NCT02463513

Brief Summary

Approximately 10% of the world's population have a particular genetic makeup (known as the TT genotype) that may increase their risk of having higher blood pressure. Previous work conducted by the investigators research group at the University of Ulster, in collaboration with clinical colleagues from across Northern Ireland, in premature CVD patients and hypertensive adults generally has demonstrated that a dietary level of riboflavin (1.6mg/d) decreases blood pressure, specifically in those with the TT genotype. To date, the blood pressure lowering effects of higher doses of riboflavin in individuals with the TT genotype is not known. The aim of this study is to investigate whether supplementation with riboflavin at a low dose supplemental level (10mg/d) can decrease blood pressure more effectively than the dietary level (1.6mg/d) by optimising riboflavin status and normalising MTHFR activity. This aim will be achieved by conducting a double-blind placebo-controlled intervention study over a 16 week period. Participants will be recruited from cohorts screened for the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism. Those identified with the TT genotype (homozygous for the polymorphism) that wish to participate in this research will be asked to attend a baseline and week-16 appointment and will be asked to take a daily riboflavin (1.6 or 10mg/d) or placebo capsule for the duration of the study. At each appointment a blood sample will be taken and blood pressure, height, weight and waist circumference will be measured. If the results of this study show that intervention with a higher dose of riboflavin can lower blood pressure more effectively in individuals with the TT genotype this will have important implications for those responsible for the management of blood pressure. The findings will be of particular relevance in populations with a higher prevalence of the polymorphism.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
243

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Feb 2013

Longer than P75 for not_applicable

Geographic Reach
1 country

3 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

February 1, 2013

Completed
2.3 years until next milestone

First Submitted

Initial submission to the registry

June 2, 2015

Completed
2 days until next milestone

First Posted

Study publicly available on registry

June 4, 2015

Completed
2.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2017

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2017

Completed
Last Updated

May 13, 2016

Status Verified

May 1, 2016

Enrollment Period

4.8 years

First QC Date

June 2, 2015

Last Update Submit

May 12, 2016

Conditions

Keywords

RiboflavinBlood pressureMTHFRMethylenetetrahydrofolate reductase

Outcome Measures

Primary Outcomes (1)

  • Blood Pressure

    The aim of this study is to investigate whether a low dose supplemental level (10mg/d) of riboflavin can decrease blood pressure more effectively than the dietary level (1.6mg/d) by optimising riboflavin status and normalising MTHFR activity.

    16 weeks

Secondary Outcomes (5)

  • Erythrocyte Glutathione Reductase Activation Coefficient (EGRAC)

    16 weeks

  • Plasma Homocysteine

    16 weeks

  • Red cell folate

    16 weeks

  • Vitamin B12

    16 weeks

  • Vitamin B6

    16 weeks

Study Arms (3)

Treatment 1

PLACEBO COMPARATOR
Dietary Supplement: Placebo comparator

Treatment 2

ACTIVE COMPARATOR
Dietary Supplement: 1.6mg riboflavin (Vitamin B2)

Treatment 3

ACTIVE COMPARATOR
Dietary Supplement: 10mg riboflavin (Vitamin B2)

Interventions

Placebo comparatorDIETARY_SUPPLEMENT

2 week placebo washout + 16 weeks intervention (placebo)

Treatment 1

2 week placebo washout + 16 weeks intervention (1.6mg riboflavin per day)

Treatment 2

2 week placebo washout + 16 weeks intervention (10mg riboflavin per day)

Treatment 3

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Over 18 years old

You may not qualify if:

  • History of gastrointestinal; Hepatic; Renal or haematological disorders
  • Taking B-vitamin supplements (including multi-vitamins containing B-vitamins), anticonvulsant therapy or any other drugs known to interfere with folate/B-vitamin metabolism.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

Northern Ireland Clinical Research Facility (NICRF)

Belfast, N.Ireland, BT9 7AB, United Kingdom

Location

Human Intervention Studies Unit, University of Ulster

Coleraine, N.Ireland, BT52 1SA, United Kingdom

Location

Clinical Translational Research and Innovation Centre (C-TRIC)

Londonderry, N.Ireland, BT47 6SB, United Kingdom

Location

MeSH Terms

Interventions

Riboflavin

Intervention Hierarchy (Ancestors)

FlavinsPteridinesHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-RingHeterocyclic CompoundsHeterocyclic Compounds, 3-RingCoenzymesEnzymes and CoenzymesPigments, BiologicalBiological Factors

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 2, 2015

First Posted

June 4, 2015

Study Start

February 1, 2013

Primary Completion

December 1, 2017

Study Completion

December 1, 2017

Last Updated

May 13, 2016

Record last verified: 2016-05

Locations