 30 days or with inactivated vaccines 15 days before Dose 1 vaccination
  • Treated with an immunosuppressive drug at the time of screening
  • \- Children up to 5 years of age with severe malnutrition (underweight or Z-score weight \<2)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Unknown Facility

Freetown, Sierra Leone

Location

Related Publications (5)

  • Barry H, Lhomme E, Surenaud M, Nouctara M, Robinson C, Bockstal V, Valea I, Somda S, Tinto H, Meda N, Greenwood B, Thiebaut R, Lacabaratz C. Helminth exposure and immune response to the two-dose heterologous Ad26.ZEBOV, MVA-BN-Filo Ebola vaccine regimen. PLoS Negl Trop Dis. 2024 Apr 11;18(4):e0011500. doi: 10.1371/journal.pntd.0011500. eCollection 2024 Apr.

  • Manno D, Patterson C, Drammeh A, Tetteh K, Kroma MT, Otieno GT, Lawal BJ, Soremekun S, Ayieko P, Gaddah A, Kamara AB, Baiden F, Afolabi MO, Tindanbil D, Owusu-Kyei K, Ishola D, Deen GF, Keshinro B, Njie Y, Samai M, Lowe B, Robinson C, Leigh B, Drakeley C, Greenwood B, Watson-Jones D. The Effect of Previous Exposure to Malaria Infection and Clinical Malaria Episodes on the Immune Response to the Two-Dose Ad26.ZEBOV, MVA-BN-Filo Ebola Vaccine Regimen. Vaccines (Basel). 2023 Aug 2;11(8):1317. doi: 10.3390/vaccines11081317.

  • Ishola D, Manno D, Afolabi MO, Keshinro B, Bockstal V, Rogers B, Owusu-Kyei K, Serry-Bangura A, Swaray I, Lowe B, Kowuor D, Baiden F, Mooney T, Smout E, Kohn B, Otieno GT, Jusu M, Foster J, Samai M, Deen GF, Larson H, Lees S, Goldstein N, Gallagher KE, Gaddah A, Heerwegh D, Callendret B, Luhn K, Robinson C, Leyssen M, Greenwood B, Douoguih M, Leigh B, Watson-Jones D; EBL3001 study group. Safety and long-term immunogenicity of the two-dose heterologous Ad26.ZEBOV and MVA-BN-Filo Ebola vaccine regimen in adults in Sierra Leone: a combined open-label, non-randomised stage 1, and a randomised, double-blind, controlled stage 2 trial. Lancet Infect Dis. 2022 Jan;22(1):97-109. doi: 10.1016/S1473-3099(21)00125-0. Epub 2021 Sep 13.

  • Afolabi MO, Ishola D, Manno D, Keshinro B, Bockstal V, Rogers B, Owusu-Kyei K, Serry-Bangura A, Swaray I, Lowe B, Kowuor D, Baiden F, Mooney T, Smout E, Kohn B, Otieno GT, Jusu M, Foster J, Samai M, Deen GF, Larson H, Lees S, Goldstein N, Gallagher KE, Gaddah A, Heerwegh D, Callendret B, Luhn K, Robinson C, Greenwood B, Leyssen M, Douoguih M, Leigh B, Watson-Jones D; EBL3001 study group. Safety and immunogenicity of the two-dose heterologous Ad26.ZEBOV and MVA-BN-Filo Ebola vaccine regimen in children in Sierra Leone: a randomised, double-blind, controlled trial. Lancet Infect Dis. 2022 Jan;22(1):110-122. doi: 10.1016/S1473-3099(21)00128-6. Epub 2021 Sep 13.

  • Mooney T, Smout E, Leigh B, Greenwood B, Enria L, Ishola D, Manno D, Samai M, Douoguih M, Watson-Jones D. EBOVAC-Salone: Lessons learned from implementing an Ebola vaccine trial in an Ebola-affected country. Clin Trials. 2018 Oct;15(5):436-443. doi: 10.1177/1740774518780678. Epub 2018 Jun 12.

MeSH Terms

Conditions

Hemorrhagic Fever, EbolaHemorrhagic Fevers, Viral

Interventions

MenACWY

Condition Hierarchy (Ancestors)

RNA Virus InfectionsVirus DiseasesInfectionsFiloviridae InfectionsMononegavirales Infections

Results Point of Contact

Title
Senior Director Clinical Franchise Lead
Organization
Janssen Vaccines & Prevention B.V.

Study Officials

  • Janssen Vaccines & Prevention B.V. Clinical Trial

    Janssen Vaccines & Prevention B.V.

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
OTHER
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 3
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 24, 2015

First Posted

July 28, 2015

Study Start

September 30, 2015

Primary Completion

June 28, 2019

Study Completion

July 3, 2019

Last Updated

July 18, 2022

Results First Posted

July 18, 2022

Record last verified: 2022-06

Locations